##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544872_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1178178
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90621281334399	34.0	33.0	34.0	31.0	34.0
2	33.22585212081706	34.0	33.0	34.0	31.0	34.0
3	33.2811018369041	34.0	34.0	34.0	31.0	34.0
4	36.56613431926245	37.0	37.0	37.0	35.0	37.0
5	36.56255506383585	37.0	37.0	37.0	35.0	37.0
6	36.67031976492516	37.0	37.0	37.0	35.0	37.0
7	36.645384653252734	37.0	37.0	37.0	35.0	37.0
8	36.53724819169938	37.0	37.0	37.0	35.0	37.0
9	38.33434591377534	39.0	39.0	39.0	37.0	39.0
10	38.129811454635885	39.0	39.0	39.0	35.0	39.0
11	37.430923001448	39.0	35.0	39.0	35.0	39.0
12	37.34133806606472	39.0	35.0	39.0	35.0	39.0
13	37.33280030691458	39.0	35.0	39.0	35.0	39.0
14	38.62426051072079	41.0	35.0	41.0	35.0	41.0
15	38.689916973496366	41.0	35.0	41.0	35.0	41.0
16	38.652108594796374	41.0	35.0	41.0	35.0	41.0
17	38.73606449959174	41.0	36.0	41.0	35.0	41.0
18	38.72243413134518	41.0	36.0	41.0	35.0	41.0
19	38.64544915963462	41.0	35.0	41.0	35.0	41.0
20	38.56732429225465	41.0	35.0	41.0	35.0	41.0
21	38.5046020210868	40.0	35.0	41.0	35.0	41.0
22	38.48664038880373	40.0	35.0	41.0	35.0	41.0
23	38.43650195471312	40.0	35.0	41.0	35.0	41.0
24	38.380762499384645	40.0	35.0	41.0	35.0	41.0
25	38.342699490229826	40.0	35.0	41.0	35.0	41.0
26	38.28112475364504	40.0	35.0	41.0	35.0	41.0
27	38.25953718368532	40.0	35.0	41.0	35.0	41.0
28	38.300868799111846	40.0	35.0	41.0	35.0	41.0
29	38.26701058753431	40.0	36.0	41.0	35.0	41.0
30	38.20030929112579	40.0	35.0	41.0	35.0	41.0
31	38.14828065029223	40.0	35.0	41.0	35.0	41.0
32	38.07059459606273	40.0	35.0	41.0	35.0	41.0
33	37.95616282089803	40.0	35.0	41.0	35.0	41.0
34	37.90759291040912	40.0	35.0	41.0	35.0	41.0
35	37.83683789715985	40.0	35.0	41.0	35.0	41.0
36	37.77300119336807	40.0	35.0	41.0	35.0	41.0
37	37.70016415176654	40.0	35.0	41.0	35.0	41.0
38	37.65272310296067	40.0	35.0	41.0	35.0	41.0
39	37.5326555070626	40.0	35.0	41.0	34.0	41.0
40	37.44442435693079	39.0	35.0	41.0	34.0	41.0
41	37.39466277591332	39.0	35.0	41.0	34.0	41.0
42	37.31706923741574	39.0	35.0	41.0	34.0	41.0
43	37.28514961236757	39.0	35.0	41.0	34.0	41.0
44	37.22971571358487	39.0	35.0	41.0	34.0	41.0
45	37.17997620054015	39.0	35.0	41.0	34.0	41.0
46	37.118359025546226	39.0	35.0	41.0	34.0	41.0
47	37.000013580290926	38.0	35.0	41.0	34.0	41.0
48	36.904029781578	38.0	35.0	41.0	34.0	41.0
49	36.77301052981807	37.0	35.0	40.0	34.0	41.0
50	36.64431011273339	37.0	35.0	40.0	34.0	41.0
51	36.52264258881086	37.0	35.0	40.0	34.0	41.0
52	36.24643814432115	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	4.0
14	4.0
15	13.0
16	17.0
17	50.0
18	98.0
19	180.0
20	289.0
21	512.0
22	829.0
23	1398.0
24	2270.0
25	3751.0
26	5446.0
27	6258.0
28	6675.0
29	7280.0
30	8567.0
31	10664.0
32	13528.0
33	19461.0
34	60855.0
35	237083.0
36	44397.0
37	70094.0
38	129109.0
39	544976.0
40	4367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.92950810488738	21.860958191376856	24.42780292960826	19.78173077412751
2	27.637165182171113	23.82025466440555	26.274128357514737	22.268451795908597
