##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544867_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	491902
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.889593862192065	34.0	33.0	34.0	31.0	34.0
2	33.22327008225216	34.0	33.0	34.0	31.0	34.0
3	33.31234066948294	34.0	34.0	34.0	31.0	34.0
4	36.568265630145845	37.0	37.0	37.0	35.0	37.0
5	36.557182121642114	37.0	37.0	37.0	35.0	37.0
6	36.506759476481086	37.0	37.0	37.0	35.0	37.0
7	36.37998625742526	37.0	37.0	37.0	35.0	37.0
8	36.43505413679961	37.0	37.0	37.0	35.0	37.0
9	38.25251981085663	39.0	39.0	39.0	37.0	39.0
10	38.08392118755362	39.0	39.0	39.0	35.0	39.0
11	37.309151009753975	39.0	35.0	39.0	35.0	39.0
12	37.235122036503206	39.0	35.0	39.0	35.0	39.0
13	37.21127379030783	39.0	35.0	39.0	35.0	39.0
14	38.432275127972645	40.0	35.0	41.0	35.0	41.0
15	38.50722095051454	40.0	35.0	41.0	35.0	41.0
16	38.53014421571776	41.0	35.0	41.0	35.0	41.0
17	38.63004826164561	41.0	36.0	41.0	35.0	41.0
18	38.60951571654517	40.0	36.0	41.0	35.0	41.0
19	38.49946534065729	40.0	35.0	41.0	35.0	41.0
20	38.42794093132372	40.0	35.0	41.0	35.0	41.0
21	38.381236506458606	40.0	35.0	41.0	35.0	41.0
22	38.36636362527495	40.0	35.0	41.0	35.0	41.0
23	38.351791617029406	40.0	35.0	41.0	35.0	41.0
24	38.306868034689835	40.0	35.0	41.0	35.0	41.0
25	38.2581428821188	40.0	35.0	41.0	35.0	41.0
26	38.220336571105626	40.0	35.0	41.0	35.0	41.0
27	38.21890945757488	40.0	35.0	41.0	35.0	41.0
28	38.2633288744506	40.0	35.0	41.0	35.0	41.0
29	38.30811625079792	40.0	36.0	41.0	35.0	41.0
30	38.22483340177515	40.0	35.0	41.0	35.0	41.0
31	38.15849701769865	40.0	35.0	41.0	35.0	41.0
32	38.11410809470179	40.0	35.0	41.0	35.0	41.0
33	38.00296603795065	40.0	35.0	41.0	35.0	41.0
34	37.94552573480083	40.0	35.0	41.0	35.0	41.0
35	37.896052465735046	40.0	35.0	41.0	35.0	41.0
36	37.76968176588019	40.0	35.0	41.0	35.0	41.0
37	37.76799037206598	40.0	35.0	41.0	35.0	41.0
38	37.70132058824725	40.0	35.0	41.0	35.0	41.0
39	37.64506141467203	39.0	35.0	41.0	35.0	41.0
40	37.61878382279397	39.0	35.0	41.0	35.0	41.0
41	37.62321559985526	39.0	35.0	41.0	35.0	41.0
42	37.575435757528936	39.0	35.0	41.0	35.0	41.0
43	37.51261023537209	39.0	35.0	41.0	35.0	41.0
44	37.41078914092645	39.0	35.0	41.0	35.0	41.0
45	37.43143959569183	39.0	35.0	41.0	35.0	41.0
46	37.37171225162736	39.0	35.0	41.0	35.0	41.0
47	37.29768531130185	39.0	35.0	41.0	35.0	41.0
48	37.26578261523637	39.0	35.0	41.0	35.0	41.0
49	37.248793458859694	39.0	35.0	41.0	35.0	41.0
50	37.16692755874138	38.0	35.0	41.0	34.0	41.0
51	37.12246341750999	38.0	35.0	41.0	34.0	41.0
52	36.75450597883318	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	6.0
15	8.0
16	10.0
17	26.0
18	35.0
19	77.0
20	114.0
21	193.0
22	314.0
23	510.0
24	899.0
25	1422.0
26	1954.0
27	2237.0
28	2263.0
29	2388.0
30	2887.0
31	3668.0
32	4803.0
33	7250.0
34	23353.0
35	115650.0
36	16035.0
37	24140.0
38	45811.0
39	234682.0
40	1165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.035039499737756	21.29794145988429	23.7012656992653	22.96575334111266
2	25.528662213205067	24.344483250728803	27.059658224605716	23.067196311460414
3	25.9535435920163	24.157250834515818	26.4713296550939	23.417875918373984
4	26.124919191221014	26.50731243215112	24.35363141438742	23.014136962240446
5	23.829746575537403	33.025074100125636	22.118430093799173	21.02674923053779
6	83.22836662587262	2.7237132599582843	9.689531654679184	4.358388459489898
7	84.95472675451614	2.1845814816772444	7.019487621518107	5.841204142288505
8	74.37944956515729	4.213440888632289	13.47707470187151	7.930034844338913
9	50.61678952311639	18.01273424381279	15.65860679566255	15.711869437408263
10	43.50582026501213	18.206065435798187	25.723416452870694	12.564697846318982
11	39.55726953742818	17.15687271041793	24.47967278035056	18.806184971803326
12	18.648429971823653	36.47291533679473	27.703282361120714	17.175372330260906
13	12.62710865172331	39.07912551687125	30.44427548576749	17.849490345637953
14	11.326646364519762	23.46219368898683	46.29926286130164	18.911897085191764
15	12.476468890144785	17.999723522164985	49.17585210062167	20.34795548706856
