FastQCFastQC Report
Thu 26 May 2016
SRR1544875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544875_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1714511
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT118370.6904009364769312No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT110950.6471232905475672Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA105720.6166189659908861No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT72190.42105299995158973No Hit
TGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTTG56300.3283735129141779Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC53770.3136171188169688No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT26400.15397976449261627No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC25760.15024692171703768No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC25350.14785556931393268No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC25050.1461057992628802No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21830.1273249340482505No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC20930.12207562389509312No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC19840.11571812604293585No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT18940.11046881588977848No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17180.10020349825693739No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG206.312328E-446.011
ATGTACG206.312328E-446.045
CGTAAAC206.312328E-446.012
ATAGTAC351.0199619E-745.99999643
ACGTAGG1700.044.647061
TAACGGG4300.043.8604662
CTAACGG1650.043.212121
CATGCGG4150.042.6746981
GTCCCCC14950.042.3076939
CTAGCGG3650.042.2191771
ATGTAGG2300.042.01
CGCACTT16500.041.81818434
ATAGCGG1650.041.818181
TCGGCGT14850.041.66334
CAACGCA19500.041.635916
CAACCGG1550.041.5483861
AAGCTTA18800.041.47340422
TAGTAGG4500.041.41
ACCGGTA508.731149E-1041.39999841
GACGTAG508.731149E-1041.3999989
Produced by FastQC (version 0.11.5)