##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544875_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1714511
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88614362929138	34.0	33.0	34.0	31.0	34.0
2	33.25091119275409	34.0	33.0	34.0	31.0	34.0
3	33.351502556705675	34.0	34.0	34.0	31.0	34.0
4	36.56423493345916	37.0	37.0	37.0	35.0	37.0
5	36.59048556702174	37.0	37.0	37.0	35.0	37.0
6	36.511325386655436	37.0	37.0	37.0	35.0	37.0
7	36.383745569436414	37.0	37.0	37.0	35.0	37.0
8	36.42498065045952	37.0	37.0	37.0	35.0	37.0
9	38.28226648881226	39.0	39.0	39.0	37.0	39.0
10	38.18131058943337	39.0	39.0	39.0	35.0	39.0
11	37.772466901641344	39.0	37.0	39.0	35.0	39.0
12	37.75567902451486	39.0	37.0	39.0	35.0	39.0
13	37.753961333581415	39.0	37.0	39.0	35.0	39.0
14	39.09836507318997	41.0	38.0	41.0	35.0	41.0
15	39.149966375252184	41.0	38.0	41.0	35.0	41.0
16	39.13866519374912	41.0	38.0	41.0	35.0	41.0
17	39.18117527388276	41.0	38.0	41.0	35.0	41.0
18	39.17599945407174	41.0	38.0	41.0	35.0	41.0
19	39.13250192037263	41.0	38.0	41.0	35.0	41.0
20	39.06354464917402	41.0	38.0	41.0	35.0	41.0
21	39.00725046383488	41.0	38.0	41.0	35.0	41.0
22	38.994705779082196	41.0	37.0	41.0	35.0	41.0
23	38.97620662684579	41.0	37.0	41.0	35.0	41.0
24	38.8886918777424	41.0	37.0	41.0	35.0	41.0
25	38.83167912016896	41.0	37.0	41.0	35.0	41.0
26	38.7565999868184	41.0	36.0	41.0	35.0	41.0
27	38.728368030301354	41.0	36.0	41.0	35.0	41.0
28	38.79024339884667	41.0	36.0	41.0	35.0	41.0
29	38.83256858661158	41.0	36.0	41.0	35.0	41.0
30	38.79759709911456	41.0	36.0	41.0	35.0	41.0
31	38.76059354533158	41.0	36.0	41.0	35.0	41.0
32	38.72639195665703	41.0	36.0	41.0	35.0	41.0
33	38.63511753497061	41.0	36.0	41.0	35.0	41.0
34	38.540292829850614	41.0	36.0	41.0	35.0	41.0
35	38.449846632655024	40.0	36.0	41.0	35.0	41.0
36	38.42837053830509	40.0	35.0	41.0	35.0	41.0
37	38.34174175610422	40.0	35.0	41.0	35.0	41.0
38	38.27952226611553	40.0	35.0	41.0	35.0	41.0
39	38.23543564316589	40.0	35.0	41.0	35.0	41.0
40	38.158120886946776	40.0	35.0	41.0	35.0	41.0
41	38.11068928691621	40.0	35.0	41.0	35.0	41.0
42	38.07390445438962	40.0	35.0	41.0	35.0	41.0
43	37.99722428144235	40.0	35.0	41.0	35.0	41.0
44	37.870281963778595	40.0	35.0	41.0	35.0	41.0
45	37.85637712443956	40.0	35.0	41.0	35.0	41.0
46	37.812608376382535	40.0	35.0	41.0	35.0	41.0
47	37.715592375901934	39.0	35.0	41.0	35.0	41.0
48	37.6360046683865	39.0	35.0	41.0	35.0	41.0
49	37.59987250008895	39.0	35.0	41.0	35.0	41.0
50	37.539255216210336	39.0	35.0	41.0	35.0	41.0
51	37.45959401835275	39.0	35.0	41.0	35.0	41.0
52	37.02133261320575	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	6.0
14	8.0
15	25.0
16	42.0
17	59.0
18	113.0
19	201.0
20	299.0
21	524.0
22	798.0
23	1374.0
24	2228.0
25	3678.0
26	5386.0
27	6426.0
28	6849.0
29	7415.0
30	8851.0
31	10986.0
32	15031.0
33	21536.0
34	68625.0
35	256571.0
36	67705.0
37	114570.0
38	190846.0
39	914709.0
40	9647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.37878847088179	21.175192226821526	23.11049622895391	22.335523073342777
2	27.70714215306872	24.27321842787827	25.648770990678976	22.370868428374038
3	28.984999221352325	23.720174440408957	24.840610529766213	22.4542158084725