3	28.4395906221301	24.550619685650215	25.08059053895082	21.92919915326886
4	26.518658470961093	26.773883063509928	23.016471195354182	23.690987270174794
5	25.21893975273685	35.46857945064328	20.389703423421587	18.922777373198276
6	87.44247473641505	2.7737744211825377	7.123796234524835	2.6599546078775873
7	88.3145840441767	2.7382110343258828	5.143535187382551	3.8036697341148793
8	78.48406607490548	5.235456781572903	10.098643838197624	6.181833305323983
9	51.228507067692654	22.180604288995383	13.293831662108783	13.297056981203179
10	42.44613292728264	21.3762266822161	23.11738973228154	13.060250658219728
11	40.76557192546457	17.914780279380533	23.344435221163526	17.975212573991367
12	21.545216427398916	33.380779474748294	26.1766897701366	18.897314327716185
13	15.212472139184403	36.195549399157	28.81381251389858	19.778165947760016
14	15.116221827262095	25.588323665863733	41.06671487669944	18.22873963017473
15	17.108790013054055	18.424041189022372	44.93217493451753	19.53499386340604
16	16.507098248312225	20.815869927973534	28.446380767591993	34.230651056122255
17	19.98297371025431	24.371444722274564	30.378092274681755	25.267489292789374
18	24.77019601452412	27.768554496858712	25.781588181072806	21.67966130754436
19	29.639069817973173	22.474362957040448	24.593652232514952	23.292914992471424
20	22.552874013943562	28.964129359061197	26.699276340247398	21.783720286747844
21	21.435470701371102	23.828317962141544	26.201983061982144	28.534228274505207
22	20.685159627832128	29.036274654593786	23.076479105873645	27.202086611700437
23	20.721656659689792	21.953219292840302	35.798410766454644	21.526713281015265
24	20.258059478279172	21.6991829757473	32.56859320068784	25.474164345285686
25	18.661950910643384	30.955763899852144	27.386099553717692	22.996185635786784
26	19.764331026381413	29.326044112179993	26.43250850041335	24.477116361025246
27	21.893381135957384	27.07078217383112	32.70032202264853	18.335514667562965
28	19.45690719059429	24.907187199217777	30.90831775843718	24.727587851750755
29	22.812936585133993	26.912826415023876	27.50034375111401	22.773893248728122
30	29.17903746293005	25.619897842261523	22.491932458423094	22.709132236385337
31	30.246278575902792	26.31894331756322	23.322197494775832	20.112580611758155
32	27.19461745169236	28.578194466370952	23.54491426592586	20.682273816010824
33	21.386496777227208	27.72272101499094	26.53953816825641	24.351244039525437
34	22.395936776955605	24.64958605575728	29.73260407171073	23.221873095576388
35	28.043300757610478	26.972919202361613	23.79165117664733	21.192128863380578
36	23.149982430498618	28.606628200492622	28.001965747111218	20.24142362189754
37	22.318529118690044	29.41533452500386	26.840086981763367	21.42604937454273
38	21.240254019341727	28.176642239118365	26.53461531279654	24.048488428743365
39	25.57465849812168	24.051459117382944	27.87235884560737	22.501523538888012
40	17.652341157278443	22.98243559122645	32.29495033857363	27.070272912921478
41	22.862165139732706	26.140956629643398	24.00706854142583	26.98980968919807
