FastQCFastQC Report
Thu 26 May 2016
SRR1544878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544878_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences806631
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT22620.28042562212461464TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT20790.2577386686105543Illumina Paired End PCR Primer 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT16760.2077777819101919TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.1397169213680109No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT10680.132402548377139No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9280.11504640907676496No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG8530.10574847730870744No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT253.416243E-546.036
CGAACGC206.3103304E-446.024
GCGAATA206.3103304E-446.039
ATTACGG206.3103304E-446.01
TAAACGA206.3103304E-446.020
GAATACG206.3103304E-446.012
ACGGGTC405.6079443E-946.04
ATACTCG206.3103304E-446.043
AATGCGG405.6079443E-946.01
ACAATCG253.416243E-546.044
CGGGAAC800.046.05
CGGTGAT253.416243E-546.05
GCGATGT551.8189894E-1246.08
GCGATGA850.046.08
GTATCGG453.092282E-1046.01
ACGTCCG206.3103304E-446.022
TTCGGGT253.416243E-546.011
CGATGCG600.046.09
CACAACG206.3103304E-446.011
GGTTCGC206.3103304E-446.07
Produced by FastQC (version 0.11.5)