##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544877_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	534225
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.931526978333096	34.0	33.0	34.0	31.0	34.0
2	33.27720716926389	34.0	34.0	34.0	31.0	34.0
3	33.369685057794	34.0	34.0	34.0	31.0	34.0
4	36.609147830970095	37.0	37.0	37.0	35.0	37.0
5	36.594646450465625	37.0	37.0	37.0	35.0	37.0
6	36.55327811315457	37.0	37.0	37.0	35.0	37.0
7	36.45076325518274	37.0	37.0	37.0	35.0	37.0
8	36.49016425663344	37.0	37.0	37.0	35.0	37.0
9	38.297696663390894	39.0	39.0	39.0	37.0	39.0
10	38.09346623613646	39.0	39.0	39.0	35.0	39.0
11	37.2248341054799	39.0	35.0	39.0	35.0	39.0
12	37.15131264916468	39.0	35.0	39.0	35.0	39.0
13	37.15040104824746	39.0	35.0	39.0	35.0	39.0
14	38.303239271842386	40.0	35.0	41.0	35.0	41.0
15	38.3673564509336	40.0	35.0	41.0	35.0	41.0
16	38.38698675651645	40.0	35.0	41.0	35.0	41.0
17	38.47621320604614	40.0	36.0	41.0	35.0	41.0
18	38.46745098039216	40.0	36.0	41.0	35.0	41.0
19	38.367515559923255	40.0	35.0	41.0	35.0	41.0
20	38.285653048809024	40.0	35.0	41.0	35.0	41.0
21	38.22633347372362	40.0	35.0	41.0	35.0	41.0
22	38.21179839955075	40.0	35.0	41.0	35.0	41.0
23	38.18053067527727	40.0	35.0	41.0	35.0	41.0
24	38.111400627076605	40.0	35.0	41.0	35.0	41.0
25	38.06703542514858	40.0	35.0	41.0	35.0	41.0
26	38.001626655435444	40.0	35.0	41.0	35.0	41.0
27	37.99746174364734	40.0	35.0	41.0	35.0	41.0
28	38.06412092283214	40.0	35.0	41.0	35.0	41.0
29	38.087923627684965	40.0	35.0	41.0	35.0	41.0
30	38.008400954653936	40.0	35.0	41.0	35.0	41.0
31	37.94151340727222	40.0	35.0	41.0	35.0	41.0
32	37.89621601385184	40.0	35.0	41.0	35.0	41.0
33	37.86980204969816	40.0	35.0	41.0	35.0	41.0
34	37.70945949740278	40.0	35.0	41.0	35.0	41.0
35	37.677012494735365	39.0	35.0	41.0	35.0	41.0
36	37.60494922551359	39.0	35.0	41.0	35.0	41.0
37	37.54461135289438	39.0	35.0	41.0	35.0	41.0
38	37.52322523281389	39.0	35.0	41.0	35.0	41.0
39	37.48250081894333	39.0	35.0	41.0	35.0	41.0
40	37.46973466236136	39.0	35.0	41.0	35.0	41.0
41	37.449273246291355	39.0	35.0	41.0	35.0	41.0
42	37.395095699377606	39.0	35.0	41.0	35.0	41.0
43	37.33229444522439	39.0	35.0	41.0	35.0	41.0
44	37.172889700032755	39.0	35.0	41.0	34.0	41.0
45	37.156153306191214	38.0	35.0	41.0	34.0	41.0
46	37.084234170995366	38.0	35.0	41.0	34.0	41.0
47	37.034206560905986	38.0	35.0	41.0	34.0	41.0
48	36.991514811175065	37.0	35.0	41.0	34.0	41.0
49	36.9138640086106	37.0	35.0	41.0	34.0	41.0
50	36.82476859001357	37.0	35.0	41.0	34.0	41.0
51	36.74206186531892	37.0	35.0	40.0	34.0	41.0
52	36.31282137676073	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	9.0
15	5.0
16	17.0
17	31.0
18	61.0
19	81.0
20	157.0
21	252.0
22	366.0
23	565.0
24	900.0
25	1483.0
26	2015.0
27	2385.0
28	2441.0
29	2621.0
30	3213.0
31	3907.0
32	5256.0
33	8002.0
34	29114.0
35	137151.0
36	17941.0
37	27175.0
38	52122.0
39	235961.0
40	991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.17071458655061	22.36248771585006	23.847629744021713	21.619167953577612
2	25.245168234358182	24.231176002620618	27.58687818802939	22.936777574991808
