##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544870_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1442567
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.911330288298565	34.0	33.0	34.0	31.0	34.0
2	33.23741982174831	34.0	33.0	34.0	31.0	34.0
3	33.29223322036342	34.0	34.0	34.0	31.0	34.0
4	36.57218624854166	37.0	37.0	37.0	35.0	37.0
5	36.56814553500808	37.0	37.0	37.0	35.0	37.0
6	36.67423003576264	37.0	37.0	37.0	35.0	37.0
7	36.64734324298282	37.0	37.0	37.0	35.0	37.0
8	36.55018796354	37.0	37.0	37.0	35.0	37.0
9	38.33582634290123	39.0	39.0	39.0	37.0	39.0
10	38.09365457548939	39.0	39.0	39.0	35.0	39.0
11	37.25072457639749	39.0	35.0	39.0	35.0	39.0
12	37.13706261130332	39.0	35.0	39.0	35.0	39.0
13	37.11745173707703	39.0	35.0	39.0	35.0	39.0
14	38.33901233010321	40.0	35.0	41.0	35.0	41.0
15	38.41850534498571	40.0	35.0	41.0	35.0	41.0
16	38.41526736713095	40.0	35.0	41.0	35.0	41.0
17	38.49400270490036	40.0	36.0	41.0	35.0	41.0
18	38.46977090145553	40.0	36.0	41.0	35.0	41.0
19	38.375438367853974	40.0	35.0	41.0	35.0	41.0
20	38.28426201348014	40.0	35.0	41.0	35.0	41.0
21	38.222737661404985	40.0	35.0	41.0	35.0	41.0
22	38.19752496764448	40.0	35.0	41.0	35.0	41.0
23	38.16144761387166	40.0	35.0	41.0	35.0	41.0
24	38.108541925609	40.0	35.0	41.0	35.0	41.0
25	38.08007600340227	40.0	35.0	41.0	35.0	41.0
26	38.022833601489566	40.0	35.0	41.0	35.0	41.0
27	37.99750028941463	40.0	35.0	41.0	35.0	41.0
28	38.04521107165213	40.0	35.0	41.0	35.0	41.0
29	38.04743696479956	40.0	35.0	41.0	35.0	41.0
30	37.958608508304984	40.0	35.0	41.0	35.0	41.0
31	37.90561755537178	40.0	35.0	41.0	35.0	41.0
32	37.82101004667374	40.0	35.0	41.0	35.0	41.0
33	37.754739987813394	40.0	35.0	41.0	35.0	41.0
34	37.67304256925328	40.0	35.0	41.0	35.0	41.0
35	37.602346372820115	40.0	35.0	41.0	35.0	41.0
36	37.5144960338064	40.0	35.0	41.0	35.0	41.0
37	37.443288942558645	39.0	35.0	41.0	34.0	41.0
38	37.40770238054801	39.0	35.0	41.0	35.0	41.0
39	37.276708811445154	39.0	35.0	41.0	34.0	41.0
40	37.18208720981417	39.0	35.0	41.0	34.0	41.0
41	37.16299693532432	39.0	35.0	41.0	34.0	41.0
42	37.06224390270955	38.0	35.0	41.0	34.0	41.0
43	37.06246711591212	38.0	35.0	41.0	34.0	41.0
44	37.02911060630113	38.0	35.0	41.0	34.0	41.0
45	36.99039490020221	38.0	35.0	41.0	34.0	41.0
46	36.94067311951542	38.0	35.0	41.0	34.0	41.0
47	36.847071921096216	37.0	35.0	41.0	34.0	41.0
48	36.837028713397714	37.0	35.0	41.0	34.0	41.0
49	36.7480200226402	37.0	35.0	41.0	34.0	41.0
50	36.64492255818967	37.0	35.0	41.0	34.0	41.0
51	36.535176529062426	36.0	35.0	40.0	34.0	41.0
52	36.22445404615522	36.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	9.0
15	11.0
16	33.0
17	71.0
18	106.0
19	213.0
20	418.0
21	684.0
22	1123.0
23	1860.0
24	2888.0
25	4756.0
26	6633.0
27	7937.0
28	7991.0
29	8978.0
30	10509.0
31	13079.0
32	16786.0
33	24498.0
34	81471.0
35	346931.0
36	52175.0
37	81462.0
38	139586.0
39	627159.0
40	5198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.00470619388909	22.347870150918467	23.47537410740714	21.172049547785303
2	26.586217485912268	24.869208847838607	26.067905338192265	22.47666832805686
3	26.58115706237561	25.3941064782433	25.655099555167975	22.369636904213113
