FastQCFastQC Report
Thu 26 May 2016
SRR1544868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544868_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences721613
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT46560.6452211919685482Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT36120.500545306140549Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT31210.43250329470228505Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT13910.19276260266929782Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC12080.1674027491189876No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC11740.16269108233914856No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.15770225868990717No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC10740.14883323886903368No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8460.11723735575717177No Hit
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT8120.11252568897733273Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC8000.11086274776091895No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT7410.10268662011355116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA700.046.0000044
CTCGAGG1450.046.0000041
TGTCCAA351.0188705E-746.00000434
GTCATAG206.3098845E-446.041
CGGGTCA405.6061253E-946.05
TGTCAAC206.3098845E-446.033
CGGGTAC206.3098845E-446.05
ATCTCGG301.8604333E-646.01
AAATCGT206.3098845E-446.011
ACGCCAT206.3098845E-446.042
CATGCGG1550.046.01
CCGGATA206.3098845E-446.019
CACGCTC253.415883E-546.018
GGTCATA253.415883E-546.07
CGATTGC301.8604333E-646.09
CGATTCC206.3098845E-446.09
ATCACGG206.3098845E-446.01
CTAACGA253.415883E-546.019
CGCTCAT206.3098845E-446.020
TGCGCAC253.415883E-546.021
Produced by FastQC (version 0.11.5)