FastQCFastQC Report
Thu 26 May 2016
SRR1544870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544870_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1442567
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT69480.48164140729685345No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT49780.3450792926775671Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT39990.2772141605901147No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25270.17517383941265813No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24230.16796446889468564No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC17090.11846936745398999No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA16840.116736345694862No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT15360.10647685688082426No Hit
ACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC15180.1052290812142521No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG206.3119916E-446.00000427
CAGGTCG206.3119916E-446.0000044
CGAACGC206.3119916E-446.00000410
TACTTCG206.3119916E-446.00000428
CGAGTAA206.3119916E-446.00000417
CGGCAAC206.3119916E-446.00000444
CGCGCGA206.3119916E-446.00000428
TGTATCG206.3119916E-446.00000414
CCGTAGA206.3119916E-446.00000424
TACGATA206.3119916E-446.00000413
CTACGAT206.3119916E-446.00000412
AGATTCG206.3119916E-446.00000431
CCCTTAT206.3119916E-446.00000413
CGAGCTA206.3119916E-446.00000430
TCGTACC405.6134013E-946.00000429
ACCCGTA206.3119916E-446.00000421
GCCCGTA206.3119916E-446.00000422
TGCGTAA206.3119916E-446.00000414
GTTGCGT206.3119916E-446.00000412
CGCATTG253.417592E-546.027
Produced by FastQC (version 0.11.5)