4	26.532493811379297	26.540812315823114	23.378324888895975	23.548368983901614
5	24.27741657753158	34.85869287180422	20.94121104946945	19.922679501194747
6	87.57464991227444	3.021211493123023	6.836978802371051	2.5671597922314873
7	88.27860335083223	2.7620207588278394	5.268594110360212	3.6907817799797167
8	79.45939426037057	5.072762651578748	9.46874564578283	5.999097442267846
9	54.553861276460644	20.00870670131786	12.939502983223655	12.497929038997842
10	46.53399114217919	19.614617553292153	21.378695062343724	12.472696242184938
11	43.62833753995481	18.078882991223285	21.364900209141066	16.927879259680832
12	20.565977178183058	37.83595493311576	24.48142789901613	17.116639989685055
13	14.868148238522025	39.47366049549172	26.69241705931163	18.96577420667463
14	13.382740628338233	24.097112993711903	44.34490737691906	18.175239001030803
15	15.319981671561875	17.07809758576205	48.03305496382491	19.568865778851173
16	16.562419631115922	20.17320512669429	26.372570563446967	36.89180467874282
17	19.53198707581693	23.08620674117736	31.91089218039786	25.470914002607852
18	25.99352404429049	26.114766246559086	25.606574945912392	22.28513476323803
19	30.019888157707754	22.35889216930652	23.999370566497085	23.62184910648864
20	21.6023241901416	29.27649114391221	27.25384678839874	21.867337877547456
21	21.618753236418133	24.18140717207589	25.119595831597426	29.08024375990855
22	19.24375089683876	30.069313938278086	22.58376907277097	28.103166092112186
23	19.765251804595557	21.871913054991555	37.744797988585624	20.618037151827266
24	19.077450128832837	21.7666839737773	34.432230877318005	24.723635020071857
25	17.057439966393243	32.719312170595884	26.921037289775796	23.302210573235076
26	18.619724421811952	30.799262703222794	26.838198849689476	23.742814025275777
27	19.11245716836722	27.172949332682638	35.66836063766882	18.046232861281315
28	15.940472782200063	25.46113975988637	31.885243458362766	26.713143999550798
29	20.523552805519603	28.604494626592736	27.414601886775447	23.457350681112214
30	26.42137245618401	27.988370730787548	21.694798231208672	23.89545858181977
31	28.279379744580318	27.056559591339607	22.387521688767315	22.27653897531276
32	25.905001292834235	31.093737760533823	22.24395816624115	20.75730278039079
33	17.760422912765925	29.0229154001166	24.657641551484264	28.55902013563322
34	18.515951078875368	28.191966127049906	27.522742444545035	25.769340349529692
35	27.285457105285232	26.460330785329205	23.077333669770624	23.17687843961494
36	19.940425644008215	32.68804845806122	24.838153097915036	22.533372800015528
37	19.948813469322396	32.07982714147766	24.468395575387486	23.50296381381246
38	18.62686447145956	31.83762002042193	24.63324060511574	24.902274903002773
39	25.624667693077686	26.305259998322434	24.53154688829011	23.538525420309767
40	16.246593745732437	24.32656507462045	29.598278624147095	29.82856255550002
41	21.49522344542749	27.724396856437174	22.325618151531263	28.454761546604075
42	21.641767765379356	26.37915604613165	25.747920200586872	26.23115598790212
43	21.745194503964115	28.747919507378167	25.914983498166812	23.59190249049091
