##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544876_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2231562
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90999443439169	34.0	33.0	34.0	31.0	34.0
2	33.240162720103676	34.0	33.0	34.0	31.0	34.0
3	33.30603541375951	34.0	34.0	34.0	31.0	34.0
4	36.579832422312265	37.0	37.0	37.0	35.0	37.0
5	36.58791554973602	37.0	37.0	37.0	35.0	37.0
6	36.67260958915773	37.0	37.0	37.0	35.0	37.0
7	36.6432377859096	37.0	37.0	37.0	35.0	37.0
8	36.53647893269378	37.0	37.0	37.0	35.0	37.0
9	38.33844141457867	39.0	39.0	39.0	37.0	39.0
10	38.160761834087516	39.0	39.0	39.0	35.0	39.0
11	37.6665214768848	39.0	37.0	39.0	35.0	39.0
12	37.60976750814004	39.0	37.0	39.0	35.0	39.0
13	37.60287413031769	39.0	37.0	39.0	35.0	39.0
14	38.95935537529318	41.0	37.0	41.0	35.0	41.0
15	39.01785206953694	41.0	38.0	41.0	35.0	41.0
16	38.98905654425017	41.0	37.0	41.0	35.0	41.0
17	39.02312729827807	41.0	37.0	41.0	35.0	41.0
18	38.99797630538609	41.0	37.0	41.0	35.0	41.0
19	38.94265182862945	41.0	37.0	41.0	35.0	41.0
20	38.86132717800357	41.0	36.0	41.0	35.0	41.0
21	38.80452974194757	41.0	36.0	41.0	35.0	41.0
22	38.78211629343034	41.0	35.0	41.0	35.0	41.0
23	38.74984965687711	41.0	35.0	41.0	35.0	41.0
24	38.682143270050304	41.0	35.0	41.0	35.0	41.0
25	38.63178571780663	41.0	35.0	41.0	35.0	41.0
26	38.56806667258181	40.0	35.0	41.0	35.0	41.0
27	38.517853413886776	40.0	36.0	41.0	35.0	41.0
28	38.55414637818712	40.0	36.0	41.0	35.0	41.0
29	38.561683251462426	40.0	36.0	41.0	35.0	41.0
30	38.5099912079521	40.0	35.0	41.0	35.0	41.0
31	38.43127101106759	40.0	35.0	41.0	35.0	41.0
32	38.37217966608143	40.0	35.0	41.0	35.0	41.0
33	38.2575949043764	40.0	35.0	41.0	35.0	41.0
34	38.140840362042375	40.0	35.0	41.0	35.0	41.0
35	38.05311167693302	40.0	35.0	41.0	35.0	41.0
36	38.001631144462934	40.0	35.0	41.0	35.0	41.0
37	37.8946912521364	40.0	35.0	41.0	35.0	41.0
38	37.84746289818522	40.0	35.0	41.0	35.0	41.0
39	37.740932584440856	40.0	35.0	41.0	35.0	41.0
40	37.63055160466077	40.0	35.0	41.0	34.0	41.0
41	37.5728126756057	40.0	35.0	41.0	34.0	41.0
42	37.51313205727647	39.0	35.0	41.0	34.0	41.0
43	37.51251276012049	39.0	35.0	41.0	34.0	41.0
44	37.48567057513974	39.0	35.0	41.0	34.0	41.0
45	37.46316033343461	39.0	35.0	41.0	35.0	41.0
46	37.42406574408419	39.0	35.0	41.0	35.0	41.0
47	37.31828692189596	39.0	35.0	41.0	34.0	41.0
48	37.26648688228246	39.0	35.0	41.0	34.0	41.0
49	37.21388023276969	39.0	35.0	41.0	34.0	41.0
50	37.13831880987398	39.0	35.0	41.0	34.0	41.0
51	37.05922891678564	38.0	35.0	41.0	34.0	41.0
52	36.793211212594585	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	5.0
13	8.0
14	11.0
15	19.0
16	45.0
17	63.0
18	147.0
19	270.0
20	472.0
21	767.0
22	1349.0
23	2262.0
24	3878.0
25	6219.0
26	9066.0
27	11247.0
28	11588.0
29	12864.0
30	14919.0
31	18885.0
32	24031.0
33	33788.0
34	102994.0
35	359571.0
36	94712.0
37	152704.0
38	248751.0
39	1108369.0
40	12554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.52604050436421	21.487908469493565	24.11678456614694	19.869266459995284
