##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544866_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	905419
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.892366959385654	34.0	33.0	34.0	31.0	34.0
2	33.2067915517567	34.0	33.0	34.0	31.0	34.0
3	33.26022758523954	34.0	34.0	34.0	31.0	34.0
4	36.55182959491683	37.0	37.0	37.0	35.0	37.0
5	36.54893811594411	37.0	37.0	37.0	35.0	37.0
6	36.655536276574715	37.0	37.0	37.0	35.0	37.0
7	36.62864927729593	37.0	37.0	37.0	35.0	37.0
8	36.52258346688108	37.0	37.0	37.0	35.0	37.0
9	38.31794561413003	39.0	39.0	39.0	37.0	39.0
10	38.10103940827396	39.0	39.0	39.0	35.0	39.0
11	37.31463775334955	39.0	35.0	39.0	35.0	39.0
12	37.19929999260011	39.0	35.0	39.0	35.0	39.0
13	37.18406284825037	39.0	35.0	39.0	35.0	39.0
14	38.43483072478046	40.0	35.0	41.0	35.0	41.0
15	38.518528990445304	41.0	35.0	41.0	35.0	41.0
16	38.507345218070306	41.0	35.0	41.0	35.0	41.0
17	38.58568795220776	41.0	36.0	41.0	35.0	41.0
18	38.556524658749154	40.0	36.0	41.0	35.0	41.0
19	38.460637561173336	40.0	35.0	41.0	35.0	41.0
20	38.372434198973075	40.0	35.0	41.0	35.0	41.0
21	38.308060687924595	40.0	35.0	41.0	35.0	41.0
22	38.28962833781929	40.0	35.0	41.0	35.0	41.0
23	38.252583610461016	40.0	35.0	41.0	35.0	41.0
24	38.196987251206345	40.0	35.0	41.0	35.0	41.0
25	38.15334116028049	40.0	35.0	41.0	35.0	41.0
26	38.09536358304829	40.0	35.0	41.0	35.0	41.0
27	38.07137027166428	40.0	35.0	41.0	35.0	41.0
28	38.10030825507307	40.0	35.0	41.0	35.0	41.0
29	38.12597924275943	40.0	35.0	41.0	35.0	41.0
30	38.04875532764389	40.0	35.0	41.0	35.0	41.0
31	37.97305335982567	40.0	35.0	41.0	35.0	41.0
32	37.907710131994136	40.0	35.0	41.0	35.0	41.0
33	37.829005134639324	40.0	35.0	41.0	35.0	41.0
34	37.74277433983603	40.0	35.0	41.0	35.0	41.0
35	37.682207905952936	40.0	35.0	41.0	35.0	41.0
36	37.58167765421313	40.0	35.0	41.0	35.0	41.0
37	37.51893984994793	40.0	35.0	41.0	34.0	41.0
38	37.456164494007744	39.0	35.0	41.0	35.0	41.0
39	37.33965600456805	39.0	35.0	41.0	34.0	41.0
40	37.26878715821073	39.0	35.0	41.0	34.0	41.0
41	37.244324450889586	39.0	35.0	41.0	34.0	41.0
42	37.157557992487455	39.0	35.0	41.0	34.0	41.0
43	37.17401004396859	39.0	35.0	41.0	34.0	41.0
44	37.15727414600312	39.0	35.0	41.0	34.0	41.0
45	37.12517740405271	38.0	35.0	41.0	34.0	41.0
46	37.076563447420476	38.0	35.0	41.0	34.0	41.0
47	37.00637053121262	38.0	35.0	41.0	34.0	41.0
48	37.006760405955696	38.0	35.0	41.0	34.0	41.0
49	36.95343481857571	38.0	35.0	41.0	34.0	41.0
50	36.86343339382098	37.0	35.0	41.0	34.0	41.0
51	36.79039980384772	37.0	35.0	41.0	34.0	41.0
52	36.5380713238843	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	4.0
15	10.0
16	13.0
17	22.0
18	60.0
19	132.0
20	212.0
21	390.0
22	639.0
23	1100.0
24	1862.0
25	2993.0
26	4167.0
27	5040.0
28	5203.0
29	5618.0
30	6596.0
31	8104.0
32	10623.0
33	15291.0
34	48905.0
35	204066.0
36	34539.0
37	52507.0
38	87425.0
39	406079.0
40	3815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.236435285762724	21.698241366704256	24.14031514690988	20.92500820062314
2	27.20464227059516	23.801466503353698	26.990266384955476	22.00362484109567
3	27.060841444679202	24.3582253078409	25.879178590243857	22.70175465723604
4	26.161037044727358	27.011251144497738	23.279719113471224	23.54799269730368
5	24.211994667662154	34.95906315197715	20.87994619065869	19.948995989702006
6	86.10610115316776	3.0005997223384973	7.946817992553725	2.9464811319400193
7	87.25253170079267	2.6262978797661636	5.845359993549948	4.275810425891217
8	77.17907399778446	5.218026129339013	10.74220885578942	6.860691017087117
9	52.76661965344221	19.667027089115646	13.659532216575974	13.906821040866163
10	43.55508333710691	19.54619905259333	23.186171264353852	13.712546345945912
11	41.0108469117613	17.88310163581723	23.270220748625775	17.8358307037957
12	19.996598260032094	35.4541930310718	26.498008104535025	18.051200604361075
