##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544869_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1031947
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91460607957579	34.0	33.0	34.0	31.0	34.0
2	33.2596150771309	34.0	33.0	34.0	31.0	34.0
3	33.35182523908689	34.0	34.0	34.0	31.0	34.0
4	36.58324313167246	37.0	37.0	37.0	35.0	37.0
5	36.58654078164867	37.0	37.0	37.0	35.0	37.0
6	36.52278653845595	37.0	37.0	37.0	35.0	37.0
7	36.40314764227232	37.0	37.0	37.0	35.0	37.0
8	36.442462645852935	37.0	37.0	37.0	35.0	37.0
9	38.281072574463614	39.0	39.0	39.0	37.0	39.0
10	38.11449715925333	39.0	39.0	39.0	35.0	39.0
11	37.37189797538052	39.0	35.0	39.0	35.0	39.0
12	37.310774681257854	39.0	35.0	39.0	35.0	39.0
13	37.293328048824215	39.0	35.0	39.0	35.0	39.0
14	38.49703424691384	41.0	35.0	41.0	35.0	41.0
15	38.574528536833775	41.0	35.0	41.0	35.0	41.0
16	38.58821237912412	41.0	35.0	41.0	35.0	41.0
17	38.66619215909344	41.0	36.0	41.0	35.0	41.0
18	38.653630467456175	40.0	36.0	41.0	35.0	41.0
19	38.56988973270914	40.0	35.0	41.0	35.0	41.0
20	38.49311737909021	40.0	35.0	41.0	35.0	41.0
21	38.43794497198015	40.0	35.0	41.0	35.0	41.0
22	38.41996245931235	40.0	35.0	41.0	35.0	41.0
23	38.39367913274616	40.0	35.0	41.0	35.0	41.0
24	38.328261044414106	40.0	35.0	41.0	35.0	41.0
25	38.277543323445876	40.0	35.0	41.0	35.0	41.0
26	38.22531292789262	40.0	35.0	41.0	35.0	41.0
27	38.21133837299784	40.0	35.0	41.0	35.0	41.0
28	38.28090202306901	40.0	35.0	41.0	35.0	41.0
29	38.32568242361284	40.0	35.0	41.0	35.0	41.0
30	38.249939192613574	40.0	35.0	41.0	35.0	41.0
31	38.22671513168797	40.0	35.0	41.0	35.0	41.0
32	38.164815634911484	40.0	35.0	41.0	35.0	41.0
33	38.11512219135285	40.0	35.0	41.0	35.0	41.0
34	38.048028629377285	40.0	35.0	41.0	35.0	41.0
35	37.96605930343322	40.0	35.0	41.0	35.0	41.0
36	37.926164812727784	40.0	35.0	41.0	35.0	41.0
37	37.86556092512503	40.0	35.0	41.0	35.0	41.0
38	37.80429615086821	40.0	35.0	41.0	35.0	41.0
39	37.74906172506921	40.0	35.0	41.0	35.0	41.0
40	37.682123209816005	40.0	35.0	41.0	35.0	41.0
41	37.659587168720876	40.0	35.0	41.0	35.0	41.0
42	37.59168930187306	39.0	35.0	41.0	35.0	41.0
43	37.51836383070061	39.0	35.0	41.0	35.0	41.0
44	37.38428814658117	39.0	35.0	41.0	35.0	41.0
45	37.35452983535007	39.0	35.0	41.0	35.0	41.0
46	37.291312441433526	39.0	35.0	41.0	35.0	41.0
47	37.20069538454979	38.0	35.0	41.0	35.0	41.0
48	37.158953899764235	38.0	35.0	41.0	35.0	41.0
49	37.0852291832817	38.0	35.0	41.0	35.0	41.0
50	36.9816172729801	37.0	35.0	41.0	34.0	41.0
51	36.86096378980704	37.0	35.0	40.0	34.0	41.0
52	36.37348914236875	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	6.0
14	6.0
15	18.0
16	29.0
17	46.0
18	87.0
19	154.0
20	267.0
21	429.0
22	695.0
23	1090.0
24	1675.0
25	2677.0
26	3827.0
27	4494.0
28	4532.0
29	4900.0
30	5786.0
31	7337.0
32	9618.0
33	15006.0
34	50636.0
35	236238.0
36	35039.0
37	57771.0
38	102542.0
39	483930.0
40	3107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.632729200239933	21.991051866035754	23.08035199482144	23.295866938902872
2	26.03554252301717	25.113789758582563	26.434206407887228	22.41646131051304