2	27.990304549010965	24.013180005753817	25.868382774038988	22.12813267119623
3	28.691203739801985	23.893756929003093	25.01364515079572	22.4013941803992
4	26.784512372947738	26.292838827691096	22.56164068038441	24.361008118976752
5	25.23505956814106	35.87393045767942	19.701536412611436	19.189473561568086
6	87.32874103430692	3.004756309705937	6.991963476703762	2.67453917928339
7	87.65671758167596	2.9724022904136205	5.517435769205606	3.8534443587047997
8	77.34004253522869	5.7571781559284485	10.350687097199183	6.552092211643683
9	49.154493578937085	22.708802175337276	13.97406838797219	14.162635857753447
10	37.968651554382085	22.696344533559902	23.58455646762223	15.75044744443578
11	36.08925048911928	18.45639959812902	25.311149768637392	20.1432001441143
12	22.7018115562104	28.66194172512348	28.259577820378727	20.376668898287388
13	17.252803193458213	30.919329151509125	29.404381325726103	22.42348632930656
14	15.946812143243164	26.004072483758012	37.52210335182262	20.5270120211762
15	18.84872569079416	19.550610738128718	40.472547928312096	21.12811564276502
16	19.815133973423098	22.259430838130424	27.295723802430764	30.629711386015714
17	21.23862119896288	24.282542900443723	28.96742281863556	25.51141308195784
18	24.798728424305487	26.81372957596518	26.575241915752283	21.812300083977053
19	28.103812486500484	22.739542974831082	25.34798495403668	23.80865958463175
20	24.366699199932604	26.621890854925834	27.06951453735097	21.941895407790597
21	22.424741055816508	23.715809822895352	26.158583091126303	27.70086603016183
22	21.234498526144467	27.280129344378512	24.479310904200734	27.006061225276284
23	22.176350018507215	22.26713844383441	33.319576153384936	22.236935384273437
24	22.545194800771835	22.21874184987914	30.03636018179195	25.199703167557075
25	19.950689248158913	28.609153588383386	26.764884865399214	24.67527229805849
26	20.03215684798361	27.457673145536628	28.261773591771146	24.24839641470862
27	22.33489367537178	24.929175169679354	32.22989099115328	20.506040163795582
28	19.486081946188367	24.895611235538155	29.34993515752643	26.268371660747047
29	20.706975652032074	25.02233861304324	30.040258796305007	24.230426938619676
30	27.11159268709541	25.2524464926361	23.271636638372584	24.36432418189591
31	25.027671200710532	27.023134468143837	26.00725411169396	21.941940219451666
32	24.786808522460948	26.54965445728149	24.53496698724929	24.12857003300827
33	20.979789044624347	27.256110294045154	27.248089006713684	24.51601165461681
34	22.03120504830249	25.112902980065083	27.653679351055448	25.202212620576976
35	25.18142000984064	26.237093121320402	25.043400093745994	23.53808677509296
36	23.942377581263706	28.72113792939654	25.377381403698397	21.959103085641356
37	22.13539216028952	29.27411382699652	26.065509271084558	22.524984741629407
38	21.64949931931087	27.689483868250132	24.988819490563113	25.67219732187589
39	25.957871661195163	24.597389631119366	27.01605422569483	22.42868448199064
40	19.23199982792322	22.988068447123585	31.328145935447903	26.451785789505287
41	21.77071486250438	26.57909571860428	25.284486830300928	26.365702588590413