16	13.030644315331102	22.417879984224502	27.170859236189322	37.38061646425507
17	17.65859866396152	22.627474578269656	34.21108269533362	25.502844062435198
18	23.38900837971791	26.70979178779513	28.36499953242719	21.536200300059768
19	26.062508385816685	21.36950042894723	26.2074559566743	26.360535228561787
20	17.98224036495074	30.640452773113342	28.00069932628857	23.376607535647345
21	19.01476310321975	24.51118312184134	26.421726278811636	30.05232749612728
22	16.122317046891453	30.697984557899744	23.33554244544645	29.844155949762353
23	15.964155461860289	22.894804249626958	38.94474915735248	22.196291131160272
24	18.460994263084924	21.860045293574736	35.35318010498026	24.32578033836008
25	13.881423535582291	32.542864229053755	28.65001565352448	24.92569658183947
26	15.81717496574521	29.026919996259416	27.071042606047545	28.084862431947826
27	17.812694398477745	25.128785815060724	36.90531853905859	20.153201247402937
28	13.492321641302535	23.090981536972812	33.16087350732463	30.255823314400022
29	16.863115010713518	26.281251143520457	26.722395924391446	30.133237921374583
30	25.47377323125338	25.31052933307854	24.617911697858517	24.597785737809563
31	27.27982402998971	23.87487751625324	21.6423189984997	27.20297945525735
32	24.036901659273596	24.10154868246114	24.13285573142618	27.72869392683909
33	16.353257356140045	26.137726620343077	28.18569552471834	29.323320498798537
34	20.114372374985262	20.094449707462054	30.446511703550705	29.344666214001975
35	28.863472805558832	20.74579895995544	24.806770454277476	25.583957780208255
36	21.413818199560076	25.925285930937463	28.903724725656737	23.757171143845728
37	22.359331736809366	27.448353533833973	27.06921297331582	23.123101756040835
38	21.04179287744307	26.384930331651425	26.34732121438823	26.225955576517272
39	27.712023939727832	21.28757354107119	26.563217876731542	24.437184642469433
40	17.151383812222758	19.456924346719468	32.109851149212645	31.281840691845126
41	22.265817174965747	23.237148862984903	23.791730873222715	30.705303088826636
42	21.087533695736145	19.948282381449964	29.48249854645844	29.481685376355454
43	22.313794211042037	24.632345467186553	27.552439307016442	25.50142101475497
44	16.724469508154062	29.068188378986058	25.553260608820455	28.654081504039425
45	22.296920931405037	25.579078759590324	26.100727380657123	26.023272928347517
46	24.821000931079766	20.46118942390964	27.802489113685247	26.915320531325342
47	20.78015539680668	18.32865082882363	34.59591544657269	26.295278327797
48	19.48579188537554	16.963541518432535	31.244434867107675	32.30623172908425
49	21.1037970977959	22.188566015181884	31.847807083524764	24.859829803497444
50	19.610816788709943	24.302401697899175	28.77280433907567	27.313977174315205
51	20.33677439815248	18.942797549105308	31.140958971502453	29.579469081239758
52	22.360144906912353	17.558578741294	35.23506714752126	24.846209204272395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	125.0
1	117.5
2	110.0
3	561.5
4	1013.0
5	755.0
6	497.0
7	557.0
8	617.0
9	670.0
10	723.0
11	787.0
12	851.0
13	776.0
14	670.0
15	639.0
16	580.0
17	521.0
18	565.5
19	610.0
20	727.5
21	845.0
22	878.5
23	912.0
24	1123.0
25	1334.0
26	1374.5
27	1415.0
28	1974.5
29	2534.0
30	2816.0
31	3098.0
32	3528.5
33	3959.0
34	4871.5
35	5784.0
36	7123.5
37	8463.0
38	9015.5
39	11387.0
40	13206.0
41	18212.5
42	23219.0
43	30832.5
44	38446.0
45	41832.0
46	45218.0
47	46422.5
48	47627.0
49	55007.5
50	62388.0
51	65073.0
52	67758.0
53	57400.0
54	47042.0
55	38370.0
56	29698.0
57	25175.0
58	20652.0
59	17606.0
60	14560.0
61	13196.0
62	11832.0
63	10966.0
64	7775.0
65	5450.0
66	4771.5
67	4093.0
68	3198.0
69	2303.0
70	1860.5
71	1418.0
72	1257.5
73	1097.0
74	901.5
75	706.0
76	593.5
77	481.0
78	318.5
79	156.0
80	112.0
81	68.0
82	49.0
83	30.0
84	30.5
85	31.0
86	16.5
87	2.0
88	1.5
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	491902.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.68031203393532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.7354876653722	20.00035546081522
2	12.441372866870907	7.13644911904788
3	4.555354068791768	3.9194692835401432
