##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544874_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1875006
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.837294920656255	33.0	31.0	34.0	31.0	34.0
2	33.188546063319265	34.0	33.0	34.0	31.0	34.0
3	33.2557207816935	34.0	34.0	34.0	31.0	34.0
4	36.561876601994875	37.0	37.0	37.0	35.0	37.0
5	36.555515022351926	37.0	37.0	37.0	35.0	37.0
6	36.66322347768487	37.0	37.0	37.0	35.0	37.0
7	36.63780649235256	37.0	37.0	37.0	35.0	37.0
8	36.530960434259946	37.0	37.0	37.0	35.0	37.0
9	38.30224063282998	39.0	39.0	39.0	37.0	39.0
10	38.1343841033042	39.0	39.0	39.0	35.0	39.0
11	37.64237767772477	39.0	37.0	39.0	35.0	39.0
12	37.611180444222576	39.0	37.0	39.0	35.0	39.0
13	37.59320076842421	39.0	37.0	39.0	35.0	39.0
14	38.94955696141772	41.0	38.0	41.0	35.0	41.0
15	39.00216372640941	41.0	38.0	41.0	35.0	41.0
16	38.99334455463076	41.0	38.0	41.0	35.0	41.0
17	39.027818044315595	41.0	38.0	41.0	35.0	41.0
18	39.00294772390062	41.0	37.0	41.0	35.0	41.0
19	38.94051165702936	41.0	37.0	41.0	35.0	41.0
20	38.86523243125622	41.0	36.0	41.0	35.0	41.0
21	38.804287026281514	41.0	36.0	41.0	35.0	41.0
22	38.7688882062244	41.0	36.0	41.0	35.0	41.0
23	38.73013793022529	41.0	36.0	41.0	35.0	41.0
24	38.66619840149845	41.0	36.0	41.0	35.0	41.0
25	38.63970035295887	40.0	36.0	41.0	35.0	41.0
26	38.57321896569931	40.0	35.0	41.0	35.0	41.0
27	38.539928405562435	40.0	36.0	41.0	35.0	41.0
28	38.56289419873856	40.0	36.0	41.0	35.0	41.0
29	38.55666648533391	40.0	36.0	41.0	35.0	41.0
30	38.475434211943856	40.0	36.0	41.0	35.0	41.0
31	38.441374054269694	40.0	35.0	41.0	35.0	41.0
32	38.35692205784942	40.0	35.0	41.0	35.0	41.0
33	38.26031223366752	40.0	35.0	41.0	35.0	41.0
34	38.17998129072654	40.0	35.0	41.0	35.0	41.0
35	38.11489136568096	40.0	35.0	41.0	35.0	41.0
36	38.04693851646341	40.0	35.0	41.0	35.0	41.0
37	37.97681554085693	40.0	35.0	41.0	35.0	41.0
38	37.93712820118976	40.0	35.0	41.0	35.0	41.0
39	37.851984473649686	40.0	35.0	41.0	35.0	41.0
40	37.74990160031488	40.0	35.0	41.0	34.0	41.0
41	37.69153005377049	40.0	35.0	41.0	34.0	41.0
42	37.596631157446964	40.0	35.0	41.0	34.0	41.0
43	37.610384713435586	40.0	35.0	41.0	34.0	41.0
44	37.567424850907145	40.0	35.0	41.0	34.0	41.0
45	37.51889060621673	40.0	35.0	41.0	34.0	41.0
46	37.46628064123528	39.0	35.0	41.0	34.0	41.0
47	37.367824422961846	39.0	35.0	41.0	34.0	41.0
48	37.32550242505891	39.0	35.0	41.0	34.0	41.0
49	37.26766421014119	39.0	35.0	41.0	34.0	41.0
50	37.15729709664929	39.0	35.0	41.0	34.0	41.0
51	37.062517666610134	39.0	35.0	41.0	34.0	41.0
52	36.759061571002974	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	3.0
14	9.0
15	18.0
16	29.0
17	58.0
18	121.0
19	257.0
20	461.0
21	812.0
22	1347.0
23	2199.0
24	3707.0
25	6009.0
26	8495.0
27	9949.0
28	10707.0
29	11583.0
30	13557.0
31	16628.0
32	20957.0
33	29091.0
34	83565.0
35	284150.0
36	76533.0
37	115807.0
38	211322.0
39	960393.0
40	7232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.62501559995008	22.940033258560238	25.41229201399889	19.022659127490794