3	26.76009523744921	25.220675092809998	25.56991783492757	22.44931183481322
4	26.67355978553162	25.941448543384492	23.818858914265945	23.566132756817936
5	24.146298211051537	33.864045343413956	21.090133504918374	20.899522940616137
6	85.0994285559239	3.0792279060843244	8.2237750582152	3.597568479776578
7	86.13698184112168	2.7357025118538063	6.245669593496565	4.881646053527943
8	75.81309892853024	5.176041017610401	11.53441019742293	7.476449856436425
9	52.52663169717049	19.24381775420637	14.057407987038095	14.172142561585044
10	44.11408725448109	19.011635287471158	23.92942660814945	12.944850849898298
11	40.77932296910597	17.896558641093	22.3297320501925	18.994386339608525
12	19.917398858662317	35.544945622207344	26.459304596069373	18.07835092306097
13	14.771688856113736	37.456865517318235	28.09320633714716	19.67823928942087
14	13.12596480245594	24.253377353681923	42.926235552794864	19.694422291067273
15	14.688157434441884	17.224334195457715	47.437513748283585	20.649994621816816
16	15.940934951116676	21.584151124040286	26.515799745529566	35.959114179313474
17	18.433504821468542	23.294316471679263	31.911716396287794	26.360462310564397
18	24.685473188061014	26.49438391700349	26.399611607960487	22.42053128697501
19	28.344769644177465	22.07061021544711	24.39912127270102	25.185498867674404
20	20.538942406925937	30.022859701128063	27.5523839887126	21.8858139032334
21	20.995458100076846	24.06422035240182	25.52718308207689	29.413138465444444
22	18.544363227956477	30.195155371351433	22.68469214019712	28.575789260494965
23	18.816664034102526	21.887655083061436	37.92249020540784	21.373190677428198
24	19.889490448637382	21.854320037753876	33.324773462203	24.93141605140574
25	17.156307446021938	32.059398399336395	26.784127479415126	24.000166675226538
26	18.915700128010453	29.680206444710823	26.04067844569537	25.363414981583354
27	20.36034796360666	26.97454423531441	34.09874731938753	18.566360481691405
28	16.103927817998407	25.430957210011755	30.379467162557766	28.085647809432075
29	21.49112309062384	28.147085073167517	25.958116066038272	24.403675770170366
30	25.29238420190184	29.13386055679216	20.7610468367077	24.8127084045983
31	27.770224633629443	26.738679408923133	21.059705585655077	24.43139037179235
32	24.931706764010166	32.272587642582415	21.60004341308226	21.195662180325154
33	17.32094768432875	28.83743060447872	23.9924143391085	29.84920737208403
34	18.577213752256657	28.66746063509076	26.854189217081885	25.901136395570703
35	26.08380081535195	27.276691535514907	22.58245820764051	24.057049441492634
36	20.080876246551423	33.46402479972324	23.114462273740802	23.340636679984534
37	19.693162536448092	32.50670819334714	24.72617295268071	23.073956317524058
38	18.070598586942936	32.204948509952544	23.381142636201275	26.34331026690324
39	26.010638143237973	26.359105651743743	24.199498617661565	23.430757587356716
40	16.25907144456062	24.269366546925376	29.325827779915052	30.145734228598947
41	21.247118311308625	28.239434777173635	21.61438523490063	28.89906167661711
42	20.87297118941186	25.869351817486752	26.22227691926039	27.035400073841004
