##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544878_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	806631
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.909760472880414	34.0	33.0	34.0	31.0	34.0
2	33.243386381133384	34.0	33.0	34.0	31.0	34.0
3	33.29978143661724	34.0	34.0	34.0	31.0	34.0
4	36.582101357374064	37.0	37.0	37.0	35.0	37.0
5	36.575136586617674	37.0	37.0	37.0	35.0	37.0
6	36.68503937984035	37.0	37.0	37.0	35.0	37.0
7	36.664740135204326	37.0	37.0	37.0	35.0	37.0
8	36.58979012708413	37.0	37.0	37.0	35.0	37.0
9	38.35816500977523	39.0	39.0	39.0	37.0	39.0
10	38.08518393168624	39.0	39.0	39.0	35.0	39.0
11	37.12451542278936	39.0	35.0	39.0	35.0	39.0
12	37.01226707131266	39.0	35.0	39.0	35.0	39.0
13	36.99644695034036	39.0	35.0	39.0	35.0	39.0
14	38.17453953542574	40.0	35.0	41.0	35.0	41.0
15	38.241070576261016	40.0	35.0	41.0	35.0	41.0
16	38.24834527807634	40.0	35.0	41.0	35.0	41.0
17	38.33498464601534	40.0	36.0	41.0	35.0	41.0
18	38.314164717200306	40.0	36.0	41.0	35.0	41.0
19	38.204540861930674	40.0	35.0	41.0	35.0	41.0
20	38.109664766169416	40.0	35.0	41.0	35.0	41.0
21	38.05069480344792	40.0	35.0	41.0	35.0	41.0
22	38.03543751727866	40.0	35.0	41.0	35.0	41.0
23	37.991801703629044	40.0	35.0	41.0	35.0	41.0
24	37.947330315844546	40.0	35.0	41.0	35.0	41.0
25	37.90704547680414	40.0	35.0	41.0	35.0	41.0
26	37.84727713167483	40.0	35.0	41.0	35.0	41.0
27	37.84281908332311	40.0	35.0	41.0	35.0	41.0
28	37.88332087410476	40.0	35.0	41.0	35.0	41.0
29	37.87910333225477	40.0	35.0	41.0	35.0	41.0
30	37.776109522197885	39.0	35.0	41.0	35.0	41.0
31	37.69460137287062	39.0	35.0	41.0	35.0	41.0
32	37.60802399114341	39.0	35.0	41.0	35.0	41.0
33	37.56401502049884	39.0	35.0	41.0	35.0	41.0
34	37.42249058119512	39.0	35.0	41.0	35.0	41.0
35	37.3662703268285	39.0	35.0	41.0	35.0	41.0
36	37.26922967255164	39.0	35.0	41.0	34.0	41.0
37	37.212915199143104	39.0	35.0	41.0	34.0	41.0
38	37.212202357707554	39.0	35.0	41.0	34.0	41.0
39	37.099051486987236	38.0	35.0	41.0	34.0	41.0
40	37.063328833134356	38.0	35.0	41.0	34.0	41.0
41	37.03895957383239	38.0	35.0	41.0	34.0	41.0
42	36.93664017375975	38.0	35.0	41.0	34.0	41.0
43	36.94108954404182	38.0	35.0	41.0	34.0	41.0
44	36.87144431592637	38.0	35.0	41.0	34.0	41.0
45	36.850890927822014	37.0	35.0	41.0	34.0	41.0
46	36.79163086962936	37.0	35.0	41.0	34.0	41.0
47	36.74097325790851	37.0	35.0	41.0	34.0	41.0
48	36.72512090410609	37.0	35.0	41.0	34.0	41.0
49	36.62858357786894	36.0	35.0	41.0	34.0	41.0
50	36.53422816628669	36.0	35.0	40.0	34.0	41.0
51	36.45148029272369	36.0	35.0	40.0	34.0	41.0
52	36.191919229486594	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	1.0
15	1.0
16	17.0
17	32.0
18	61.0
19	114.0
20	240.0
21	404.0
22	685.0
23	1091.0
24	1729.0
25	2753.0
26	3826.0
27	4384.0
28	4371.0
29	4855.0
30	5770.0
31	7272.0
32	9538.0
33	14095.0
34	49614.0
35	213244.0
36	28417.0
37	41667.0
38	76393.0
39	333838.0
40	2216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.19547599831893	22.27053014327493	24.910522903285393	19.62347095512074
