##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544871_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	789788
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.90837161364822	34.0	33.0	34.0	31.0	34.0
2	33.25380481850826	34.0	33.0	34.0	31.0	34.0
3	33.34923675720573	34.0	34.0	34.0	31.0	34.0
4	36.57959984198291	37.0	37.0	37.0	35.0	37.0
5	36.58502155008686	37.0	37.0	37.0	35.0	37.0
6	36.521916007840076	37.0	37.0	37.0	35.0	37.0
7	36.407371345221755	37.0	37.0	37.0	35.0	37.0
8	36.43814162787989	37.0	37.0	37.0	35.0	37.0
9	38.285168931409444	39.0	39.0	39.0	37.0	39.0
10	38.149169144124755	39.0	39.0	39.0	35.0	39.0
11	37.536628816847056	39.0	35.0	39.0	35.0	39.0
12	37.491377432931365	39.0	35.0	39.0	35.0	39.0
13	37.48842220950432	39.0	35.0	39.0	35.0	39.0
14	38.76118781242561	41.0	35.0	41.0	35.0	41.0
15	38.82026949004037	41.0	35.0	41.0	35.0	41.0
16	38.7985383419348	41.0	35.0	41.0	35.0	41.0
17	38.88971470825082	41.0	36.0	41.0	35.0	41.0
18	38.88448545685678	41.0	36.0	41.0	35.0	41.0
19	38.82193069532583	41.0	36.0	41.0	35.0	41.0
20	38.75513555536423	41.0	35.0	41.0	35.0	41.0
21	38.70036389512122	41.0	35.0	41.0	35.0	41.0
22	38.68525730955649	41.0	35.0	41.0	35.0	41.0
23	38.64601639933754	41.0	35.0	41.0	35.0	41.0
24	38.57109882652053	41.0	35.0	41.0	35.0	41.0
25	38.52560307322978	40.0	35.0	41.0	35.0	41.0
26	38.46622258124965	40.0	35.0	41.0	35.0	41.0
27	38.448120255055784	40.0	35.0	41.0	35.0	41.0
28	38.49599259548132	40.0	36.0	41.0	35.0	41.0
29	38.499607489604806	40.0	36.0	41.0	35.0	41.0
30	38.44685662481577	40.0	35.0	41.0	35.0	41.0
31	38.42959123207747	40.0	35.0	41.0	35.0	41.0
32	38.37047283574833	40.0	35.0	41.0	35.0	41.0
33	38.291321215313474	40.0	35.0	41.0	35.0	41.0
34	38.25256524535698	40.0	35.0	41.0	35.0	41.0
35	38.16624841096598	40.0	35.0	41.0	35.0	41.0
36	38.136650594843175	40.0	35.0	41.0	35.0	41.0
37	38.0696034885311	40.0	35.0	41.0	35.0	41.0
38	38.00602946613522	40.0	35.0	41.0	35.0	41.0
39	37.955924881107336	40.0	35.0	41.0	35.0	41.0
40	37.88309267803512	40.0	35.0	41.0	35.0	41.0
41	37.838703550826295	40.0	35.0	41.0	35.0	41.0
42	37.77348478325829	40.0	35.0	41.0	35.0	41.0
43	37.688227726934315	40.0	35.0	41.0	35.0	41.0
44	37.539005403981825	39.0	35.0	41.0	35.0	41.0
45	37.49360967753372	39.0	35.0	41.0	35.0	41.0
46	37.422869175019116	39.0	35.0	41.0	35.0	41.0
47	37.30837769122853	39.0	35.0	41.0	35.0	41.0
48	37.147442098385895	38.0	35.0	41.0	35.0	41.0
49	37.00405931718385	38.0	35.0	40.0	35.0	41.0
50	36.86941559000643	37.0	35.0	40.0	34.0	41.0
51	36.734186895723916	37.0	35.0	40.0	34.0	41.0
52	36.29935754911445	37.0	35.0	39.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	7.0
14	11.0
15	18.0
16	22.0
17	26.0
18	66.0
19	114.0
20	204.0
21	326.0
22	413.0
23	758.0
24	1193.0
25	2028.0
26	2896.0
27	3431.0
28	3496.0
29	3749.0
30	4602.0
31	5564.0
32	7211.0
33	11084.0
34	35315.0
35	153045.0
36	28395.0
37	46122.0
38	91749.0
39	385968.0
40	1971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.86388245959675	21.506404250254498	23.344745678587163	22.284967611561584
2	26.977745926755027	24.228527149057722	26.354160863421576	22.439566060765674
3	28.48878433199795	24.42149032398568	25.040643818341124	22.049081525675245
