##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544873_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1425443
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.861446581869636	34.0	33.0	34.0	31.0	34.0
2	33.22741561746068	34.0	33.0	34.0	31.0	34.0
3	33.32834494258978	34.0	34.0	34.0	31.0	34.0
4	36.58532680717503	37.0	37.0	37.0	35.0	37.0
5	36.58265325235734	37.0	37.0	37.0	35.0	37.0
6	36.52150945355233	37.0	37.0	37.0	35.0	37.0
7	36.404894478418285	37.0	37.0	37.0	35.0	37.0
8	36.43588063500259	37.0	37.0	37.0	35.0	37.0
9	38.26084662803073	39.0	39.0	39.0	37.0	39.0
10	38.16677902939647	39.0	39.0	39.0	35.0	39.0
11	37.75735262651681	39.0	37.0	39.0	35.0	39.0
12	37.76097325533185	39.0	37.0	39.0	35.0	39.0
13	37.747403438790606	39.0	37.0	39.0	35.0	39.0
14	39.08827571498825	41.0	38.0	41.0	35.0	41.0
15	39.13799920445784	41.0	38.0	41.0	35.0	41.0
16	39.144383184736256	41.0	38.0	41.0	35.0	41.0
17	39.192329682772304	41.0	38.0	41.0	35.0	41.0
18	39.17901733005108	41.0	38.0	41.0	35.0	41.0
19	39.13085335576379	41.0	38.0	41.0	35.0	41.0
20	39.069429643977344	41.0	38.0	41.0	35.0	41.0
21	39.01927050046898	41.0	38.0	41.0	35.0	41.0
22	38.9837769731936	41.0	38.0	41.0	35.0	41.0
23	38.95536896249096	41.0	38.0	41.0	35.0	41.0
24	38.87538610803799	41.0	37.0	41.0	35.0	41.0
25	38.82488251020911	41.0	37.0	41.0	35.0	41.0
26	38.77337852162451	41.0	37.0	41.0	35.0	41.0
27	38.743584976740564	41.0	37.0	41.0	35.0	41.0
28	38.78909994998046	41.0	37.0	41.0	35.0	41.0
29	38.83212026015772	41.0	37.0	41.0	35.0	41.0
30	38.75400980607432	41.0	37.0	41.0	35.0	41.0
31	38.77401762118864	41.0	37.0	41.0	35.0	41.0
32	38.720977268119455	41.0	37.0	41.0	35.0	41.0
33	38.653603827020795	41.0	36.0	41.0	35.0	41.0
34	38.59566745215347	41.0	36.0	41.0	35.0	41.0
35	38.53173785272368	41.0	36.0	41.0	35.0	41.0
36	38.4988435174188	40.0	36.0	41.0	35.0	41.0
37	38.443292365952196	40.0	36.0	41.0	35.0	41.0
38	38.404312904830284	40.0	36.0	41.0	35.0	41.0
39	38.374416935647375	40.0	36.0	41.0	35.0	41.0
40	38.294984787185456	40.0	36.0	41.0	35.0	41.0
41	38.25597866768436	40.0	35.0	41.0	35.0	41.0
42	38.19197891462514	40.0	35.0	41.0	35.0	41.0
43	38.11477063621625	40.0	35.0	41.0	35.0	41.0
44	37.97091781291851	40.0	35.0	41.0	35.0	41.0
45	37.925132046669	40.0	35.0	41.0	35.0	41.0
46	37.87169322098463	40.0	35.0	41.0	35.0	41.0
47	37.78122590661289	40.0	35.0	41.0	35.0	41.0
48	37.71056226029382	40.0	35.0	41.0	35.0	41.0
49	37.65867523289251	39.0	35.0	41.0	35.0	41.0
50	37.55258189910084	39.0	35.0	41.0	35.0	41.0
51	37.453145443206076	39.0	35.0	41.0	35.0	41.0
52	36.978789050140904	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	6.0
14	6.0
15	13.0
16	39.0
17	36.0
18	104.0
19	196.0
20	322.0
21	517.0
22	846.0
23	1294.0
24	2153.0
25	3532.0
26	4878.0
27	5638.0
28	5905.0
29	6665.0
30	7722.0
31	9681.0
32	13058.0
33	18461.0
34	55005.0
35	200426.0
36	53183.0
37	83716.0
38	160565.0
39	785462.0
40	6010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.75346681698251	22.440181754023136	24.29588555978738	20.510465869206975
2	26.41347286422537	24.051821082989637	26.587524018848878	22.947182033936116