2	26.382048910776817	23.94381671311985	26.787807612348974	22.886326763754354
3	26.219649430455156	25.026693247914938	25.93906366166295	22.814593659966956
4	25.442211918255197	27.66215148111526	24.119762816758985	22.77587378387056
5	24.739334167463998	34.91844826096556	21.272891926745835	19.069325644824602
6	87.4959866795093	2.8072976833140797	7.17907036030818	2.517645276868447
7	88.71048945976706	2.569218445167642	5.2430232223256885	3.477268872739607
8	78.64550833437333	4.88345103962334	10.016661280017237	6.4543793459860925
9	47.36672842646904	20.698600431145287	15.507150377118792	16.427520765266884
10	36.923668510927435	24.355122063609397	24.29554892091012	14.425660504553054
11	34.34090344244232	19.88436303670495	26.51815514190354	19.25657837894919
12	23.227979003800524	29.32609282316963	27.59292503597322	19.85300313705663
13	17.386024364722033	31.49888587023188	28.132229976864075	22.98285978818201
14	16.475680611155376	25.728024336988785	35.7715655309903	22.02472952086553
15	18.731620058815814	20.371668143995272	38.73998269872203	22.156729098466883
16	19.357804721691558	23.583444532977495	27.151273115926028	29.907477629404923
17	21.53390442483917	23.705630808648078	29.681345019695936	25.079119746816808
18	24.852240472830488	27.17591303707828	26.079863211104392	21.89198327898684
19	27.256446112705774	22.951393222208356	25.781197500168002	24.01096316491787
20	22.443768179941827	28.503588788515877	26.74716774239656	22.30547528914574
21	22.112889238754434	24.79629398519258	26.623968136635295	26.46684863941769
22	20.833320000042665	28.264869552417434	24.139122754807186	26.76268769273272
23	20.597534087890917	23.435071674437307	33.841705039877205	22.125689197794568
24	21.158118960685993	23.415818402714443	31.09456716405174	24.33149547254782
25	19.22804513692223	29.820491241094693	27.482632055577422	23.468831566405655
26	19.577804017693808	28.2421496251212	27.27004606918591	24.91000028799908
27	21.677370632413975	26.443328714681446	31.65787202814284	20.221428624761735
28	19.21156518965806	24.870853746601345	29.910517619676952	26.007063444063643
29	22.654060840338644	26.10119647617128	28.017457010804232	23.227285672685845
30	24.842480504062387	27.35271247132009	23.997896540064406	23.806910484553114
31	27.055166756799707	25.426318635780365	24.354962064121395	23.163552543298525
32	23.63517770076469	26.990313630996383	23.967603303669428	25.4069053645695
33	19.907616295627854	27.028286842815437	27.44241885092634	25.621678010630365
34	21.386224897413662	25.0846663957342	26.75634104637532	26.77276766047682
35	24.909893621673742	24.511761562363	25.031599898880323	25.546744917082933
36	21.22510541299601	28.366415894135805	27.02364685766339	23.384831835204796
37	20.691347121022545	26.47676860767379	27.72529794571324	25.106586325590424
38	19.719723563551263	27.215966242241358	25.555384889435018	27.508925304772358
39	24.233682452216154	24.47181502352526	26.55650168586127	24.738000838397316
40	18.155781901497914	23.97997659740822	29.87670439454594	27.987537106547926
