FastQCFastQC Report
Thu 26 May 2016
SRR1544866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544866_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences905419
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT50720.5601826336756794Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT41430.4575782041242784Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT32960.3640303550069084Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19100.21095205645121207No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18270.20178502991432695No Hit
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT14000.15462454399565284Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13280.14667242459016214No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC13220.1460097479730379Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC13080.14446350253308138Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC11030.12182205144800363Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC10170.11232368660255639Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT10170.11232368660255639No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT9730.10746405807697873No Hit
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT9130.10083729190573645Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG9100.10050595359717435No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATG206.310741E-446.05
CGAGTAA206.310741E-446.026
GTTTAGC301.8609626E-646.021
CGTTGAA206.310741E-446.017
ATTACGG253.4165765E-546.01
GGCAACC405.6097633E-946.07
ACGATGG750.046.01
GCCGATG206.310741E-446.08
ATGAGTC206.310741E-446.020
GCTTAGT253.4165765E-546.024
ACTACGG551.8189894E-1246.01
ACTAAGG405.6097633E-946.01
TGGATTG253.4165765E-546.05
TACGTTA206.310741E-446.011
GTCGGAT301.8609626E-646.021
CGAAGTT206.310741E-446.026
CGAAGGA253.4165765E-546.02
CTAGCGA253.4165765E-546.027
AATGCGG1500.044.4666631
CACGAGG1300.044.230771
Produced by FastQC (version 0.11.5)