3	26.108094115303103	25.01320642074078	25.749117993493954	23.129581470462167
4	25.863538563099333	26.913036859418437	24.285923744407878	22.93750083307435
5	24.52234147559741	33.9366779310011	21.348731587303035	20.192249006098457
6	84.90343002140386	2.89902858269324	8.601606658421277	3.595934737481611
7	86.5079136801682	2.6322343299591777	6.216172796807729	4.6436791930648935
8	74.86514718582224	4.988905203505157	12.151099693218178	7.994847917454434
9	45.607926798896905	19.778693360590356	16.62458618127838	17.988793659234357
10	34.97930117163576	23.476561321638258	26.602256281029828	14.941881225696152
11	32.14130624654932	19.52859567166137	27.299513203965365	21.030584877823948
12	22.39163544245543	27.572480976089537	29.357890845161823	20.67799273629321
13	16.863248828609773	29.98366121970503	29.571087725008997	23.5820022266762
14	15.957495318999076	25.75423920844257	34.778240869680516	23.51002460287784
15	17.89976870348376	20.217925234470968	38.73546679874257	23.146839263302706
16	18.341526108023963	24.584918513051733	27.407128871515734	29.666426507408573
17	20.37436782810677	23.847463560451033	29.754258851458808	26.02390975998339
18	23.461127523162975	27.579426185403417	27.02738727539439	21.932059016039222
19	25.614773793129576	22.773271186571474	26.048323223026102	25.563631797272844
20	21.07730719502639	29.053774861569348	27.176183123421982	22.69273481998228
21	21.23529316850972	24.53749465955496	27.20719102763141	27.020021144303914
22	19.935977797779355	28.000067347484254	24.216892573045712	27.847062281690675
23	19.58121089373619	23.245685727173935	34.136896389403155	23.036206989686715
24	21.70595386837636	23.12382887284865	30.224358322289984	24.945858936485006
25	18.954879290157518	29.422432184240265	27.450904736281984	24.171783789320234
26	19.752596210441244	27.04176876942817	26.858317028460625	26.34731799166996
27	22.822939956210103	25.77837205696755	30.547977014864852	20.85071097195749
28	19.54606392539021	24.6242746991637	28.77743971523239	27.052221660213704
29	23.74181219452479	24.984864354449808	26.834394640823938	24.43892881020146
30	24.06409796814043	27.497837514372726	23.501886781863604	24.93617773562324
31	27.465005615798038	24.3686348735095	23.539980202645776	24.62637930804669
32	22.87948378153318	26.219287617954556	23.27157241643475	27.62965618407751
33	19.68307396367305	26.300595674467512	27.667118222194787	26.34921213966465
34	21.700411731651144	23.914600583818505	26.24924321772249	28.13574446680786
35	24.225030394059953	23.483085609175532	24.924742694025646	27.36714130273887
36	21.234591632215388	28.23108324920744	26.0396241729764	24.494700945600773
37	20.399132059296655	25.418975013381807	28.262722536081764	25.919170391239778
38	19.203152984721243	26.40736949846469	24.830386062438134	29.559091454375935
39	24.649740466647913	23.44807894808842	26.423294372346	25.47888621291767
40	18.13730889274422	23.062023525318093	30.078017851292543	28.722649730645138
41	21.26812506708441	26.699348904165234	23.310858448917283	28.721667579833078
42	21.53491931981847	23.596804642486582	27.318314376653436	27.549961661041518
43	24.154385689220824	24.86840932959087	26.300806135355813	24.67639884583249