4	26.52243386832922	26.217921771412072	23.397418041297158	23.862226318961543
5	24.976196143775294	34.51723753716187	20.62464863988817	19.881917679174666
6	85.21590604060837	2.8677315937947907	8.255253308482782	3.661109057114061
7	86.35329987287727	2.7860641083429982	6.108221446767994	4.7524145720117295
8	75.34667531033644	5.326999144074105	11.74378440796771	7.5825411376217415
9	49.68333274245747	21.43030788008934	14.29231641908968	14.59404295836351
10	40.51986102599685	20.93397215455287	25.11674018850628	13.429426630944
11	38.50172451341373	17.89733447456786	23.99909849225362	19.601842519764798
12	20.80988822316875	31.937431310680843	27.551697417534832	19.700983048615576
13	14.923371841557481	34.908481769791386	29.624532152932183	20.54361423571895
14	14.776623600257285	25.390864383859974	39.98870582991891	19.843806185963828
15	16.36894964218246	18.513575795023474	44.543599041768175	20.573875521025894
16	16.014677356455152	21.92018617654358	28.30101242358709	33.764124043414185
17	19.17894422300668	24.59090287520195	30.375239937806093	25.85491296398527
18	23.671668852907363	27.891662066276012	26.26375685627029	22.172912224546334
19	28.202884824788427	22.26470901051928	24.938717731847028	24.59368843284527
20	21.385105876513695	29.480822701788327	27.015984036222378	22.118087385475597
21	20.981579867002285	23.628745941948978	26.407213074901115	28.982461116147622
22	20.16363884991922	28.901553328234918	23.16988862834077	27.764919193505094
23	19.924207508850476	21.974124701818713	35.8322739773205	22.269393812010314
24	20.80786236306452	21.915248142539518	31.634945073867925	25.64194442052804
25	18.94014596322051	30.43361003205924	27.027632731821704	23.59861127289855
26	20.259487356100625	28.33684482418067	25.949495307601534	25.454172512117175
27	23.54492598013644	26.33276271607064	31.58429857126216	18.538012732530756
28	20.235936732388947	24.55646325343003	30.010711735301122	25.196888278879904
29	24.01327951298323	26.554214548714338	26.48736116527473	22.9451447730277
30	29.256332078988283	26.034074966953156	21.823451356566572	22.886141597491985
31	30.503755438168216	25.708417955198104	23.018582201806055	20.769244404827624
32	26.753255303954987	28.974990757013273	23.33562930811813	20.936124630913614
33	21.65821714181527	27.666158513423856	26.373026685642227	24.30259765911865
34	23.081257248780684	23.740674712707712	30.316489994783407	22.86157804372819
35	27.620450044822153	27.3677239968194	23.82905285975477	21.182773098603676
36	23.5463187589581	28.10615000481142	28.050692084458106	20.296839151772375
37	22.470333811098676	29.16909347824986	27.517890877045488	20.84268183360598
38	20.91080137961073	28.151098775874033	26.317821997801943	24.620277846713297
39	25.983934929373454	23.684963559841375	28.098932878190098	22.232168632595076
40	17.82098487188967	22.27711740365769	32.75435939771179	27.147538326740843
41	22.940966436562725	25.944304041084443	24.14344102468004	26.97128849767279
42	23.86298601650063	23.049856417165113	27.412926000395043	25.674231565939216
43	24.334251723247252	27.363925509123966	26.259198671035772	22.04262409659301