41	21.302118499887467	26.85122074276029	23.987123241205627	27.859537516146617
42	21.83993011222364	25.098746350678347	26.719327831484275	26.34199570561374
43	23.166805866221228	26.360288980408598	26.469355298063046	24.00354985530713
44	20.13902888844089	28.183696478837934	24.94077352285806	26.736501109863113
45	21.512304493958954	26.29148920056789	26.315702456418805	25.88050384905435
46	23.070859506582913	24.722374221735823	26.730901127783056	25.475865143898208
47	20.91081308539813	22.968459834261864	30.39792939329261	25.7227976870474
48	20.164522140195817	23.005686381803578	27.908924024776454	28.920867453224147
49	20.199348695417505	24.63714782779362	29.72619820949906	25.437305267289812
50	19.295884919834926	25.311012338093853	28.252922923979977	27.140179818091248
51	20.321214972112088	22.812247000809595	28.30433609279117	28.562201934287145
52	21.66083735198714	21.690330590942107	30.66886185964205	25.979970197428703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	269.0
1	592.5
2	916.0
3	3865.5
4	6815.0
5	4685.5
6	2556.0
7	2713.0
8	2870.0
9	2912.5
10	2955.0
11	2868.0
12	2781.0
13	2823.5
14	2732.5
15	2599.0
16	2559.0
17	2519.0
18	2613.0
19	2707.0
20	2582.5
21	2458.0
22	2927.0
23	3396.0
24	4451.0
25	5506.0
26	6966.5
27	8427.0
28	10201.5
29	11976.0
30	14463.0
31	16950.0
32	18357.5
33	19765.0
34	25587.5
35	31410.0
36	37218.5
37	43027.0
38	45205.0
39	53084.0
40	58785.0
41	71976.5
42	85168.0
43	105573.5
44	125979.0
45	136546.5
46	147114.0
47	153905.0
48	160696.0
49	179643.0
50	198590.0
51	207553.5
52	216517.0
53	194993.0
54	173469.0
55	148611.5
56	123754.0
57	105169.5
58	86585.0
59	78442.5
60	70300.0
61	64834.5
62	59369.0
63	55480.5
64	42837.0
65	34082.0
66	27932.5
67	21783.0
68	18195.5
69	14608.0
70	12079.5
71	9551.0
72	7614.0
73	5677.0
74	5048.5
75	4420.0
76	4222.0
77	4024.0
78	2752.0
79	1480.0
80	1125.0
81	770.0
82	572.5
83	375.0
84	253.0
85	131.0
86	74.0
87	17.0
88	13.0
89	7.0
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1875006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.119926130905057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.3647410412052	16.73072430003228
2	12.894239732442978	6.220162197214112
3	5.082786746801005	3.677893226159503
4	2.513319336851644	2.4248430699294774
5	1.413400963743911	1.7045563419426577
6	0.8748734212604495	1.2661129376816536
7	0.6112533638833282	1.0320370188893173
8	0.4417702343800897	0.8524372336064302
9	0.34184131798934964	0.7420668604554904
>10	4.631477451661192	28.70787330647001
>50	1.637853094060751	26.07439982528421
>100	0.18201096319430066	6.974997280632585
>500	0.006658935654700047	1.173492379438561
>1k	0.003107503305526688	1.3727060818835124
>5k	6.658935654700047E-4	1.0456979403802171
