FastQCFastQC Report
Thu 26 May 2016
SRR1544869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544869_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1031947
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT78280.7585660891499273No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT56740.5498344391717791Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT42690.41368403609875315No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA35880.3476922748939626No Hit
TGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTTG28020.27152557253424836Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC16950.1642526215009104No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC16630.1611516870536956No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT16530.16018264503894095No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15820.15330244673418306No Hit
GCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC14400.13954205012466725No Hit
AGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC12680.12287452747088755No Hit
ACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT11340.10988936447317546No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.10591629221268145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT1400.046.0000045
ACCGGGT351.0194344E-746.0000043
GTCGATC206.311152E-446.0000047
CCGATTA405.6097633E-946.00000411
ATAGACG206.311152E-446.00000422
ACGATAT206.311152E-446.00000414
TACGATA206.311152E-446.00000413
CTACGAT206.311152E-446.00000412
AACGCTA351.0194344E-746.00000432
ACGCTAT206.311152E-446.00000441
ACGCTAG351.0194344E-746.00000433
TCGTAAG206.311152E-446.00000433
CTATTCG206.311152E-446.00000444
GACGCTA206.311152E-446.00000440
ACGTAGG351.0194344E-746.0000041
TCGGTTA206.311152E-446.00000436
ATACCCC206.311152E-446.0000049
GTACGTC206.311152E-446.00000412
TAGTAAG206.311152E-446.00000430
GCGACTA253.416911E-546.027
Produced by FastQC (version 0.11.5)