2	25.931559783841685	23.749769101361093	27.56985536137342	22.74881575342381
3	25.647291016586273	25.180286896982633	26.22289497924082	22.949527107190278
4	25.758122363261513	27.092933447883855	23.996846141544275	23.152098047310357
5	24.437072217655906	35.182010113670316	21.339373269809865	19.041544398863916
6	89.22344913597419	2.5090778807162133	5.898855858502835	2.368617124806758
7	90.19328540559438	2.205345442959668	4.409947051377892	3.1914221000680607
8	82.91746783845402	3.8270287157324723	8.472151454630431	4.783351991183081
9	57.432456724326244	16.60709791713931	12.909124494347477	13.051320864186971
10	49.69645352087881	18.790252296279217	19.65756337160362	11.855730811238347
11	44.79036883035737	18.56449851295078	21.346811615224308	15.298321041467537
12	20.539751137756916	40.03106748934767	23.658897314881276	15.770284058014136
13	14.76325606132172	42.13252404135224	25.486374810787087	17.61784508653895
14	12.84478280651252	22.83113344267701	47.6526441458362	16.67143960497427
15	14.97884410591708	16.850331812191698	49.575084518199766	18.595739563691453
16	15.885082522243751	18.74289483047391	25.97110698696182	39.40091566032052
17	20.31449324412278	22.878614880905893	33.18593012170373	23.620961753267604
18	27.20835177423134	25.73741896852464	25.068711715765946	21.98551754147807
19	30.504778517066665	22.33226841021483	23.88365931882112	23.279293753897385
20	21.042211370502745	29.285013841521092	27.075949225854202	22.59682556212196
21	21.716745327169424	24.322769643120584	25.70072313114671	28.259761898563283
22	18.671486714495227	31.300805448835963	21.942623082921433	28.085084753747374
23	19.458959549037914	22.99924004904349	38.22764064361523	19.31415975830336
24	17.782976354739652	22.330532796284793	35.65025395750969	24.23623689146586
25	16.411097515468658	34.14857599075662	26.869287195756176	22.57103929801855
26	17.951454878376854	31.044554449308297	26.892594011388105	24.111396660926744
27	18.069104708348675	27.212690809056433	36.736376360442385	17.981828122152507
28	16.363244159969057	24.040608407065932	34.78083535098453	24.815312081980483
29	18.949556860571935	28.41782673862026	29.312659691978116	23.319956708829686
30	30.587964013284886	25.45587759458786	22.032131172741938	21.92402721938532
31	30.692968656052148	25.679771791562683	23.812995037383885	19.814264515001284
32	26.253516167863623	28.094382685515434	23.625052843245548	22.027048303375395
33	21.338877380115566	26.299633909433183	26.409721421566985	25.95176728888426
34	19.670952393349623	25.381990030137697	31.422174451515005	23.524883124997675
35	31.671978885016816	21.932705289035507	25.637125277853194	20.758190548094483
36	22.320243085128144	29.988681317727682	27.4272870742632	20.263788522880972
37	22.27573698506504	29.665361237046433	25.285787429444195	22.77311434844433
38	20.218786533123573	29.25228512169753	27.334927618700494	23.194000726478404
39	25.692293006343668	24.861677768397197	24.940152312519604	24.50587691273953
