FastQCFastQC Report
Thu 26 May 2016
SRR1544872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544872_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1178178
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT48030.40766335816829036No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT39690.3368760917280751Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT36920.3133652130662769No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21610.18341880428933488No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.17323358609649817No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT14360.12188311104094626No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC13740.11662074830798062No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC13510.11466858148768692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG700.046.0000041
CGGTCTA351.0195981E-746.00000430
ACGGTCT351.0195981E-746.00000429
CTATCGC206.311519E-446.019
CGACCGT206.311519E-446.022
CCGTATT206.311519E-446.023
GTATCGC253.4172088E-546.05
GCGATAT301.8614428E-646.08
CGTGTAT206.311519E-446.021
TAGGCGT301.8614428E-646.044
AACTCGA301.8614428E-646.018
CAATCTA206.311519E-446.025
TAGTCGA206.311519E-446.042
GCTACGA1600.044.562510
CTAGCGG3700.044.1351361
CTAACGG1100.043.9090921
GGCGATA2200.043.9090927
GTTACGG1050.043.8095251
ATAGCGG2050.043.7560961
TACGGGT900.043.4444433
Produced by FastQC (version 0.11.5)