4	26.69898297532066	25.609517815867033	23.17383790480201	24.5176613040103
5	24.92098330077789	34.80065161436701	19.889869472986756	20.38849561186834
6	84.85614848781957	3.0133956562541737	8.302133961228595	3.828321894697672
7	85.47142596343798	2.971459500697283	6.511885896328458	5.045228639536288
8	73.40472006303838	5.8018875352797386	12.320714186144038	8.472678215537842
9	47.602319261877	21.59233740699243	15.153650224466334	15.651693106664233
10	35.7153147457205	21.95739776531034	26.091521139263612	16.235766349705543
11	33.489957194791984	18.24940172445671	26.026721321706304	22.233919759044998
12	21.89463934614593	26.79317892973565	29.74264965345804	21.56953207066038
13	16.863467192686425	29.324862890935083	30.441449486180023	23.370220430198465
14	15.490014353946984	25.837571179187535	36.31822717964481	22.354187287220668
15	17.99591836972758	19.4831645874538	40.04774539212638	22.47317165069224
16	18.84391526213597	23.59413267106481	27.40711491498159	30.15483715181763
17	19.933555398594702	24.74209847589196	29.145978066049157	26.178368059464184
18	23.418572409275882	27.045204142755573	27.186352260207137	22.34987118776141
19	26.30510973682875	22.517965763999182	25.82479785781485	25.352126641357213
20	23.093756762132177	27.29180506861723	27.539164228167685	22.07527394108291
21	21.51248956699607	23.489496422011875	26.37638370357496	28.6216303074171
22	20.411300948200388	27.19463450511545	24.645044563726916	27.74901998295724
23	20.85142644170845	22.171103014212214	33.79085931790463	23.186611226174694
24	22.825225384963993	22.144039904089272	29.35414237645603	25.67659233449071
25	19.944170670237753	27.874245192944226	26.841822537154908	25.339761599663113
26	19.614164038609257	26.34366300362028	28.48211530867985	25.560057649090616
27	23.751145370312585	23.759660917894372	31.53348097504186	20.955712736751178
28	19.765577473693664	24.452686509447883	28.71815928856683	27.063576728291622
29	20.85930040693819	24.13638640988597	29.79082665553035	25.21348652764549
30	26.86836071626254	25.708263172414757	22.46938048224829	24.953995629074413
31	24.2275494295458	26.543370092113726	26.255591244383968	22.973489233956503
32	24.060563041007025	26.305401365170596	24.384212174783364	25.249823419039014
33	20.915001420230027	27.25943432267276	27.243978020555133	24.58158623654208
34	22.38049216365483	24.503138212586563	27.851439856612174	25.264929767146434
35	24.24043940225522	26.37393402550348	25.412027102771578	23.97359946946972
36	24.889954045205894	28.26205256192582	24.4755501714483	22.372443221419985
37	22.08483935069533	29.399519746446657	26.58518959633388	21.930451306524134
38	21.393505203524505	27.572584836142784	24.221075280356906	26.812834679975804
39	26.824616464986228	24.00422044536314	26.83412354893028	22.337039540720358
40	19.464966978922853	22.386674684501877	31.54742080978191	26.600937526793356
41	21.95675618295829	26.416745066085902	25.01879544663172	26.607703304324087
42	23.24050414374711	21.56154145409391	28.00921078954874	27.188743612610246
43	23.862255768554416	24.934981461186307	27.552404154887313	23.650358615371964