40	16.79640380793696	22.330780741131942	29.497130658256378	31.37568479267472
41	22.75972532669833	26.000612423772456	23.68009659931245	27.559565650216765
42	23.644516513746684	24.817543585604817	25.765312763828813	25.772627136819686
43	23.224249997830483	28.356584361374654	26.701056616966124	21.718109023828738
44	18.04567392029317	33.72496221940391	22.929567546994846	25.299796313308065
45	22.96118051500624	29.332371307326397	26.033217171172446	21.673231006494913
46	23.43983804242584	25.199998512330918	27.573202616809915	23.786960828433322
47	24.48554543527338	21.557068845605983	30.934218992327345	23.023166726793292
48	20.99485390469744	20.407720506650502	30.583129088765492	28.014296499886566
49	20.353420585125047	28.601925787627803	28.160955876974725	22.88369775027243
50	18.91162129895826	31.052116767146316	25.230495728530144	24.80576620536528
51	20.84732672064426	24.593029526512122	29.083186735942455	25.47645701690116
52	25.216610817089848	22.065479754683366	30.14401876446603	22.573890663760753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	124.0
1	222.0
2	320.0
3	1203.0
4	2086.0
5	1485.0
6	884.0
7	1036.5
8	1189.0
9	1239.5
10	1290.0
11	1231.0
12	1172.0
13	1197.5
14	1191.0
15	1159.0
16	1069.5
17	980.0
18	977.5
19	975.0
20	1233.0
21	1491.0
22	1572.0
23	1653.0
24	1500.0
25	1347.0
26	1935.5
27	2524.0
28	3488.5
29	4453.0
30	5186.0
31	5919.0
32	6688.5
33	7458.0
34	9030.0
35	10602.0
36	12576.0
37	14550.0
38	16707.0
39	21797.5
40	24731.0
41	34849.0
42	44967.0
43	62646.0
44	80325.0
45	87663.0
46	95001.0
47	92980.5
48	90960.0
49	88373.5
50	85787.0
51	79368.0
52	72949.0
53	64041.5
54	55134.0
55	47734.0
56	40334.0
57	35728.5
58	31123.0
59	29539.5
60	27956.0
61	25628.0
62	23300.0
63	21029.0
64	15983.5
65	13209.0
66	10648.5
67	8088.0
68	6349.5
69	4611.0
70	3923.5
71	3236.0
72	2791.0
73	2346.0
74	1916.0
75	1486.0
76	1349.0
77	1212.0
78	886.0
79	560.0
80	359.0
81	158.0
82	122.0
83	86.0
84	66.5
85	47.0
86	25.0
87	3.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	806631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.209103137794084
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40787604200132	15.859048838676223
2	11.948306798748062	5.3072235603080395
3	4.47879480313723	2.984100471476727
4	2.0551142587008098	1.8256897812575006
5	1.1517368891925779	1.278952167984004
6	0.7194046304725038	0.9586398981582286
7	0.46927602652740874	0.7295539771268988
8	0.3796612077474305	0.6745547936225721
9	0.29614795119935916	0.5919462353009679
>10	4.630577140747888	27.22639934153207
>50	2.18234168697822	31.958474127424
>100	0.2746103967981291	9.019467218893332
>500	0.003355727863113594	0.5659137158536077
>1k	0.0027964398859279945	1.0200358723858267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	2262	0.28042562212461464	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT	2079	0.2577386686105543	Illumina Paired End PCR Primer 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	1676	0.2077777819101919	TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1127	0.1397169213680109	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT	1068	0.132402548377139	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	928	0.11504640907676496	No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	853	0.10574847730870744	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2629455104006665	0.0
