FastQCFastQC Report
Thu 26 May 2016
SRR1544867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544867_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences491902
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT49221.000605811726726Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT37670.7658029444889429Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT27220.5533622550833296Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTTG18150.3689759342308021Illumina Single End Adapter 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT11490.23358311208330115Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC10730.21813288012652926No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC10190.20715508373619135No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA9550.19414436208838345No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC9220.18743570873873253No Hit
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT7200.14637061853783884Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC6970.1416948904456579No Hit
CTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTTGAAA6390.12990392395233197Illumina Single End Adapter 2 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT6280.127667706169115No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG6210.126244658488886No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC5720.11628332472728307No Hit
GTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTTGA5140.10449235823395717Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.10225614045074019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA850.046.0000043
CCATAGG750.046.0000041
CTCGAGG750.046.0000041
AGGGCGA1450.046.0000045
CTCACGG206.307917E-446.01
CAAACCG206.307917E-446.023
CGAACAC301.8592164E-646.011
GTACATG551.8189894E-1246.040
TTTACGG301.8592164E-646.01
CCGATTC206.307917E-446.011
CATGCGG900.046.01
CATGCGC206.307917E-446.039
AACCCGC301.8592164E-646.021
ATAATGG405.6006684E-946.01
ATTACGG453.092282E-1046.01
CTCCCGT206.307917E-446.019
TTCACGG405.6006684E-946.01
CACGAGG453.092282E-1046.01
GAATAGT253.414286E-546.016
CACCTGG253.414286E-546.01
Produced by FastQC (version 0.11.5)