44	21.394146785876554	25.801059310788908	26.355094834620484	26.449699068714054
45	21.62400824491648	25.387238693715002	26.572007995282622	26.416745066085902
46	24.18817960339712	23.114637351408067	27.707550432747297	24.989632612447515
47	21.43369158903034	22.048560784970174	31.836307845210676	24.681439780788807
48	21.080704644064692	21.055624606666274	28.09722422311668	29.766446526152357
49	20.707828646185416	23.323443244166995	30.995659987016705	24.973068122630885
50	20.23276607732467	23.891651905412097	29.243848537571353	26.631733479691878
51	21.528762428470856	21.696448725030052	28.15315853908199	28.6216303074171
52	21.793444311526727	20.271144367111088	32.68838753440485	25.247023786957328
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	219.0
1	486.0
2	753.0
3	2650.0
4	4547.0
5	3167.5
6	1788.0
7	1827.0
8	1866.0
9	1942.5
10	2019.0
11	2022.0
12	2025.0
13	1954.0
14	1762.0
15	1641.0
16	1797.5
17	1954.0
18	1957.0
19	1960.0
20	2281.0
21	2602.0
22	3046.0
23	3490.0
24	3664.0
25	3838.0
26	5128.0
27	6418.0
28	9058.5
29	11699.0
30	13122.5
31	14546.0
32	16218.0
33	17890.0
34	20325.0
35	22760.0
36	25436.5
37	28113.0
38	31948.0
39	41937.0
40	48091.0
41	63188.5
42	78286.0
43	105550.5
44	132815.0
45	167730.5
46	202646.0
47	207792.0
48	212938.0
49	189123.0
50	165308.0
51	146024.5
52	126741.0
53	109303.0
54	91865.0
55	83991.0
56	76117.0
57	71641.0
58	67165.0
59	69121.5
60	71078.0
61	71688.0
62	72298.0
63	72667.5
64	58638.5
65	44240.0
66	36454.0
67	28668.0
68	23881.5
69	19095.0
70	15755.5
71	12416.0
72	10734.0
73	9052.0
74	7974.0
75	6896.0
76	5625.5
77	4355.0
78	3143.0
79	1931.0
80	1518.0
81	1105.0
82	753.5
83	402.0
84	257.0
85	112.0
86	66.0
87	20.0
88	19.0
89	19.0
90	20.0
91	11.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1714511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.76934625606647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.18096971769717	16.206170772943267
2	12.959180764834647	6.16062509588622
3	5.22176359962276	3.723537212002723
4	2.5387837190482725	2.413809171492903
5	1.433170832072908	1.7032766875817922
6	0.9311820492226573	1.3280153133244126
7	0.644152607003955	1.0717760450337444
8	0.4717918914207699	0.8971347862387836
9	0.3657748945841981	0.7824807109033214
>10	6.3350946828495776	41.004201258671166
>50	0.7413328987397763	11.091723391826477
>100	0.15958932012096427	6.666097247101786
>500	0.009344212367582965	1.5245811024882099
>1k	0.00639340846203045	2.403009617642914
>5k	7.37700976388129E-4	1.065289647828175
>10k+	7.37700976388129E-4	1.9582719390340817
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	11837	0.6904009364769312	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT	11095	0.6471232905475672	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	10572	0.6166189659908861	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	7219	0.42105299995158973	No Hit
TGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTTG	5630	0.3283735129141779	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5377	0.3136171188169688	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	2640	0.15397976449261627	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2576	0.15024692171703768	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2535	0.14785556931393268	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	2505	0.1461057992628802	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2183	0.1273249340482505	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2093	0.12207562389509312	No Hit
AGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1984	0.11571812604293585	No Hit
ACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	1894	0.11046881588977848	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1718	0.10020349825693739	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6593716809049344	0.0
2	0.0	0.0	0.0	2.0567963693437954	0.0
3	0.0	0.0	0.0	3.082628224607483	0.0
4	0.0	0.0	0.0	3.766263383553678	0.0
5	0.0	0.0	0.0	6.956677443305992	0.0
6	0.0	0.0	0.0	7.485457952734045	0.0
7	0.0	0.0	0.0	9.257858363113447	0.0
8	0.0	0.0	0.0	11.448337164357651	0.0
9	0.0	0.0	0.0	12.964571239262973	0.0
10	0.0	0.0	0.0	14.221606043939058	0.0
11	0.0	0.0	0.0	15.365722354653892	0.0
12	0.0	0.0	0.0	16.419200576724208	0.0
13	0.0	0.0	0.0	16.8747823723499	0.0
14	0.0	0.0	0.0	17.194465360677185	0.0
15	0.0	0.0	0.0	18.193584059828137	0.0
16	0.0	0.0	0.0	19.23656366159214	0.0
17	0.0	0.0	0.0	20.46694363582386	0.0
18	0.0	0.0	0.0	21.163352116142736	0.0
19	0.0	0.0	0.0	21.940716624156977	0.0
20	0.0	0.0	0.0	22.875093831418987	0.0
21	0.0	0.0	0.0	23.74537112914411	0.0
22	5.832566836841525E-5	0.0	0.0	24.678173543360177	0.0
23	5.832566836841525E-5	0.0	0.0	25.37627346806174	0.0
24	5.832566836841525E-5	0.0	0.0	25.977202829261522	0.0
25	1.166513367368305E-4	0.0	0.0	26.519164939740836	0.0
26	1.166513367368305E-4	0.0	0.0	27.047828797832153	0.0
27	1.166513367368305E-4	0.0	0.0	27.588624394944098	0.0
28	1.166513367368305E-4	0.0	0.0	28.118046486724204	0.0
29	1.166513367368305E-4	0.0	0.0	28.74452249066935	0.0
30	1.166513367368305E-4	0.0	0.0	29.353850748114187	0.0
31	1.166513367368305E-4	0.0	0.0	29.94066529756881	0.0
32	1.166513367368305E-4	0.0	0.0	30.493942587711597	0.0
33	1.166513367368305E-4	0.0	0.0	30.992743703598286	0.0
34	1.7497700510524575E-4	0.0	0.0	31.64068355350301	0.0
35	1.7497700510524575E-4	0.0	0.0	32.170047319614746	0.0
36	1.7497700510524575E-4	0.0	0.0	32.728107314563744	0.0
37	1.7497700510524575E-4	0.0	0.0	33.23921514647617	0.0
38	1.7497700510524575E-4	0.0	0.0	33.782518747327956	0.0
39	1.7497700510524575E-4	0.0	0.0	34.32401425246032	0.0
40	1.7497700510524575E-4	0.0	0.0	34.869009297694795	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	20	6.312328E-4	46.0	11
ATGTACG	20	6.312328E-4	46.0	45
CGTAAAC	20	6.312328E-4	46.0	12
ATAGTAC	35	1.0199619E-7	45.999996	43
ACGTAGG	170	0.0	44.64706	1
TAACGGG	430	0.0	43.860466	2
CTAACGG	165	0.0	43.21212	1
CATGCGG	415	0.0	42.674698	1
GTCCCCC	1495	0.0	42.307693	9
CTAGCGG	365	0.0	42.219177	1
ATGTAGG	230	0.0	42.0	1
CGCACTT	1650	0.0	41.818184	34
ATAGCGG	165	0.0	41.81818	1
TCGGCGT	1485	0.0	41.6633	4
CAACGCA	1950	0.0	41.6359	16
CAACCGG	155	0.0	41.548386	1
AAGCTTA	1880	0.0	41.473404	22
TAGTAGG	450	0.0	41.4	1
ACCGGTA	50	8.731149E-10	41.399998	41
GACGTAG	50	8.731149E-10	41.399998	9
>>END_MODULE