2	0.0	0.0	0.0	0.9141726514354147	0.0
3	0.0	0.0	0.0	1.495107428303648	0.0
4	0.0	0.0	0.0	1.8391309037217762	0.0
5	0.0	0.0	0.0	3.74471102647927	0.0
6	0.0	0.0	0.0	4.0200537792373465	0.0
7	0.0	0.0	0.0	5.082125531996662	0.0
8	0.0	0.0	0.0	6.410490050593146	0.0
9	0.0	0.0	0.0	7.467478933985924	0.0
10	1.239724235741002E-4	0.0	0.0	8.205982661216838	0.0
11	1.239724235741002E-4	0.0	0.0	8.874937858822683	0.0
12	1.239724235741002E-4	0.0	0.0	9.449302097241489	0.0
13	1.239724235741002E-4	0.0	0.0	9.691420240481706	0.0
14	1.239724235741002E-4	0.0	0.0	9.881717910667952	0.0
15	1.239724235741002E-4	0.0	0.0	10.547945714955166	0.0
16	1.239724235741002E-4	0.0	0.0	11.262026874741982	0.0
17	1.239724235741002E-4	0.0	0.0	12.217234398380425	0.0
18	1.239724235741002E-4	0.0	0.0	12.667254295954407	0.0
19	1.239724235741002E-4	0.0	0.0	13.1885583370835	0.0
20	1.239724235741002E-4	0.0	0.0	13.88677102665283	0.0
21	1.239724235741002E-4	0.0	0.0	14.459523623565174	0.0
22	1.239724235741002E-4	0.0	0.0	15.095750101347456	0.0
23	1.239724235741002E-4	0.0	0.0	15.559902855208888	0.0
24	1.239724235741002E-4	0.0	0.0	15.965540625143342	0.0
25	1.239724235741002E-4	0.0	0.0	16.375889347173615	0.0
26	1.239724235741002E-4	0.0	0.0	16.73491348584421	0.0
27	2.479448471482004E-4	0.0	0.0	17.141667007590833	0.0
28	2.479448471482004E-4	0.0	0.0	17.523006182504762	0.0
29	2.479448471482004E-4	0.0	0.0	18.022119159814093	0.0
30	2.479448471482004E-4	0.0	0.0	18.50015682511582	0.0
31	2.479448471482004E-4	0.0	0.0	18.888810373020625	0.0
32	3.719172707223005E-4	0.0	0.0	19.271513244593873	0.0
33	3.719172707223005E-4	0.0	0.0	19.685457166907792	0.0
34	3.719172707223005E-4	0.0	0.0	20.247548135392762	0.0
35	3.719172707223005E-4	0.0	0.0	20.638805104192624	0.0
36	3.719172707223005E-4	0.0	0.0	21.05324491620084	0.0
37	3.719172707223005E-4	0.0	0.0	21.414748503342917	0.0
38	3.719172707223005E-4	0.0	0.0	21.782450711663696	0.0
39	3.719172707223005E-4	0.0	0.0	22.191435737034656	0.0
40	4.958896942964008E-4	0.0	0.0	22.588395437318923	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	25	3.416243E-5	46.0	36
CGAACGC	20	6.3103304E-4	46.0	24
GCGAATA	20	6.3103304E-4	46.0	39
ATTACGG	20	6.3103304E-4	46.0	1
TAAACGA	20	6.3103304E-4	46.0	20
GAATACG	20	6.3103304E-4	46.0	12
ACGGGTC	40	5.6079443E-9	46.0	4
ATACTCG	20	6.3103304E-4	46.0	43
AATGCGG	40	5.6079443E-9	46.0	1
ACAATCG	25	3.416243E-5	46.0	44
CGGGAAC	80	0.0	46.0	5
CGGTGAT	25	3.416243E-5	46.0	5
GCGATGT	55	1.8189894E-12	46.0	8
GCGATGA	85	0.0	46.0	8
GTATCGG	45	3.092282E-10	46.0	1
ACGTCCG	20	6.3103304E-4	46.0	22
TTCGGGT	25	3.416243E-5	46.0	11
CGATGCG	60	0.0	46.0	9
CACAACG	20	6.3103304E-4	46.0	11
GGTTCGC	20	6.3103304E-4	46.0	7
>>END_MODULE