44	20.893581568724773	28.94460285544982	25.387825593703628	24.773989982121787
45	21.775083946578068	27.474841349830587	26.126504834208674	24.62356986938267
46	24.23308533429224	23.225346548694077	28.334692347819924	24.206875769193758
47	22.314215966816413	21.12706194573734	33.13180245838124	23.426919629065015
48	21.00538372322699	20.626168034966348	29.72455899557856	28.6438892462281
49	20.02030924754491	24.981514026548897	31.053903072723315	23.94427365318288
50	19.415463390175592	26.01027111072845	29.03956504783562	25.53470045126034
51	21.171124403004352	21.72621007156351	29.430556047952113	27.672109477480035
52	22.942865680410442	20.606922363976153	32.953653385465465	23.496558570147936
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134.0
1	246.5
2	359.0
3	1155.0
4	1951.0
5	1420.0
6	889.0
7	952.5
8	1016.0
9	1036.5
10	1057.0
11	1021.5
12	986.0
13	939.0
14	866.0
15	840.0
16	863.0
17	886.0
18	886.0
19	886.0
20	1035.0
21	1184.0
22	1407.5
23	1631.0
24	1763.0
25	1895.0
26	2464.5
27	3034.0
28	4323.5
29	5613.0
30	6053.0
31	6493.0
32	7320.0
33	8147.0
34	9925.5
35	11704.0
36	13234.5
37	14765.0
38	15667.0
39	19042.5
40	21516.0
41	31579.0
42	41642.0
43	57815.5
44	73989.0
45	87233.5
46	100478.0
47	99193.5
48	97909.0
49	86057.0
50	74205.0
51	65229.5
52	56254.0
53	49835.5
54	43417.0
55	39302.5
56	35188.0
57	32260.5
58	29333.0
59	29835.5
60	30338.0
61	30651.5
62	30965.0
63	30180.5
64	22811.5
65	16227.0
66	13141.5
67	10056.0
68	8091.5
69	6127.0
70	5150.0
71	4173.0
72	3647.0
73	3121.0
74	2545.0
75	1969.0
76	1740.5
77	1512.0
78	998.0
79	484.0
80	444.5
81	405.0
82	270.0
83	135.0
84	73.0
85	11.0
86	6.0
87	1.0
88	1.0
89	3.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	789788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.035709878265173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.82171568302059	18.178579327258408
2	12.125027553810218	6.313673993139482
3	4.601909691607879	3.5944195685003852
4	2.183140537627571	2.273584546446052
5	1.2350882153513842	1.6078199224476466
6	0.7578488143266763	1.1838679116837956
7	0.5892643218211586	1.0739340447183856
8	0.43792580857906427	0.9121367440295365
9	0.36450283846057197	0.8541081136767248
>10	7.3824076385037225	48.08439058015462
>50	0.3855145360713574	6.734288774333195
>100	0.10296654064552643	4.779078413777961
>500	0.007807889338049398	1.3428174865138713
>1k	0.004391937752652787	2.3735941227223147
>5k	4.8799308362808735E-4	0.6937064505975719
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	5460	0.691324760568659	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT	4004	0.5069714910836832	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	3363	0.4258104706579487	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3181	0.40276631197232676	No Hit
TGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTTG	2146	0.27171848647991614	Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1380	0.1747304339898808	No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1195	0.15130642653471565	No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	1182	0.14966041520002835	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1178	0.14915395017397073	No Hit
GCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1053	0.13332691810966993	No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	939	0.1188926648670276	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	897	0.11357478209342255	No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	869	0.11002952691101917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.518113721656951	0.0
2	0.0	0.0	0.0	1.920135530040973	0.0
3	0.0	0.0	0.0	2.9391431624689157	0.0
4	0.0	0.0	0.0	3.5628548420588815	0.0
5	0.0	0.0	0.0	6.743328589444256	0.0
6	0.0	0.0	0.0	7.232953653385465	0.0
7	0.0	0.0	0.0	8.988108201188167	0.0
8	0.0	0.0	0.0	11.058663843968255	0.0
9	0.0	0.0	0.0	12.50449487710626	0.0
10	0.0	0.0	0.0	13.643028255683804	0.0
11	0.0	0.0	0.0	14.720026133595345	0.0
12	0.0	0.0	0.0	15.71067172456406	0.0
13	0.0	0.0	0.0	16.13458295137429	0.0
14	0.0	0.0	0.0	16.449604197582136	0.0
15	0.0	0.0	0.0	17.429360790490612	0.0
16	0.0	0.0	0.0	18.44988781799673	0.0
17	0.0	0.0	0.0	19.773787396111363	0.0
18	0.0	0.0	0.0	20.44662618322892	0.0
19	0.0	0.0	0.0	21.13985018764529	0.0
20	0.0	0.0	0.0	22.014008822620752	0.0
21	1.266162565144064E-4	0.0	0.0	22.803081333218536	0.0
22	1.266162565144064E-4	0.0	0.0	23.72269520428267	0.0
23	1.266162565144064E-4	0.0	0.0	24.368311496249625	0.0
24	1.266162565144064E-4	0.0	0.0	24.918838979574264	0.0
25	1.266162565144064E-4	0.0	0.0	25.43302759727927	0.0
26	2.532325130288128E-4	0.0	0.0	25.895430166069882	1.266162565144064E-4
27	2.532325130288128E-4	0.0	0.0	26.406959842388083	1.266162565144064E-4
28	2.532325130288128E-4	0.0	0.0	26.872021352565497	1.266162565144064E-4
29	2.532325130288128E-4	0.0	0.0	27.47294210598287	1.266162565144064E-4
30	2.532325130288128E-4	0.0	0.0	28.059301989901087	1.266162565144064E-4
31	2.532325130288128E-4	0.0	0.0	28.595775068752626	1.266162565144064E-4
32	3.798487695432192E-4	0.0	0.0	29.092490643058643	1.266162565144064E-4
33	3.798487695432192E-4	0.0	0.0	29.57451873160899	1.266162565144064E-4
34	3.798487695432192E-4	0.0	0.0	30.20190228263787	1.266162565144064E-4
35	3.798487695432192E-4	0.0	0.0	30.69887108945692	1.266162565144064E-4
36	3.798487695432192E-4	0.0	0.0	31.239902353542977	1.266162565144064E-4
37	3.798487695432192E-4	0.0	0.0	31.722563523375893	1.266162565144064E-4
38	3.798487695432192E-4	0.0	0.0	32.18661210350119	1.266162565144064E-4
39	3.798487695432192E-4	0.0	0.0	32.74701565483396	1.266162565144064E-4
40	3.798487695432192E-4	0.0	0.0	33.24714986806586	1.266162565144064E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	35	1.01903424E-7	46.000004	27
TCGGGTA	35	1.01903424E-7	46.000004	4
GTTACGG	35	1.01903424E-7	46.000004	1
ATCTCGG	45	3.092282E-10	46.0	1
CGAACTA	20	6.31025E-4	46.0	35
CGTATTT	20	6.31025E-4	46.0	22
ATTACGG	25	3.4161778E-5	46.0	1
ACGGGTA	60	0.0	46.0	4
GCGATAT	20	6.31025E-4	46.0	8
CGTGTTT	25	3.4161778E-5	46.0	33
CCGAACT	20	6.31025E-4	46.0	34
GCTACGA	85	0.0	46.0	10
CGCCGTT	20	6.31025E-4	46.0	45
ATGCCGG	20	6.31025E-4	46.0	1
CCGTTGG	20	6.31025E-4	46.0	1
ATAGCGG	130	0.0	44.230766	1
CGTTGAT	110	0.0	43.909092	24
ATCGAGG	110	0.0	43.909092	1
GTCGGCG	480	0.0	43.604168	3
ACATACG	275	0.0	43.490906	16
>>END_MODULE