42	24.24056466849661	23.73656612158774	26.796205666715895	25.226663543199756
43	23.469543651298867	28.24148812827943	26.355185718966062	21.93378250145564
44	20.628377036407063	30.346178591010865	24.555882048383182	24.46956232419889
45	21.82912938452424	28.670370691016128	25.90423518347822	23.59626474098141
46	23.794282358013817	24.565133621575008	27.987027427095057	23.653556593316118
47	23.022582326269887	21.675077959357584	32.24343010988153	23.058909604491003
48	21.165477542442655	21.648851022511028	29.78565208313175	27.40001935191457
49	20.042387483045857	26.499815817304345	30.16437244626873	23.29342425338107
50	19.319661375445815	27.46163992198123	28.096688276304597	25.122010426268353
51	21.14001449696056	23.007898636708546	29.256360244377333	26.59572662195356
52	23.370577281191807	21.21249929976625	31.59862092145669	23.818302497585258
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	201.0
1	403.0
2	605.0
3	2311.5
4	4018.0
5	2751.0
6	1484.0
7	1604.0
8	1724.0
9	1815.0
10	1906.0
11	1801.5
12	1697.0
13	1665.5
14	1526.5
15	1419.0
16	1461.5
17	1504.0
18	1427.5
19	1351.0
20	1678.5
21	2006.0
22	2367.0
23	2728.0
24	3025.0
25	3322.0
26	4312.5
27	5303.0
28	7636.0
29	9969.0
30	10446.5
31	10924.0
32	12262.0
33	13600.0
34	16837.0
35	20074.0
36	22233.0
37	24392.0
38	25376.5
39	30744.0
40	35127.0
41	50075.0
42	65023.0
43	88231.0
44	111439.0
45	125676.5
46	139914.0
47	136458.0
48	133002.0
49	118602.5
50	104203.0
51	92536.5
52	80870.0
53	73036.5
54	65203.0
55	59138.5
56	53074.0
57	49247.0
58	45420.0
59	46755.0
60	48090.0
61	48923.0
62	49756.0
63	46402.5
64	34176.5
65	25304.0
66	20511.5
67	15719.0
68	12307.5
69	8896.0
70	7623.5
71	6351.0
72	5500.0
73	4649.0
74	3811.5
75	2974.0
76	2658.5
77	2343.0
78	1517.5
79	692.0
80	665.0
81	638.0
82	410.5
83	183.0
84	100.0
85	17.0
86	12.0
87	7.0
88	4.0
89	5.5
90	10.0
91	5.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1178178.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.08694124161688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.7412824186527	15.182846656541738
2	12.574243463609486	5.554531530770556
3	5.117984089096409	3.3912184155420744
4	2.5897498034047897	2.2879860695316183
5	1.46394835539609	1.6167070653197553
6	0.9072986673277498	1.2023671412319188
7	0.611556688900051	0.9455191657557239
8	0.44829131279417656	0.7921107107849816
9	0.32602817542314516	0.648086863831431
>10	5.131062752064385	31.13683943598836
>50	1.8591063888492008	26.349590040147568
>100	0.21749345688702376	7.64803710303595
>500	0.00809816062877212	1.2816002660465236
>1k	0.0038562669660819615	1.9625595354717882
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	4803	0.40766335816829036	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT	3969	0.3368760917280751	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	3692	0.3133652130662769	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2161	0.18341880428933488	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2041	0.17323358609649817	No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	1436	0.12188311104094626	No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1374	0.11662074830798062	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1351	0.11466858148768692	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.487681827363947E-5	0.0	0.0	0.3443452517361553	0.0
