##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544868_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	721613
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86520752813489	34.0	31.0	34.0	31.0	34.0
2	33.172603597773325	34.0	33.0	34.0	31.0	34.0
3	33.2164331851006	34.0	33.0	34.0	31.0	34.0
4	36.528578337696246	37.0	37.0	37.0	35.0	37.0
5	36.512075031907685	37.0	37.0	37.0	35.0	37.0
6	36.645018867453885	37.0	37.0	37.0	35.0	37.0
7	36.625394775315854	37.0	37.0	37.0	35.0	37.0
8	36.536165506996134	37.0	37.0	37.0	35.0	37.0
9	38.29078051531777	39.0	39.0	39.0	37.0	39.0
10	38.0487117055818	39.0	39.0	39.0	35.0	39.0
11	37.149923851150135	39.0	35.0	39.0	35.0	39.0
12	37.014061553769125	39.0	35.0	39.0	35.0	39.0
13	36.987127449200614	39.0	35.0	39.0	35.0	39.0
14	38.208462153536594	40.0	35.0	41.0	35.0	41.0
15	38.29749741204773	40.0	35.0	41.0	35.0	41.0
16	38.30557099165342	40.0	35.0	41.0	35.0	41.0
17	38.39731407277862	40.0	36.0	41.0	35.0	41.0
18	38.37470777272583	40.0	36.0	41.0	35.0	41.0
19	38.25520604534564	40.0	35.0	41.0	35.0	41.0
20	38.164006191684464	40.0	35.0	41.0	35.0	41.0
21	38.1076491138602	40.0	35.0	41.0	35.0	41.0
22	38.09786409058595	40.0	35.0	41.0	35.0	41.0
23	38.06495586969747	40.0	35.0	41.0	35.0	41.0
24	38.03019901249007	40.0	35.0	41.0	35.0	41.0
25	37.995503129793946	40.0	35.0	41.0	35.0	41.0
26	37.956403224443015	40.0	35.0	41.0	35.0	41.0
27	37.945229645253065	40.0	35.0	41.0	35.0	41.0
28	37.98550192416157	40.0	35.0	41.0	35.0	41.0
29	37.982788558410114	40.0	35.0	41.0	35.0	41.0
30	37.882413426587384	40.0	35.0	41.0	35.0	41.0
31	37.814005568081505	40.0	35.0	41.0	35.0	41.0
32	37.731953276894956	40.0	35.0	41.0	35.0	41.0
33	37.60884296707515	40.0	35.0	41.0	35.0	41.0
34	37.53944150119247	40.0	35.0	41.0	35.0	41.0
35	37.46895219459738	39.0	35.0	41.0	35.0	41.0
36	37.360578315523696	39.0	35.0	41.0	34.0	41.0
37	37.31869436941962	39.0	35.0	41.0	34.0	41.0
38	37.271361519263095	39.0	35.0	41.0	34.0	41.0
39	37.16095330876799	39.0	35.0	41.0	34.0	41.0
40	37.11902224599612	39.0	35.0	41.0	34.0	41.0
41	37.104974550070466	38.0	35.0	41.0	34.0	41.0
42	37.01828265289012	38.0	35.0	41.0	34.0	41.0
43	36.99865301761471	38.0	35.0	41.0	34.0	41.0
44	36.982771928997956	38.0	35.0	41.0	34.0	41.0
45	36.978742068116844	38.0	35.0	41.0	34.0	41.0
46	36.92812629484225	38.0	35.0	41.0	34.0	41.0
47	36.846746109064	38.0	35.0	41.0	34.0	41.0
48	36.85180699349929	37.0	35.0	41.0	34.0	41.0
49	36.79780852063364	37.0	35.0	41.0	34.0	41.0
50	36.70631903804394	37.0	35.0	41.0	34.0	41.0
51	36.65739807902574	37.0	35.0	41.0	34.0	41.0
52	36.4234776812502	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	3.0
13	0.0
14	2.0
15	2.0
16	7.0
17	29.0
18	40.0
19	94.0
20	194.0
21	315.0
22	518.0
23	936.0
24	1648.0
25	2693.0
26	3684.0
27	4163.0
28	4297.0
29	4794.0
30	5635.0
31	7064.0
32	9209.0
33	13098.0
34	41014.0
35	179287.0
36	25650.0
37	37005.0
38	65451.0
39	312482.0
40	2297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.98804206686964	21.595786106957608	24.85792245982265	20.558249366350108