42	23.63098134849043	22.779649411488457	27.357429459723726	26.231939780297388
43	22.850631082622844	25.937616790391665	27.88454902888649	23.327203098099
44	21.187132600393806	27.40613973530648	25.626220557618385	25.780507106681327
45	22.157484309196875	26.84801049668349	26.16884496151126	24.82566023260837
46	23.679422754106767	24.656137718781732	27.542949736552245	24.12148979055926
47	22.438901540714532	22.78619191400463	30.75648357518187	24.01842297009897
48	21.293784353739667	22.444413375026105	28.37662587909276	27.88517639214147
49	20.7335489670464	25.093858024110467	30.366398065570216	23.806194943272917
50	20.11012913824487	25.958499024450138	28.11895882794204	25.81241300936295
51	21.520979475362996	23.399753177370826	28.042062017546453	27.037205329719722
52	22.478246179133716	21.065200070623177	30.86425562005447	25.592298130188627
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	299.0
1	850.5
2	1402.0
3	5231.5
4	9061.0
5	6045.0
6	3029.0
7	3160.5
8	3292.0
9	3357.5
10	3423.0
11	3278.5
12	3134.0
13	3093.0
14	2949.0
15	2846.0
16	2895.0
17	2944.0
18	3033.0
19	3122.0
20	3442.5
21	3763.0
22	4484.5
23	5206.0
24	5675.5
25	6145.0
26	8057.0
27	9969.0
28	14437.5
29	18906.0
30	20966.0
31	23026.0
32	25318.0
33	27610.0
34	31386.0
35	35162.0
36	39061.0
37	42960.0
38	47822.5
39	61335.0
40	69985.0
41	89260.0
42	108535.0
43	141774.5
44	175014.0
45	206552.5
46	238091.0
47	242224.5
48	246358.0
49	219924.5
50	193491.0
51	174973.0
52	156455.0
53	137160.5
54	117866.0
55	109790.0
56	101714.0
57	97453.5
58	93193.0
59	96428.0
60	99663.0
61	100908.5
62	102154.0
63	97056.0
64	76507.5
65	61057.0
66	50398.5
67	39740.0
68	32779.5
69	25819.0
70	21380.5
71	16942.0
72	14597.5
73	12253.0
74	10736.0
75	9219.0
76	7548.0
77	5877.0
78	4291.0
79	2705.0
80	2149.5
81	1594.0
82	1106.5
83	619.0
84	383.5
85	148.0
86	86.5
87	25.0
88	19.0
89	22.0
90	31.0
91	17.0
92	3.0
93	2.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2231562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.677264950031045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.56665758825883	14.177709456904845
2	12.78895765387998	5.288813316880075
3	5.214284026058561	3.234513969945823
4	2.528111821379796	2.0909775181590247
5	1.4600542889851742	1.5094964687387822
6	0.9370454111969311	1.162532174251784
7	0.6466212143373862	0.9359250719815464
8	0.4686436724319688	0.7752215505625102
9	0.3636022535586188	0.6766470119933935
>10	4.505140500409896	24.614853795020046
>50	2.213399147567277	30.853652276734028
>100	0.2909206616425119	9.580728752022468
>500	0.00958838753807077	1.3842266500766152
>1k	0.00610170116059049	2.3109645899244584
>5k	8.7167159437007E-4	1.4037373968045832