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	7693	0.41029202039886803	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCTT	6718	0.3582921867983356	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	5121	0.27311912601879673	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3669	0.19567937382600373	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	2423	0.12922625314265662	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2389	0.12741292561197137	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	2218	0.11829295479587798	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	2154	0.11487963238517637	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1893	0.10095967692903382	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36533216427040766	0.0
2	0.0	0.0	0.0	1.256155980300863	0.0
3	0.0	0.0	0.0	2.0002069326711487	0.0
4	0.0	0.0	0.0	2.4567388051024905	0.0
5	0.0	0.0	0.0	4.814171261318631	0.0
6	0.0	0.0	0.0	5.169210125194266	0.0
7	0.0	0.0	0.0	6.39549953440149	0.0
8	0.0	0.0	0.0	7.987494440017792	0.0
9	0.0	0.0	0.0	9.157144030472436	0.0
10	0.0	0.0	0.0	10.114527633511573	0.0
11	0.0	0.0	0.0	10.98369818549914	0.0
12	0.0	0.0	0.0	11.712389187021268	0.0
13	0.0	0.0	0.0	12.053988093904767	0.0
14	0.0	0.0	0.0	12.306733951784688	0.0
15	0.0	0.0	0.0	13.173877843590901	0.0
16	5.33331626672128E-5	0.0	0.0	14.155261369830283	0.0
17	5.33331626672128E-5	0.0	0.0	15.192911382683576	0.0
18	5.33331626672128E-5	0.0	0.0	15.827576018423407	0.0
19	1.066663253344256E-4	0.0	0.0	16.490507230376863	0.0
20	1.066663253344256E-4	0.0	0.0	17.389757686108737	0.0
21	1.066663253344256E-4	0.0	0.0	18.220101695674575	0.0
22	1.066663253344256E-4	0.0	0.0	19.083032267630077	0.0
23	1.066663253344256E-4	0.0	0.0	19.74031016434081	0.0
24	1.066663253344256E-4	0.0	0.0	20.343721566757655	0.0
25	1.599994880016384E-4	0.0	0.0	20.932573015766348	0.0
26	1.599994880016384E-4	0.0	0.0	21.481957924401307	0.0
27	1.599994880016384E-4	0.0	0.0	22.02174286375617	0.0
28	1.599994880016384E-4	0.0	0.0	22.589421047185983	0.0
29	1.599994880016384E-4	0.0	0.0	23.259498902936844	0.0
30	1.599994880016384E-4	0.0	0.0	23.871603610868444	0.0
31	1.599994880016384E-4	0.0	0.0	24.447015102885004	0.0
32	1.599994880016384E-4	0.0	0.0	24.999760000768	0.0
33	1.599994880016384E-4	0.0	0.0	25.53239829632545	0.0
34	2.133326506688512E-4	0.0	0.0	26.216822772833794	0.0
35	2.133326506688512E-4	0.0	0.0	26.734367783356426	0.0
36	2.66665813336064E-4	0.0	0.0	27.285672685847405	0.0
37	2.66665813336064E-4	0.0	0.0	27.81447099369282	0.0
38	2.66665813336064E-4	0.0	0.0	28.31750938396997	0.0
39	2.66665813336064E-4	0.0	0.0	28.881720911826417	0.0
40	2.66665813336064E-4	0.0	0.0	29.42187918331995	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTA	20	6.31248E-4	46.0	31
CTAGCGG	455	0.0	43.472527	1
CATGCGG	250	0.0	42.32	1
CGTTTTT	2820	0.0	41.921986	1
TGGGCGA	655	0.0	41.786263	5
TACGGGA	435	0.0	41.77012	3
ATACCGG	100	0.0	41.399998	1
CGGGTAT	370	0.0	41.027027	5
ATAGCGG	350	0.0	40.74286	1
CTTGCGG	645	0.0	40.651165	1
AATGCGG	340	0.0	40.588234	1
ATGGGCG	340	0.0	39.911766	4
GGGCGAT	3065	0.0	39.771614	6
AGGGCGA	1415	0.0	39.660778	5
GGACTAA	1290	0.0	39.581398	7
CACATCG	35	5.37493E-6	39.42857	35
CTTACGG	280	0.0	39.42857	1
GTCGAGG	235	0.0	39.148937	1
AATACGG	100	0.0	39.1	1
CGTAGGG	830	0.0	39.072292	2
>>END_MODULE