2	25.97195449638518	24.06428376428917	27.149455456040844	22.814306283284807
3	25.910287092943168	24.290859505025548	26.376187790408434	23.42266561162285
4	26.100555283787845	27.089450993815245	23.95452964400586	22.855464078391048
5	23.78698831645217	34.16970037956633	21.709004688108447	20.33430661587305
6	85.68540200911015	2.6418592791426985	8.399654662540724	3.2730840492064304
7	87.40626901122901	2.0764592655620118	5.811979551366175	4.705292171842802
8	78.10571594469612	3.913870731264542	11.554254150077673	6.426159173961667
9	52.96897367425476	18.440493727247155	14.234776812501993	14.355755785996093
10	46.27605101349338	18.354990833036545	23.350881982447653	12.018076171022418
11	42.36550616466167	17.187467520679366	23.431534631443725	17.01549168321524
12	19.176760950814355	38.9670086320507	25.795959884314723	16.060270532820223
13	12.758223590761252	41.15197481198371	29.282870458265027	16.806931138990013
14	11.49480400159088	23.300162275347034	48.039738751934905	17.16529497112718
15	12.949877565952942	17.613457628950698	50.08487929125446	19.351785513841907
16	13.461924882173687	21.165084331906435	26.837653977963257	38.535336807956625
17	18.790820010171657	22.32360004600804	34.206562243196835	24.679017700623465
18	24.706179073824888	26.398360339960615	27.61604904567961	21.279411540534884
19	27.545374043982022	21.535365909427906	26.03930361564994	24.87995643094013
20	18.579210740383004	29.91201655180824	28.142785675978676	23.36598703183008
21	19.455303604563664	24.44579019502143	26.487882008777557	29.61102419163735
22	16.52312250472206	30.518158625191067	23.4043732582423	29.554345611844575
23	16.77145505970652	22.70926382978134	39.44510423176966	21.074176878742485
24	17.82368111439234	21.902321604516548	36.70277558746863	23.571221693622483
25	13.888607882618523	33.47084933336844	28.738672945193617	23.901869838819422
26	15.64245655219626	30.746674464013257	27.628659683237416	25.982209300553066
27	16.871092954256646	26.12037199995011	38.09368733656406	18.914847709229186
28	13.382796595959329	23.31596021690297	34.60871686069957	28.692526326438134
29	16.455496228587897	27.5420481615492	27.913438366548277	28.08901724331463
30	26.224167247541274	25.760622383465932	24.803183978115694	23.212026390877107
31	27.332656146715763	24.841015890789105	22.572348336296603	25.25397962619853
32	24.347399506383617	25.51215125004677	23.890090671869824	26.25035857169979
33	16.43609524772974	26.981359814748345	27.98258900546415	28.599955932057764
34	19.050238839932206	21.830953710645456	30.508596713196685	28.61021073622565
35	29.51110914021782	21.159818351387795	25.020613542161797	24.308458966232593
36	20.964145601589774	26.864953929599384	29.50182438509284	22.669076083718004
37	21.5952317932188	28.441422202759654	26.476795734001467	23.48655027002008
38	20.48979161960774	27.424672227357323	26.975678098925606	25.109858054109335
39	26.971936481188667	22.36461926267958	26.421087203251602	24.242357052880145
40	16.653940547079944	20.43519171633549	31.918493707846174	30.992374028738396
