FastQCFastQC Report
Thu 26 May 2016
SRR1544873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544873_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1425443
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT99570.6985196882653323No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCTT81600.5724536161740595Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT61310.4301119020543087No Hit
TGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCTTG39140.2745813056011359Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA37400.2623745740797773No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC25080.1759453026182036No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT24330.17068378041072144No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC24150.16942101508092572No Hit
GCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC21810.15300506579358136No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17630.12368084869054743No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.11869994100079766No Hit
AGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC16760.11757748292986812No Hit
ACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT16010.11231596072238596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG1450.046.0000041
AGTACGG750.046.0000041
TCGTATA206.3119683E-446.013
CATGCGG2350.044.0425531
CTAGCGG3050.043.7377051
CTTGCGG3750.043.546671
CGTTCTG1350.042.5925941
CGTTTTT17800.042.1235961
GTCGAGG1650.041.818181
AATGCGG2650.041.6603781
TACGGGA3050.041.4754073
ACTAATC8250.040.4242449
CGTTGAT1650.040.4242424
GGACTAA9350.040.3422437
CTAATCC8350.039.9401210
TGGGCGA5200.039.807695
GCGGGTC1850.039.7837834
CTTACGG1100.039.7272721
ATGGGCG1800.039.611114
GGTAATC1400.039.4285747
Produced by FastQC (version 0.11.5)