3	25.505919790350507	25.260517572184003	26.183911273339884	23.049651364125605
4	25.63882259347653	26.82558846927793	24.19074360054284	23.3448453367027
5	24.348355093827507	34.5987177687304	21.34681080069259	19.706116336749496
6	87.21624783564977	2.7164584210772613	6.913004820066451	3.154288923206514
7	88.59132388038748	2.2951003790537694	5.1251813374514485	3.9883944031073053
8	80.17838925546353	4.198979830595722	9.772286957742523	5.850343956198231
9	55.75572090411344	16.420047732696897	13.761430109036453	14.062801254153213
10	47.761523702559785	18.601712761476904	21.310870887734566	12.325892648228741
11	42.90439421592026	18.40853572932753	22.03659506762132	16.650474987130888
12	19.932238289110394	38.39786606766812	24.976742009452945	16.693153633768542
13	14.749216154242125	40.68229678506247	26.260657962468997	18.307829098226403
14	12.604988534793392	22.964481257896953	46.41115634798072	18.019373859328937
15	14.541812906546866	17.22532640741261	48.88651785296457	19.34634283307595
16	15.458655061069773	19.75178997613365	26.1915859422528	38.59796902054378
17	19.46333473723618	23.012213954794326	33.319668678927414	24.20478262904207
18	26.270953250035095	25.897515091955636	25.29683185923534	22.534699798773925
19	29.17927839393514	22.481351490476857	23.861668772520943	24.47770134306706
20	20.307735504703075	29.915485048434647	27.066123824231365	22.710655622630917
21	21.41344938930226	24.37194066170621	25.69722495203332	28.51738499695821
22	18.46544059151107	31.539145491132015	21.824886517852963	28.170527399503953
23	18.857035893116198	23.2375871589686	37.89976133651551	20.00561561139969
24	18.277317609621413	22.57981187701811	34.71477373765735	24.42809677570312
25	16.440825494875753	33.591089896579156	26.69549347185175	23.27259113669334
26	18.10828770649071	29.66109785202864	26.40891010342084	25.82170433805981
27	19.153914549113203	26.66835135008657	35.719968178202066	18.457765922598156
28	17.06677897889466	23.936730778230146	33.579858673779775	25.416631569095415
29	19.300856380738455	27.640039309279796	28.388038747718657	24.67106556226309
30	30.592353409144085	25.258084140577473	21.534372221442275	22.615190228836166
31	30.90476859001357	25.28073377322289	23.08072441387056	20.733773222892975
32	25.61935514062427	28.09190883990828	23.809069212410503	22.47966680705695
33	22.00159108989658	25.332023024006737	26.338714960924705	26.327670925171976
34	20.146193083438625	24.6551546632973	31.74916935748046	23.44948289578361
35	31.315269783330997	21.691796527680285	25.876737329776777	21.11619635921194
36	22.77486077963405	29.67962936964762	26.785904815386775	20.759605035331553
37	22.572885956291824	28.922083391829283	26.38888108942861	22.11614956245028
38	20.116804717113578	28.854134493893024	26.964481257896956	24.06457953109645
39	25.688988721980436	24.36950723009968	25.379194159764147	24.56230988815574
40	17.008189433291214	21.826384014226218	29.554775609527823	31.61065094295475
41	22.611259300856382	26.15901539613459	23.53820955589873	27.6915157471103
42	23.054705414385325	24.693340820815198	26.19139875520614	26.060555009593333