41	22.014570136624478	23.542120222335242	24.044328469692203	30.398981171348076
42	21.36934894465593	21.040363740675403	28.869213830682096	28.72107348398657
43	21.13154835070876	26.214328178677494	27.623532281153473	25.030591189460278
44	16.47129417014383	31.464787912634613	24.17279067866017	27.891127238561392
45	22.49377436382105	27.22165482052014	25.82824865960009	24.456322156058718
46	24.116943569475605	22.31639396740358	27.34083227436313	26.225830188757687
47	21.661888020310055	19.4597381144741	33.487063010228475	25.39131085498737
48	19.682156502169445	18.123149111781522	31.213406632086727	30.981287753962306
49	20.846215353659094	24.423201910165144	30.82261544622949	23.90796728994627
50	19.25782933511453	26.397944604656516	27.63780585992769	26.706420200301267
51	20.730502360683637	20.525129120456533	30.667823334668302	28.076545184191527
52	22.883456922200683	18.30357823376242	33.71751894713648	25.095445896900415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	205.5
2	237.0
3	1153.0
4	2069.0
5	1434.0
6	799.0
7	943.5
8	1088.0
9	1211.5
10	1335.0
11	1326.0
12	1317.0
13	1230.5
14	1108.5
15	1073.0
16	1001.5
17	930.0
18	997.5
19	1065.0
20	1197.5
21	1330.0
22	1447.0
23	1564.0
24	1943.5
25	2323.0
26	2420.5
27	2518.0
28	3494.0
29	4470.0
30	4949.0
31	5428.0
32	6045.5
33	6663.0
34	8240.5
35	9818.0
36	12095.5
37	14373.0
38	14716.5
39	18222.5
40	21385.0
41	29623.5
42	37862.0
43	49942.0
44	62022.0
45	67520.0
46	73018.0
47	72125.5
48	71233.0
49	76259.5
50	81286.0
51	84466.0
52	87646.0
53	75468.5
54	63291.0
55	52475.0
56	41659.0
57	35683.5
58	29708.0
59	25765.5
60	21823.0
61	20005.5
62	18188.0
63	16320.0
64	11205.5
65	7959.0
66	6997.5
67	6036.0
68	4753.5
69	3471.0
70	2753.0
71	2035.0
72	1791.5
73	1548.0
74	1323.0
75	1098.0
76	893.5
77	689.0
78	454.5
79	220.0
80	167.5
81	115.0
82	78.0
83	41.0
84	43.5
85	46.0
86	24.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	721613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.63090969834316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.27136845347603	16.81585911359882
2	13.123071632673886	6.464663846985583
3	5.275799772347228	3.8984324333767195
4	2.621001705415431	2.582306253011636
5	1.4662783543336901	1.8057884869114167
6	0.9172843280539223	1.3556128471200926
7	0.6057882437787977	1.0444780870183419
8	0.4605486706983075	0.9074986175729595
9	0.3797953416932239	0.8419234288587464
>10	5.714794883959443	38.78340846628585
>50	0.9686263816529461	14.98619490388475
>100	0.1860574539845547	7.254377090916073
>500	0.005074294199578765	0.8467004311672592
>1k	0.004510483732958902	2.4127559932917766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	4656	0.6452211919685482	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT	3612	0.500545306140549	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	3121	0.43250329470228505	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	1391	0.19276260266929782	Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1208	0.1674027491189876	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1174	0.16269108233914856	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.15770225868990717	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1074	0.14883323886903368	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	846	0.11723735575717177	No Hit