44	17.83133816314944	33.09482332536374	22.891137811969912	26.182700699516904
45	21.346946103716498	29.74676392846918	24.089279735360645	24.817010232453672
46	22.596940038140342	27.03631789719299	25.399998752224334	24.966743312442333
47	21.2543334209087	23.634742788376553	30.621662633347356	24.489261157367388
48	19.06448712607456	22.549663204551333	28.902366406551653	29.483483262822457
49	19.325618844739967	28.00577026924919	28.833461461408728	23.835149424602115
50	17.754946564007078	29.67231331369704	25.810378304785843	26.76236181751004
51	19.757071941892473	24.73763783588561	27.882171157388186	27.62311906483373
52	22.83900851745534	22.585432773659733	29.513152595338727	25.0624061135462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	231.0
1	516.5
2	802.0
3	2769.5
4	4737.0
5	3288.0
6	1839.0
7	2051.5
8	2264.0
9	2299.0
10	2334.0
11	2210.0
12	2086.0
13	2026.5
14	1936.5
15	1906.0
16	1868.5
17	1831.0
18	1899.0
19	1967.0
20	1913.5
21	1860.0
22	2198.0
23	2536.0
24	3190.5
25	3845.0
26	4834.0
27	5823.0
28	8323.5
29	10824.0
30	11638.0
31	12452.0
32	14383.5
33	16315.0
34	19656.0
35	22997.0
36	23907.5
37	24818.0
38	28021.5
39	38255.0
40	45285.0
41	65735.0
42	86185.0
43	117477.5
44	148770.0
45	165557.5
46	182345.0
47	178291.0
48	174237.0
49	152340.0
50	130443.0
51	116050.0
52	101657.0
53	89530.5
54	77404.0
55	68942.0
56	60480.0
57	57771.5
58	55063.0
59	52450.0
60	49837.0
61	52634.5
62	55432.0
63	49394.0
64	34832.0
65	26308.0
66	21454.5
67	16601.0
68	13999.5
69	11398.0
70	10143.5
71	8889.0
72	7069.0
73	5249.0
74	4434.5
75	3620.0
76	3085.0
77	2550.0
78	2021.5
79	1493.0
80	1167.5
81	842.0
82	576.0
83	310.0
84	190.5
85	71.0
86	62.0
87	53.0
88	39.5
89	14.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1442567.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.931601057971243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.97142590672745	13.547793445143427
2	12.790524480535032	5.098712625364783
3	5.514239905548677	3.297228898060238
4	2.8968236143059976	2.3095333046263
5	1.7128781409282712	1.7070201882950873
6	1.1140825102949758	1.332326888451756
7	0.7485326685147777	1.0443618169386628
8	0.5286201086012503	0.8428996092689243
9	0.40987270227925987	0.7352477267744545
>10	3.4469959055611925	16.620843924572075
>50	2.291826629281191	33.37133251397035
>100	0.5595264845594212	16.415860558970948
>500	0.008720799322923026	1.1419940690831076
>1k	0.005581311566670737	2.051765295503349
>5k	3.4883197291692104E-4	0.4830791349765255
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	6948	0.48164140729685345	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT	4978	0.3450792926775671	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	3999	0.2772141605901147	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2527	0.17517383941265813	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2423	0.16796446889468564	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1709	0.11846936745398999	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1684	0.116736345694862	No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	1536	0.10647685688082426	No Hit
ACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1518	0.1052290812142521	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3538830432139374	0.0
2	0.0	0.0	0.0	1.3089859951045602	0.0
3	6.932087036511996E-5	0.0	0.0	2.0250705859762492	0.0
4	6.932087036511996E-5	0.0	0.0	2.4553452283325488	0.0
5	6.932087036511996E-5	0.0	0.0	4.7712168654904765	0.0
6	6.932087036511996E-5	0.0	0.0	5.129189840055956	0.0
7	1.3864174073023993E-4	0.0	0.0	6.4803229243425085	0.0
8	1.3864174073023993E-4	0.0	0.0	8.117057994533356	0.0
9	1.3864174073023993E-4	0.0	0.0	9.203870600117707	0.0
10	1.3864174073023993E-4	0.0	0.0	10.097277977383373	0.0
11	1.3864174073023993E-4	0.0	0.0	10.960184171688386	0.0
12	1.3864174073023993E-4	0.0	0.0	11.780527351589216	0.0
13	1.3864174073023993E-4	0.0	0.0	12.105434270990532	0.0
14	1.3864174073023993E-4	0.0	0.0	12.341818438935592	0.0
15	1.3864174073023993E-4	0.0	0.0	13.17242110765046	0.0
16	1.3864174073023993E-4	0.0	0.0	14.018413009586384	0.0
17	1.3864174073023993E-4	0.0	0.0	15.225220041772756	0.0
18	1.3864174073023993E-4	0.0	0.0	15.759545310547102	0.0
19	1.3864174073023993E-4	0.0	0.0	16.334215325873945	0.0
20	1.3864174073023993E-4	0.0	0.0	17.154558505774776	0.0
21	1.3864174073023993E-4	0.0	0.0	17.821633241298326	0.0
22	1.3864174073023993E-4	0.0	0.0	18.641144570754772	0.0
23	1.3864174073023993E-4	0.0	0.0	19.24305768813511	0.0
24	1.3864174073023993E-4	0.0	0.0	19.72955155635752	0.0
25	1.3864174073023993E-4	0.0	0.0	20.221105848116586	0.0
26	1.3864174073023993E-4	0.0	0.0	20.64396315734382	0.0
27	1.3864174073023993E-4	0.0	0.0	21.083041550236487	0.0
28	1.3864174073023993E-4	0.0	0.0	21.523645002277192	0.0
29	1.3864174073023993E-4	0.0	0.0	22.11446678039911	0.0
30	1.3864174073023993E-4	0.0	0.0	22.684145693059662	0.0
31	1.3864174073023993E-4	0.0	0.0	23.159409580282926	0.0
32	1.3864174073023993E-4	0.0	0.0	23.62642428393274	0.0
33	1.3864174073023993E-4	0.0	0.0	24.058501268918533	0.0
34	1.3864174073023993E-4	0.0	0.0	24.712335718202343	0.0
35	1.3864174073023993E-4	0.0	0.0	25.180112951426175	0.0
36	1.3864174073023993E-4	0.0	0.0	25.68456092507315	0.0
37	1.3864174073023993E-4	0.0	0.0	26.131056651094887	0.0
38	1.3864174073023993E-4	0.0	0.0	26.54822964895218	0.0
39	1.3864174073023993E-4	0.0	0.0	27.036941785026276	0.0
40	1.3864174073023993E-4	0.0	0.0	27.537507789932807	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	20	6.3119916E-4	46.000004	27
CAGGTCG	20	6.3119916E-4	46.000004	4
CGAACGC	20	6.3119916E-4	46.000004	10
TACTTCG	20	6.3119916E-4	46.000004	28
CGAGTAA	20	6.3119916E-4	46.000004	17
CGGCAAC	20	6.3119916E-4	46.000004	44
CGCGCGA	20	6.3119916E-4	46.000004	28
TGTATCG	20	6.3119916E-4	46.000004	14
CCGTAGA	20	6.3119916E-4	46.000004	24
TACGATA	20	6.3119916E-4	46.000004	13
CTACGAT	20	6.3119916E-4	46.000004	12
AGATTCG	20	6.3119916E-4	46.000004	31
CCCTTAT	20	6.3119916E-4	46.000004	13
CGAGCTA	20	6.3119916E-4	46.000004	30
TCGTACC	40	5.6134013E-9	46.000004	29
ACCCGTA	20	6.3119916E-4	46.000004	21
GCCCGTA	20	6.3119916E-4	46.000004	22
TGCGTAA	20	6.3119916E-4	46.000004	14
GTTGCGT	20	6.3119916E-4	46.000004	12
CGCATTG	25	3.417592E-5	46.0	27
>>END_MODULE