FastQCFastQC Report
Thu 26 May 2016
SRR1544876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544876_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2231562
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT90200.40420118284860557Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT87610.3925949626315558No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC74060.33187516188212557No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT59660.26734636994177174No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49400.22136960568426958No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA29050.13017787540745002No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27710.12417311282411155No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC24070.10786166819474431No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC23730.10633807171837484No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT23600.10575552012446886No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23580.10566589680232948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGT206.3127396E-446.046
TACGGAC206.3127396E-446.024
TCTAGCG206.3127396E-446.014
TACTACG206.3127396E-446.038
GTAACGA253.4181976E-546.022
ATCGATA253.4181976E-546.014
CGGTAAT351.0201438E-745.9999965
ATAGCGG3600.044.0833321
CGCACTT21200.042.74528534
ACGTAGG2100.042.7142831
CCGCACT21350.042.22950733
CTAACGG2350.042.0851061
ACGGGTA1150.042.04
TAGTAGG6250.041.9521
CATGCGG4100.041.5121961
AGTACGG2050.041.5121961
CAACGCA25600.041.2382816
ACATACG10000.041.17000216
CAACCGG1900.041.1578941
GCGATAT950.041.1578948
Produced by FastQC (version 0.11.5)