4	2.244741782121901	2.5751957898747273
5	1.2911589456259744	1.851542072297994
6	0.8178264180659192	1.407331011583572
7	0.5979255980780535	1.200408490816918
8	0.4965864175912338	1.1393802726664555
9	0.383515744641153	0.9899416103611793
>10	7.022762018249179	44.33418874151021
>50	0.29468456521576475	5.645291740151401
>100	0.10651249345148123	5.067932357635916
>500	0.007100832896765415	1.3789419326322196
>1k	0.004970583027735791	3.3535721170661295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	4922	1.000605811726726	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT	3767	0.7658029444889429	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	2722	0.5533622550833296	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTTG	1815	0.3689759342308021	Illumina Single End Adapter 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	1149	0.23358311208330115	Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1073	0.21813288012652926	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1019	0.20715508373619135	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	955	0.19414436208838345	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	922	0.18743570873873253	No Hit
ACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	720	0.14637061853783884	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	697	0.1416948904456579	No Hit
CTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTTGAAA	639	0.12990392395233197	Illumina Single End Adapter 2 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	628	0.127667706169115	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG	621	0.126244658488886	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	572	0.11628332472728307	No Hit
GTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTTGA	514	0.10449235823395717	Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	503	0.10225614045074019	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7812531764457148	0.0
2	0.0	0.0	0.0	2.750344580831141	0.0
3	0.0	0.0	0.0	4.120129619314416	0.0
4	0.0	0.0	0.0	4.968266036730894	0.0
5	0.0	0.0	0.0	9.056275436977284	0.0
6	0.0	0.0	0.0	9.704168716532967	0.0
7	0.0	0.0	0.0	12.228045423681953	0.0
8	0.0	0.0	0.0	14.972087936214937	0.0
9	0.0	0.0	0.0	17.124752491349902	0.0
10	0.0	0.0	0.0	18.52421823859224	0.0
11	0.0	0.0	0.0	19.914129237124467	0.0
12	0.0	0.0	0.0	21.194262271753317	0.0
13	0.0	0.0	0.0	21.667730564218076	0.0
14	0.0	0.0	0.0	22.029184674996237	0.0
15	0.0	0.0	0.0	23.253208972518916	0.0
16	0.0	0.0	0.0	24.587621111522214	0.0
17	0.0	0.0	0.0	26.395908128041764	0.0
18	0.0	0.0	0.0	27.093404783879716	0.0
19	0.0	0.0	0.0	28.07937353375266	0.0
20	0.0	0.0	0.0	29.162312818406917	0.0
21	0.0	0.0	0.0	30.01410850128684	0.0
22	0.0	0.0	0.0	31.049274042390557	0.0
23	0.0	0.0	0.0	31.7337599765807	0.0
24	0.0	0.0	0.0	32.311517334753674	0.0
25	0.0	0.0	0.0	32.89130761818411	0.0
26	0.0	0.0	0.0	33.41946160007481	0.0
27	0.0	0.0	0.0	33.998438713402265	0.0
28	0.0	0.0	0.0	34.51134575586194	0.0
29	0.0	0.0	0.0	35.207419364019664	0.0
30	0.0	0.0	0.0	35.856532398729826	0.0
31	0.0	0.0	0.0	36.36557688320031	0.0
32	0.0	0.0	0.0	36.894544035194	0.0
33	0.0	0.0	0.0	37.37106171554497	0.0
34	0.0	0.0	0.0	38.1000687128737	0.0
35	0.0	0.0	0.0	38.62415684424946	0.0
36	0.0	0.0	0.0	39.11144902846502	0.0
37	0.0	0.0	0.0	39.54039625779119	0.0
38	0.0	0.0	0.0	39.99882090335067	0.0
39	0.0	0.0	0.0	40.50136002699725	0.0
40	0.0	0.0	0.0	40.9959707421397	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	85	0.0	46.000004	3
CCATAGG	75	0.0	46.000004	1
CTCGAGG	75	0.0	46.000004	1
AGGGCGA	145	0.0	46.000004	5
CTCACGG	20	6.307917E-4	46.0	1
CAAACCG	20	6.307917E-4	46.0	23
CGAACAC	30	1.8592164E-6	46.0	11
GTACATG	55	1.8189894E-12	46.0	40
TTTACGG	30	1.8592164E-6	46.0	1
CCGATTC	20	6.307917E-4	46.0	11
CATGCGG	90	0.0	46.0	1
CATGCGC	20	6.307917E-4	46.0	39
AACCCGC	30	1.8592164E-6	46.0	21
ATAATGG	40	5.6006684E-9	46.0	1
ATTACGG	45	3.092282E-10	46.0	1
CTCCCGT	20	6.307917E-4	46.0	19
TTCACGG	40	5.6006684E-9	46.0	1
CACGAGG	45	3.092282E-10	46.0	1
GAATAGT	25	3.414286E-5	46.0	16
CACCTGG	25	3.414286E-5	46.0	1
>>END_MODULE