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	812	0.11252568897733273	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	800	0.11086274776091895	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	741	0.10268662011355116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.514541728045365	0.0
2	0.0	0.0	0.0	1.9285960757358862	0.0
3	0.0	0.0	0.0	3.009507866404846	0.0
4	0.0	0.0	0.0	3.6493244994200493	0.0
5	0.0	0.0	0.0	6.931277568447354	0.0
6	0.0	0.0	0.0	7.44110762971288	0.0
7	0.0	0.0	0.0	9.440933020885156	0.0
8	0.0	0.0	0.0	11.715420869635109	0.0
9	0.0	0.0	0.0	13.497123804587778	0.0
10	0.0	0.0	0.0	14.638594371221139	0.0
11	0.0	0.0	0.0	15.786578124285455	0.0
12	0.0	0.0	0.0	16.819264619678414	0.0
13	0.0	0.0	0.0	17.20853144275394	0.0
14	0.0	0.0	0.0	17.51478978344348	0.0
15	0.0	0.0	0.0	18.582952358119933	0.0
16	0.0	0.0	0.0	19.724145767883893	0.0
17	0.0	0.0	0.0	21.449724436782596	0.0
18	0.0	0.0	0.0	22.098825824922777	0.0
19	0.0	0.0	0.0	23.010671925256336	0.0
20	0.0	0.0	0.0	24.033519351785515	0.0
21	0.0	0.0	0.0	24.830761086621223	0.0
22	1.3857843470114868E-4	0.0	0.0	25.784042138930424	0.0
23	1.3857843470114868E-4	0.0	0.0	26.423581615076227	0.0
24	1.3857843470114868E-4	0.0	0.0	26.9514268728529	0.0
25	1.3857843470114868E-4	0.0	0.0	27.501028944877657	0.0
26	1.3857843470114868E-4	0.0	0.0	27.985499152592872	0.0
27	1.3857843470114868E-4	0.0	0.0	28.52817230288257	0.0
28	1.3857843470114868E-4	0.0	0.0	29.014721187118305	0.0
29	1.3857843470114868E-4	0.0	0.0	29.739902135909414	0.0
30	1.3857843470114868E-4	0.0	0.0	30.398426857609273	0.0
31	1.3857843470114868E-4	0.0	0.0	30.9083954973095	0.0
32	1.3857843470114868E-4	0.0	0.0	31.41767124483622	0.0
33	1.3857843470114868E-4	0.0	0.0	31.882324736389172	0.0
34	1.3857843470114868E-4	0.0	0.0	32.62912392099366	0.0
35	1.3857843470114868E-4	0.0	0.0	33.142834178430824	0.0
36	1.3857843470114868E-4	0.0	0.0	33.62370134684381	0.0
37	1.3857843470114868E-4	0.0	0.0	34.05606606311139	0.0
38	1.3857843470114868E-4	0.0	0.0	34.502842936587896	0.0
39	1.3857843470114868E-4	0.0	0.0	35.02112628237019	0.0
40	1.3857843470114868E-4	0.0	0.0	35.53358933389504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	70	0.0	46.000004	4
CTCGAGG	145	0.0	46.000004	1
TGTCCAA	35	1.0188705E-7	46.000004	34
GTCATAG	20	6.3098845E-4	46.0	41
CGGGTCA	40	5.6061253E-9	46.0	5
TGTCAAC	20	6.3098845E-4	46.0	33
CGGGTAC	20	6.3098845E-4	46.0	5
ATCTCGG	30	1.8604333E-6	46.0	1
AAATCGT	20	6.3098845E-4	46.0	11
ACGCCAT	20	6.3098845E-4	46.0	42
CATGCGG	155	0.0	46.0	1
CCGGATA	20	6.3098845E-4	46.0	19
CACGCTC	25	3.415883E-5	46.0	18
GGTCATA	25	3.415883E-5	46.0	7
CGATTGC	30	1.8604333E-6	46.0	9
CGATTCC	20	6.3098845E-4	46.0	9
ATCACGG	20	6.3098845E-4	46.0	1
CTAACGA	25	3.415883E-5	46.0	19
CGCTCAT	20	6.3098845E-4	46.0	20
TGCGCAC	25	3.415883E-5	46.0	21
>>END_MODULE