FastQCFastQC Report
Thu 26 May 2016
SRR1544871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544871_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences789788
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT54600.691324760568659No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT40040.5069714910836832Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT33630.4258104706579487No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA31810.40276631197232676No Hit
TGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTTG21460.27171848647991614Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13800.1747304339898808No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC11950.15130642653471565No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT11820.14966041520002835No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC11780.14915395017397073No Hit
GCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC10530.13332691810966993No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC9390.1188926648670276No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.11357478209342255No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT8690.11002952691101917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT351.01903424E-746.00000427
TCGGGTA351.01903424E-746.0000044
GTTACGG351.01903424E-746.0000041
ATCTCGG453.092282E-1046.01
CGAACTA206.31025E-446.035
CGTATTT206.31025E-446.022
ATTACGG253.4161778E-546.01
ACGGGTA600.046.04
GCGATAT206.31025E-446.08
CGTGTTT253.4161778E-546.033
CCGAACT206.31025E-446.034
GCTACGA850.046.010
CGCCGTT206.31025E-446.045
ATGCCGG206.31025E-446.01
CCGTTGG206.31025E-446.01
ATAGCGG1300.044.2307661
CGTTGAT1100.043.90909224
ATCGAGG1100.043.9090921
GTCGGCG4800.043.6041683
ACATACG2750.043.49090616
Produced by FastQC (version 0.11.5)