43	22.636530752063816	27.680588247264637	25.39859120671895	24.284289793952595
44	17.900531713353494	31.36052529829536	23.69356178175817	27.04538120659297
45	20.752616171179334	28.58518896803809	24.213937343681412	26.44825751710117
46	22.947302526195628	25.644146453257772	25.44384546880799	25.96470555173861
47	19.951315329178726	23.02569802518928	31.793977791495102	25.229008854136886
48	18.74272612837675	21.67931104988919	28.487897149756726	31.090065671977342
49	19.166197488824523	26.517544021156127	29.678849785890165	24.637408704129186
50	17.90838095367301	27.743769786626636	26.92463857155455	27.423210688145804
51	19.519897824209963	23.369223419419797	28.0492118296773	29.061666926692943
52	22.16421967407241	21.806449362224996	31.248213328785297	24.781117634917297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	272.5
2	440.0
3	1362.5
4	2285.0
5	1672.5
6	1060.0
7	1157.0
8	1254.0
9	1278.0
10	1302.0
11	1308.5
12	1315.0
13	1263.5
14	1209.5
15	1207.0
16	1205.0
17	1203.0
18	1201.5
19	1200.0
20	1227.0
21	1254.0
22	1441.0
23	1628.0
24	2044.0
25	2460.0
26	3027.0
27	3594.0
28	5009.0
29	6424.0
30	6954.0
31	7484.0
32	8762.5
33	10041.0
34	12283.5
35	14526.0
36	15239.5
37	15953.0
38	18070.0
39	24708.0
40	29229.0
41	43570.5
42	57912.0
43	81862.0
44	105812.0
45	122828.0
46	139844.0
47	138431.0
48	137018.0
49	118668.0
50	100318.0
51	87528.5
52	74739.0
53	65158.5
54	55578.0
55	49023.5
56	42469.0
57	40248.0
58	38027.0
59	35721.0
60	33415.0
61	35165.5
62	36916.0
63	34061.0
64	24473.0
65	17740.0
66	14573.0
67	11406.0
68	9593.5
69	7781.0
70	7053.5
71	6326.0
72	4996.0
73	3666.0
74	3155.0
75	2644.0
76	2214.5
77	1785.0
78	1438.0
79	1091.0
80	828.0
81	565.0
82	392.5
83	220.0
84	145.0
85	70.0
86	50.5
87	31.0
88	17.5
89	2.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1031947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.15905257782287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.38916667046684	15.83828306674833
2	12.872223640496355	5.962170081674992
3	5.241027498247444	3.6413169418118403
4	2.7389817559341694	2.5372888998150818
5	1.544636885078636	1.7886163417590337
6	0.9944356897475483	1.381811305447621
7	0.644667447036504	1.0450921110790465
8	0.4899319835445214	0.9077088453171696
9	0.3762432581847066	0.7842093658515905
>10	5.048391815696277	32.34598422199798
>50	1.4345001752835798	20.709791092616538
>100	0.2106843429410506	8.034821951018731
>500	0.009652868302080007	1.55407021235863
>1k	0.004616589187951307	2.156492326074234
>5k	8.393798523547831E-4	1.312343236429184
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	7828	0.7585660891499273	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT	5674	0.5498344391717791	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	4269	0.41368403609875315	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3588	0.3476922748939626	No Hit
TGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTTG	2802	0.27152557253424836	Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1695	0.1642526215009104	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1663	0.1611516870536956	No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	1653	0.16018264503894095	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1582	0.15330244673418306	No Hit
GCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1440	0.13954205012466725	No Hit
AGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1268	0.12287452747088755	No Hit
ACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	1134	0.10988936447317546	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1093	0.10591629221268145	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5610753265429329	0.0
2	0.0	0.0	0.0	2.0314996797316143	0.0
3	0.0	0.0	0.0	3.1069425077063064	0.0
4	0.0	0.0	0.0	3.7460257164369875	0.0
5	0.0	0.0	0.0	7.062281299330295	0.0
6	0.0	0.0	0.0	7.5627914999510635	0.0
7	0.0	0.0	0.0	9.478199946315073	0.0
8	0.0	0.0	0.0	11.622205403959699	0.0
9	0.0	0.0	0.0	13.078481743732963	0.0
10	0.0	0.0	0.0	14.259259438711485	0.0
11	0.0	0.0	0.0	15.362513772509635	0.0
12	0.0	0.0	0.0	16.427103329919074	0.0
13	0.0	0.0	0.0	16.840496653413403	0.0
14	0.0	0.0	0.0	17.143806804031602	0.0
15	0.0	0.0	0.0	18.175448932939386	0.0
16	0.0	0.0	0.0	19.196043982878965	0.0
17	0.0	0.0	0.0	20.57140531442022	0.0
18	0.0	0.0	0.0	21.20593402568155	0.0
19	0.0	0.0	0.0	21.880193459547826	0.0
20	0.0	0.0	0.0	22.814737578577194	0.0
21	0.0	0.0	0.0	23.602084215565334	0.0
22	0.0	0.0	0.0	24.557462737911926	0.0
23	0.0	0.0	0.0	25.210887768460978	0.0
24	0.0	0.0	0.0	25.7546172429398	0.0
25	9.690420147546337E-5	0.0	0.0	26.296699345993545	0.0
26	9.690420147546337E-5	0.0	0.0	26.77589062228971	0.0
27	9.690420147546337E-5	0.0	0.0	27.260896150674405	0.0
28	9.690420147546337E-5	0.0	0.0	27.742413127805982	0.0
29	9.690420147546337E-5	0.0	0.0	28.373550192015674	0.0
30	9.690420147546337E-5	0.0	0.0	28.992574231040937	0.0
31	9.690420147546337E-5	0.0	0.0	29.50577888205499	0.0
32	9.690420147546337E-5	0.0	0.0	30.00793645410084	0.0
33	9.690420147546337E-5	0.0	0.0	30.44943199602305	0.0
34	9.690420147546337E-5	0.0	0.0	31.145688683624257	0.0
35	9.690420147546337E-5	0.0	0.0	31.631663254023703	0.0
36	9.690420147546337E-5	0.0	0.0	32.14137935378464	0.0
37	9.690420147546337E-5	0.0	0.0	32.58200275789358	0.0
38	9.690420147546337E-5	0.0	0.0	33.01545525109332	0.0
39	9.690420147546337E-5	0.0	0.0	33.504918372745884	0.0
40	9.690420147546337E-5	0.0	0.0	34.0084326036124	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	140	0.0	46.000004	5
ACCGGGT	35	1.0194344E-7	46.000004	3
GTCGATC	20	6.311152E-4	46.000004	7
CCGATTA	40	5.6097633E-9	46.000004	11
ATAGACG	20	6.311152E-4	46.000004	22
ACGATAT	20	6.311152E-4	46.000004	14
TACGATA	20	6.311152E-4	46.000004	13
CTACGAT	20	6.311152E-4	46.000004	12
AACGCTA	35	1.0194344E-7	46.000004	32
ACGCTAT	20	6.311152E-4	46.000004	41
ACGCTAG	35	1.0194344E-7	46.000004	33
TCGTAAG	20	6.311152E-4	46.000004	33
CTATTCG	20	6.311152E-4	46.000004	44
GACGCTA	20	6.311152E-4	46.000004	40
ACGTAGG	35	1.0194344E-7	46.000004	1
TCGGTTA	20	6.311152E-4	46.000004	36
ATACCCC	20	6.311152E-4	46.000004	9
GTACGTC	20	6.311152E-4	46.000004	12
TAGTAAG	20	6.311152E-4	46.000004	30
GCGACTA	25	3.416911E-5	46.0	27
>>END_MODULE