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT	9020	0.40420118284860557	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	8761	0.3925949626315558	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7406	0.33187516188212557	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	5966	0.26734636994177174	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4940	0.22136960568426958	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2905	0.13017787540745002	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2771	0.12417311282411155	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2407	0.10786166819474431	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2373	0.10633807171837484	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	2360	0.10575552012446886	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2358	0.10566589680232948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4133427617068224	0.0
2	0.0	0.0	0.0	1.270858707936414	0.0
3	0.0	0.0	0.0	1.9702791139121387	0.0
4	0.0	0.0	0.0	2.4398605102614224	0.0
5	0.0	0.0	0.0	4.652749957204864	0.0
6	0.0	0.0	0.0	5.0078375595210884	0.0
7	0.0	0.0	0.0	6.217393915114167	0.0
8	0.0	0.0	0.0	7.808880058004214	0.0
9	0.0	0.0	0.0	8.898834090202289	0.0
10	0.0	0.0	0.0	9.812364612768993	0.0
11	0.0	0.0	0.0	10.672614070323837	0.0
12	0.0	0.0	0.0	11.43562222335745	0.0
13	0.0	0.0	0.0	11.770051649920548	0.0
14	0.0	0.0	0.0	12.004909565586795	0.0
15	0.0	0.0	0.0	12.777641849072532	0.0
16	0.0	0.0	0.0	13.600921686244881	0.0
17	0.0	0.0	0.0	14.627512029690415	0.0
18	0.0	0.0	0.0	15.177485545998723	0.0
19	0.0	0.0	0.0	15.80968846036991	0.0
20	4.48116610696902E-5	0.0	0.0	16.575609371373055	0.0
21	8.96233221393804E-5	0.0	0.0	17.30406773372194	0.0
22	8.96233221393804E-5	0.0	0.0	18.079175035244372	0.0
23	8.96233221393804E-5	0.0	0.0	18.667059216817638	0.0
24	1.344349832090706E-4	0.0	0.0	19.174820148398297	0.0
25	1.344349832090706E-4	0.0	0.0	19.662191774192248	0.0
26	1.344349832090706E-4	0.0	0.0	20.118195237237416	0.0
27	1.344349832090706E-4	0.0	0.0	20.589613911690556	0.0
28	1.792466442787608E-4	0.0	0.0	21.05619292674817	0.0
29	1.792466442787608E-4	0.0	0.0	21.62171608944766	0.0
30	1.792466442787608E-4	0.0	0.0	22.169314587719274	0.0
31	1.792466442787608E-4	0.0	0.0	22.70199080285468	0.0
32	1.792466442787608E-4	0.0	0.0	23.197428527641176	0.0
33	1.792466442787608E-4	0.0	0.0	23.6601537398468	0.0
34	2.24058305348451E-4	0.0	0.0	24.25579033878512	0.0
35	2.24058305348451E-4	0.0	0.0	24.748270493941014	0.0
36	2.24058305348451E-4	0.0	0.0	25.28990904129036	0.0
37	2.24058305348451E-4	0.0	0.0	25.76123809242136	0.0
38	2.24058305348451E-4	0.0	0.0	26.270925925428017	0.0
39	2.24058305348451E-4	0.0	0.0	26.79593934652051	0.0
40	2.24058305348451E-4	0.0	0.0	27.33690571895381	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGT	20	6.3127396E-4	46.0	46
TACGGAC	20	6.3127396E-4	46.0	24
TCTAGCG	20	6.3127396E-4	46.0	14
TACTACG	20	6.3127396E-4	46.0	38
GTAACGA	25	3.4181976E-5	46.0	22
ATCGATA	25	3.4181976E-5	46.0	14
CGGTAAT	35	1.0201438E-7	45.999996	5
ATAGCGG	360	0.0	44.083332	1
CGCACTT	2120	0.0	42.745285	34
ACGTAGG	210	0.0	42.714283	1
CCGCACT	2135	0.0	42.229507	33
CTAACGG	235	0.0	42.085106	1
ACGGGTA	115	0.0	42.0	4
TAGTAGG	625	0.0	41.952	1
CATGCGG	410	0.0	41.512196	1
AGTACGG	205	0.0	41.512196	1
CAACGCA	2560	0.0	41.23828	16
ACATACG	1000	0.0	41.170002	16
CAACCGG	190	0.0	41.157894	1
GCGATAT	95	0.0	41.157894	8
>>END_MODULE