44	20.196247762976142	26.152150594587088	25.659952730484488	27.991648911952282
45	21.17117275120787	24.441033419084455	26.652135511556757	27.735658318150918
46	23.699509555976633	22.978260091774978	26.604080275395088	26.718150076853302
47	19.888062868876553	21.72208920314597	31.75602251370276	26.63382541427472
48	19.977929668180348	21.373706279381217	27.704299645794322	30.944064406644113
49	20.26057864116629	22.478275174805308	30.653067151755632	26.608079032272773
50	19.471911539079432	22.87927332064488	29.433446304061263	28.215368836214427
51	20.00290436025853	21.06531092439333	28.449541651262102	30.48224306408604
52	21.007013258334425	20.383277339044774	32.614492477075544	25.99521692554525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	161.0
1	351.0
2	541.0
3	1991.0
4	3441.0
5	2482.0
6	1523.0
7	1589.0
8	1655.0
9	1664.0
10	1673.0
11	1670.0
12	1667.0
13	1675.0
14	1669.5
15	1656.0
16	1597.5
17	1539.0
18	1600.5
19	1662.0
20	1603.0
21	1544.0
22	1911.0
23	2278.0
24	2853.0
25	3428.0
26	4417.0
27	5406.0
28	6412.0
29	7418.0
30	9063.5
31	10709.0
32	11701.0
33	12693.0
34	16384.5
35	20076.0
36	24240.5
37	28405.0
38	30021.5
39	35588.0
40	39538.0
41	48745.0
42	57952.0
43	72027.5
44	86103.0
45	94760.5
46	103418.0
47	112750.5
48	122083.0
49	146961.0
50	171839.0
51	180455.0
52	189071.0
53	167892.5
54	146714.0
55	123327.5
56	99941.0
57	83408.0
58	66875.0
59	58836.5
60	50798.0
61	46632.0
62	42466.0
63	40698.5
64	31419.0
65	23907.0
66	19855.0
67	15803.0
68	13093.5
69	10384.0
70	8589.5
71	6795.0
72	5419.5
73	4044.0
74	3612.0
75	3180.0
76	3012.0
77	2844.0
78	1936.5
79	1029.0
80	792.0
81	555.0
82	418.5
83	282.0
84	182.0
85	82.0
86	45.0
87	8.0
88	5.0
89	1.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1425443.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.586146071561913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.75326561002666	19.242237740866518
2	12.722617303576527	7.019359586980865
3	4.922678559360638	4.073931894056057
4	2.3394582365708683	2.581465465694505
5	1.3111461534967446	1.8084734655763866
6	0.8805179680962887	1.4574058371923466
7	0.6078257153827574	1.1737298279420252
8	0.4706564527496855	1.03868781240608
9	0.3815106599713686	0.9471967914445347
>10	6.051483086383996	42.1100591332622
>50	0.436818697614495	7.708948146131908
>100	0.10925779569462622	5.304924325493947
>500	0.007402981423688483	1.4980573278435847
>1k	0.004594953987116989	2.327964045684822
>5k	7.658256645194983E-4	1.7075585994242108
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	9957	0.6985196882653323	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCTT	8160	0.5724536161740595	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	6131	0.4301119020543087	No Hit
TGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCTTG	3914	0.2745813056011359	Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3740	0.2623745740797773	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	2508	0.1759453026182036	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	2433	0.17068378041072144	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	2415	0.16942101508092572	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	2181	0.15300506579358136	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1763	0.12368084869054743	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1692	0.11869994100079766	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1676	0.11757748292986812	No Hit
ACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	1601	0.11231596072238596	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.582275124294693	0.0
2	0.0	0.0	0.0	2.0168466925720634	0.0
3	0.0	0.0	0.0	3.133482012258645	0.0
4	0.0	0.0	0.0	3.8160066730132316	0.0
5	0.0	0.0	0.0	7.256551121300536	0.0
6	0.0	0.0	0.0	7.763411093954652	0.0
7	0.0	0.0	0.0	9.486173771943179	0.0
8	0.0	0.0	0.0	11.638416969321117	0.0
9	0.0	0.0	0.0	13.227536983239597	0.0
10	0.0	0.0	0.0	14.568523609853218	0.0
11	7.015362943309553E-5	0.0	0.0	15.75517225171403	0.0
12	7.015362943309553E-5	0.0	0.0	16.746232574715368	0.0
13	7.015362943309553E-5	0.0	0.0	17.18883182280877	0.0
14	7.015362943309553E-5	0.0	0.0	17.525920012234792	0.0
15	7.015362943309553E-5	0.0	0.0	18.644870401692668	0.0
16	7.015362943309553E-5	0.0	0.0	19.86673616552889	0.0
17	7.015362943309553E-5	0.0	0.0	21.12858949814198	0.0
18	7.015362943309553E-5	0.0	0.0	21.908697857438003	0.0
19	7.015362943309553E-5	0.0	0.0	22.70725662127493	0.0
20	7.015362943309553E-5	0.0	0.0	23.780957919748456	0.0
21	7.015362943309553E-5	0.0	0.0	24.785136971453788	0.0
22	7.015362943309553E-5	0.0	0.0	25.798856916762016	0.0
23	7.015362943309553E-5	0.0	0.0	26.59643352978688	0.0
24	1.4030725886619107E-4	0.0	0.0	27.291726151098292	0.0
25	1.4030725886619107E-4	0.0	0.0	27.94829396896263	0.0
26	1.4030725886619107E-4	0.0	0.0	28.59321628434108	0.0
27	1.4030725886619107E-4	0.0	0.0	29.210077147946286	0.0
28	2.104608882992866E-4	0.0	0.0	29.83633859789553	0.0
29	2.104608882992866E-4	0.0	0.0	30.57561754486149	0.0
30	2.104608882992866E-4	0.0	0.0	31.232395823614134	0.0
31	2.104608882992866E-4	0.0	0.0	31.856342203792085	0.0
32	2.104608882992866E-4	0.0	0.0	32.461978486688	0.0
33	2.104608882992866E-4	0.0	0.0	33.048462828748676	0.0
34	2.104608882992866E-4	0.0	0.0	33.74473760087215	0.0
35	2.104608882992866E-4	0.0	0.0	34.289129765272975	0.0
36	2.8061451773238213E-4	0.0	0.0	34.87175565771483	0.0
37	2.8061451773238213E-4	0.0	0.0	35.42540810120082	0.0
38	2.8061451773238213E-4	0.0	0.0	35.957383073191984	0.0
39	3.5076814716547767E-4	0.0	0.0	36.54344649347606	0.0
40	3.5076814716547767E-4	0.0	0.0	37.08706696795312	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGG	145	0.0	46.000004	1
AGTACGG	75	0.0	46.000004	1
TCGTATA	20	6.3119683E-4	46.0	13
CATGCGG	235	0.0	44.042553	1
CTAGCGG	305	0.0	43.737705	1
CTTGCGG	375	0.0	43.54667	1
CGTTCTG	135	0.0	42.592594	1
CGTTTTT	1780	0.0	42.123596	1
GTCGAGG	165	0.0	41.81818	1
AATGCGG	265	0.0	41.660378	1
TACGGGA	305	0.0	41.475407	3
ACTAATC	825	0.0	40.424244	9
CGTTGAT	165	0.0	40.42424	24
GGACTAA	935	0.0	40.342243	7
CTAATCC	835	0.0	39.94012	10
TGGGCGA	520	0.0	39.80769	5
GCGGGTC	185	0.0	39.783783	4
CTTACGG	110	0.0	39.727272	1
ATGGGCG	180	0.0	39.61111	4
GGTAATC	140	0.0	39.428574	7
>>END_MODULE