43	24.229865693294027	27.43862604707754	26.37484206092938	21.95666619869905
44	18.17118255416725	31.99232533108709	23.744489681314054	26.092002433431606
45	22.695680658898404	28.08685478964856	26.445411577518836	22.772052973934205
46	23.932612663203706	23.748982170433806	28.049043006223968	24.26936216013852
47	23.582572885956292	20.694089569001825	31.792035191164768	23.93130235387711
48	20.724039496466844	19.63367494969348	30.527586691000984	29.11469886283869
49	20.387289999532033	27.019139875520615	29.004632879404745	23.588937245542606
50	19.02849922785343	29.60756235668492	26.235949272310354	25.127989143151293
51	20.57560016846834	23.41485329215218	29.275305348869857	26.734241190509618
52	24.904487809443587	21.56357340072067	31.033927652206465	22.498011137629277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	116.5
2	156.0
3	582.0
4	1008.0
5	754.5
6	501.0
7	594.0
8	687.0
9	657.0
10	627.0
11	636.0
12	645.0
13	694.5
14	697.0
15	650.0
16	612.0
17	574.0
18	568.5
19	563.0
20	716.0
21	869.0
22	912.0
23	955.0
24	912.0
25	869.0
26	1163.5
27	1458.0
28	2131.0
29	2804.0
30	3200.5
31	3597.0
32	4022.0
33	4447.0
34	5435.0
35	6423.0
36	7737.0
37	9051.0
38	10441.5
39	13466.0
40	15100.0
41	21650.0
42	28200.0
43	39069.0
44	49938.0
45	55714.5
46	61491.0
47	63020.5
48	64550.0
49	63440.0
50	62330.0
51	56847.0
52	51364.0
53	44444.5
54	37525.0
55	32154.0
56	26783.0
57	23517.5
58	20252.0
59	19032.5
60	17813.0
61	16359.0
62	14905.0
63	13827.0
64	10625.0
65	8501.0
66	6940.5
67	5380.0
68	4195.0
69	3010.0
70	2505.0
71	2000.0
72	1752.0
73	1504.0
74	1209.5
75	915.0
76	878.5
77	842.0
78	606.5
79	371.0
80	222.5
81	74.0
82	65.0
83	56.0
84	44.0
85	32.0
86	17.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	534225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.280875619279843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.54941072655234	19.066620395565685
2	11.101251707817243	5.835012307029257
3	3.7474387153915307	2.9545791231023593
4	1.7295808343924048	1.8181959512862813
5	0.9696322592982393	1.2741392401529161
6	0.714026130089198	1.1259139148273964
7	0.526971189113575	0.9694484993226501
8	0.4287580956915748	0.9014510546903649
9	0.37650977781173467	0.8905505976101599
>10	7.176361492652051	48.72866661884403
>50	0.5657634289733016	9.688347742170784
>100	0.10572346905056645	4.321889961874852
>500	0.005000434346986251	0.8459422339693361
>1k	0.0035717388192758933	1.5792423595539402
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	2657	0.49735598296597877	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT	2078	0.3889746829519397	Illumina Paired End PCR Primer 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	1617	0.30268145444335254	TruSeq Adapter, Index 22 (95% over 23bp)
TGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTTG	1033	0.19336421919603164	TruSeq Adapter, Index 22 (96% over 25bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG	1027	0.1922410969160934	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	856	0.16023211193785392	No Hit
ACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	680	0.12728719172633254	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	623	0.11661753006691937	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT	602	0.11268660208713557	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	594	0.1111891057138846	TruSeq Adapter, Index 22 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	581	0.10875567410735178	TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	570	0.10669661659413168	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40039309279797836	0.0
2	0.0	0.0	0.0	1.4171931302353877	0.0
3	0.0	0.0	0.0	2.200196546398989	0.0
4	0.0	0.0	0.0	2.6876316158921805	0.0
5	0.0	0.0	0.0	5.285787823482615	0.0
6	0.0	0.0	0.0	5.667462211614956	0.0
7	0.0	0.0	0.0	7.084655341850344	0.0
8	0.0	0.0	0.0	8.76709251719781	0.0
9	0.0	0.0	0.0	10.128503907529598	0.0
10	0.0	0.0	0.0	11.067621320604614	0.0
11	0.0	0.0	0.0	11.905470541438532	0.0
12	0.0	0.0	0.0	12.671814310449717	0.0
13	0.0	0.0	0.0	12.982357620852637	0.0
14	0.0	0.0	0.0	13.238616687725209	0.0
15	0.0	0.0	0.0	14.052318779540455	0.0
16	0.0	0.0	0.0	14.885862698301278	0.0
17	0.0	0.0	0.0	15.94889793626281	0.0
18	0.0	0.0	0.0	16.453367027001732	0.0
19	0.0	0.0	0.0	17.048621835368994	0.0
20	1.8718704665637137E-4	0.0	0.0	17.850156769151575	0.0
21	1.8718704665637137E-4	0.0	0.0	18.493893022602837	0.0
22	1.8718704665637137E-4	0.0	0.0	19.229163741869062	0.0
23	1.8718704665637137E-4	0.0	0.0	19.734194393747952	0.0
24	1.8718704665637137E-4	0.0	0.0	20.173709579297114	0.0
25	1.8718704665637137E-4	0.0	0.0	20.642987505264635	0.0
26	1.8718704665637137E-4	0.0	0.0	21.038326547802892	0.0
27	1.8718704665637137E-4	0.0	0.0	21.46661051055267	0.0
28	1.8718704665637137E-4	0.0	0.0	21.878047639103375	0.0
29	1.8718704665637137E-4	0.0	0.0	22.41527446300716	0.0
30	1.8718704665637137E-4	0.0	0.0	22.924610416959148	0.0
31	1.8718704665637137E-4	0.0	0.0	23.34428377556273	0.0
32	1.8718704665637137E-4	0.0	0.0	23.76171088960644	0.0
33	1.8718704665637137E-4	0.0	0.0	24.183817679816556	0.0
34	1.8718704665637137E-4	0.0	0.0	24.77008750994431	0.0
35	1.8718704665637137E-4	0.0	0.0	25.172352473208853	0.0
36	1.8718704665637137E-4	0.0	0.0	25.592774579999062	0.0
37	3.7437409331274275E-4	0.0	0.0	25.954232767092517	0.0
38	3.7437409331274275E-4	0.0	0.0	26.334035284758293	0.0
39	3.7437409331274275E-4	0.0	0.0	26.76662454958117	0.0
40	3.7437409331274275E-4	0.0	0.0	27.17319481491881	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCA	35	1.0181975E-7	46.000004	13
ACGGGTA	35	1.0181975E-7	46.000004	4
TCCGTCA	35	1.0181975E-7	46.000004	28
CGCACGA	35	1.0181975E-7	46.000004	39
TATCACG	20	6.308406E-4	46.0	31
CGGGTAC	40	5.6024874E-9	46.0	5
AAATCGG	30	1.8595183E-6	46.0	1
ACGCCTA	20	6.308406E-4	46.0	35
TTAATCG	20	6.308406E-4	46.0	19
GTCGAAT	25	3.414683E-5	46.0	29
CGACGGT	25	3.414683E-5	46.0	27
CGTGATT	20	6.308406E-4	46.0	16
GGCGTAG	20	6.308406E-4	46.0	7
ATAGCGG	55	1.8189894E-12	46.0	1
TCGGGCA	20	6.308406E-4	46.0	4
TGAGTCG	20	6.308406E-4	46.0	37
TACGGGT	40	5.6024874E-9	46.0	3
AGCGGCA	20	6.308406E-4	46.0	35
CTACGGT	20	6.308406E-4	46.0	36
TCGTCTA	20	6.308406E-4	46.0	41
>>END_MODULE