2	8.487681827363947E-5	0.0	0.0	1.288939362303489	0.0
3	8.487681827363947E-5	0.0	0.0	2.033393935381581	0.0
4	8.487681827363947E-5	0.0	0.0	2.492153138150602	0.0
5	8.487681827363947E-5	0.0	0.0	4.895864631660071	0.0
6	8.487681827363947E-5	0.0	0.0	5.256081848413397	0.0
7	8.487681827363947E-5	0.0	0.0	6.604350106690161	0.0
8	8.487681827363947E-5	0.0	0.0	8.266153331669747	0.0
9	8.487681827363947E-5	0.0	0.0	9.402059790625865	0.0
10	8.487681827363947E-5	0.0	0.0	10.288004019766113	0.0
11	8.487681827363947E-5	0.0	0.0	11.14313796387303	0.0
12	8.487681827363947E-5	0.0	0.0	11.929606561996575	0.0
13	8.487681827363947E-5	0.0	0.0	12.275903980553023	0.0
14	8.487681827363947E-5	0.0	0.0	12.519330695361822	0.0
15	8.487681827363947E-5	0.0	0.0	13.350359623079026	0.0
16	8.487681827363947E-5	0.0	0.0	14.240377939496408	0.0
17	1.6975363654727894E-4	0.0	0.0	15.440196642612577	0.0
18	1.6975363654727894E-4	0.0	0.0	16.015576593689577	0.0
19	2.546304548209184E-4	0.0	0.0	16.627962837533886	0.0
20	2.546304548209184E-4	0.0	0.0	17.39550390518241	0.0
21	2.546304548209184E-4	0.0	0.0	18.093445981846546	0.0
22	2.546304548209184E-4	0.0	0.0	18.910300480912053	0.0
23	2.546304548209184E-4	0.0	0.0	19.496459787909806	0.0
24	3.395072730945579E-4	0.0	0.0	19.99799690708874	0.0
25	3.395072730945579E-4	0.0	0.0	20.48230403215813	0.0
26	3.395072730945579E-4	0.0	0.0	20.910762210803462	0.0
27	3.395072730945579E-4	0.0	0.0	21.369266783117663	0.0
28	3.395072730945579E-4	0.0	0.0	21.805448752225896	0.0
29	3.395072730945579E-4	0.0	0.0	22.38464816012521	0.0
30	3.395072730945579E-4	0.0	0.0	22.95552963983371	0.0
31	3.395072730945579E-4	0.0	0.0	23.484991232224672	0.0
32	3.395072730945579E-4	0.0	0.0	23.96182919728598	0.0
33	3.395072730945579E-4	0.0	0.0	24.4003028404876	0.0
34	3.395072730945579E-4	0.0	0.0	25.016169033881127	0.0
35	3.395072730945579E-4	0.0	0.0	25.511764775780907	0.0
36	3.395072730945579E-4	0.0	0.0	26.04606434681347	0.0
37	3.395072730945579E-4	0.0	0.0	26.520695514599662	0.0
38	3.395072730945579E-4	0.0	0.0	26.969184622357574	0.0
39	3.395072730945579E-4	0.0	0.0	27.539981225247796	0.0
40	3.395072730945579E-4	0.0	0.0	28.05823907762664	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGACGG	70	0.0	46.000004	1
CGGTCTA	35	1.0195981E-7	46.000004	30
ACGGTCT	35	1.0195981E-7	46.000004	29
CTATCGC	20	6.311519E-4	46.0	19
CGACCGT	20	6.311519E-4	46.0	22
CCGTATT	20	6.311519E-4	46.0	23
GTATCGC	25	3.4172088E-5	46.0	5
GCGATAT	30	1.8614428E-6	46.0	8
CGTGTAT	20	6.311519E-4	46.0	21
TAGGCGT	30	1.8614428E-6	46.0	44
AACTCGA	30	1.8614428E-6	46.0	18
CAATCTA	20	6.311519E-4	46.0	25
TAGTCGA	20	6.311519E-4	46.0	42
GCTACGA	160	0.0	44.5625	10
CTAGCGG	370	0.0	44.135136	1
CTAACGG	110	0.0	43.909092	1
GGCGATA	220	0.0	43.909092	7
GTTACGG	105	0.0	43.809525	1
ATAGCGG	205	0.0	43.756096	1
TACGGGT	90	0.0	43.444443	3
>>END_MODULE