FastQCFastQC Report
Thu 26 May 2016
SRR1544877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544877_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences534225
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT26570.49735598296597877TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT20780.3889746829519397Illumina Paired End PCR Primer 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT16170.30268145444335254TruSeq Adapter, Index 22 (95% over 23bp)
TGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTTG10330.19336421919603164TruSeq Adapter, Index 22 (96% over 25bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG10270.1922410969160934No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA8560.16023211193785392No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC6800.12728719172633254No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6230.11661753006691937No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT6020.11268660208713557No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT5940.1111891057138846TruSeq Adapter, Index 22 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC5810.10875567410735178TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC5700.10669661659413168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA351.0181975E-746.00000413
ACGGGTA351.0181975E-746.0000044
TCCGTCA351.0181975E-746.00000428
CGCACGA351.0181975E-746.00000439
TATCACG206.308406E-446.031
CGGGTAC405.6024874E-946.05
AAATCGG301.8595183E-646.01
ACGCCTA206.308406E-446.035
TTAATCG206.308406E-446.019
GTCGAAT253.414683E-546.029
CGACGGT253.414683E-546.027
CGTGATT206.308406E-446.016
GGCGTAG206.308406E-446.07
ATAGCGG551.8189894E-1246.01
TCGGGCA206.308406E-446.04
TGAGTCG206.308406E-446.037
TACGGGT405.6024874E-946.03
AGCGGCA206.308406E-446.035
CTACGGT206.308406E-446.036
TCGTCTA206.308406E-446.041
Produced by FastQC (version 0.11.5)