13	14.298021137175166	37.36678819419517	28.627408967560875	19.707781701068786
14	13.591276525012178	24.88748303271745	42.73203897863862	18.789201463631755
15	15.759775308448354	17.769783934288988	46.06552325497918	20.404917502283475
16	16.070791534085323	21.811006837718228	26.2428776069422	35.875324021254244
17	19.747321405890535	23.110957468310254	31.297222611851527	25.844498513947684
18	24.83579425658176	27.159911598939274	26.180806897138233	21.823487247340733
19	29.01408077365286	21.747831666885716	24.470880332752017	24.767207226709402
20	20.60316825690647	29.632358057429762	27.39328421426986	22.371189471393908
21	21.10834873136084	24.01164543708493	26.36922794860722	28.510777882947014
22	19.329724690999416	28.919870247918368	22.719867818104102	29.03053724297811
23	19.57988511396381	22.045483914077348	37.14125725216723	21.233373719791608
24	19.80232356511184	21.44046016264293	33.48836284637278	25.26885342587244
25	16.582598774710934	31.989498784540636	27.635271625623055	23.792630815125374
26	16.965515413305884	30.71870592510208	27.144338698436854	25.17143996315518
27	18.86618239732102	26.866677195861804	34.295723858235796	19.971416548581374
28	15.744644192357352	24.97617125330924	31.199367364722853	28.079817189610555
29	17.876253977440278	30.310165790644994	27.56458611979647	24.248994112118258
30	25.901157364711807	25.79369330663483	22.688942909304973	25.61620641934839
31	26.437373194068158	27.760296614053825	22.299178612333073	23.50315157954494
32	23.875575838368754	31.30683142279983	22.62764532222098	22.18994741661043
33	17.034212889281093	26.95580720086501	27.503951209329603	28.50602870052429
34	17.614938498087625	28.43059401227498	27.347228189379724	26.607239300257675
35	27.77785754440762	24.58088465119464	24.428579475358923	23.212678329038823
36	19.67475831631543	32.001316517546016	26.08151585067245	22.242409315466098
37	20.638069225408348	29.927138705947193	25.941138853944967	23.493653214699492
38	19.951425803964796	30.087948231702672	24.611036437273793	25.34958952705874
39	25.569487717841135	25.847591004827596	25.529174890299412	23.053746387031858
40	16.23270552086934	23.455659755317704	30.85632176925821	29.455312954554742
41	20.834442396282824	26.920795786260282	22.471474532785372	29.773287284671518
42	21.427979753020423	23.727357168338635	26.49116044615808	28.353502632482865
43	21.583377419736056	28.08025897402197	26.475918883964223	23.86044472227775
44	17.980073314123075	32.0076119454087	24.385505495245848	25.626809245222375
45	21.06814634992197	27.913264466506664	25.82384509271398	25.19474409085738
46	22.36500448963408	25.636859840582094	26.057880384661686	25.940255285122138
47	21.50827406979531	21.91107100690399	32.02086547775119	24.55978944554952
48	19.103089287942932	21.818075388300887	29.215976249670046	29.86285907408614
49	19.46667785853842	26.077539790969706	30.440160853704196	24.015621496787677
50	18.318259281062137	27.388866370155696	27.78128137359609	26.511592975186076
51	20.10582945575474	23.04590471372922	28.62265978513815	28.225606045377887
52	21.716575419778025	21.144906391405527	31.61287757380837	25.525640615008076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	179.0
1	329.0
2	479.0
3	1903.0
4	3327.0
5	2229.5
6	1132.0
7	1257.5
8	1383.0
9	1467.0
10	1551.0
11	1477.0
12	1403.0
13	1352.0
14	1308.0
15	1315.0
16	1287.0
17	1259.0
18	1213.0
19	1167.0
20	1250.0
21	1333.0
22	1692.0
23	2051.0
24	1919.5
25	1788.0
26	2284.0
27	2780.0
28	4434.0
29	6088.0
30	6147.5
31	6207.0
32	7540.5
33	8874.0
34	10902.5
35	12931.0
36	14336.0
37	15741.0
38	17249.5
39	22992.0
40	27226.0
41	40083.5
42	52941.0
43	72884.5
44	92828.0
45	107201.0
46	121574.0
47	117514.0
48	113454.0
49	99167.5
50	84881.0
51	73431.0
52	61981.0
53	55562.5
54	49144.0
55	44116.5
56	39089.0
57	35659.5
58	32230.0
59	31939.0
60	31648.0
61	31762.5
62	31877.0
63	29383.0
64	21523.5
65	16158.0
66	13578.0
67	10998.0
68	9343.0
69	7688.0
70	6577.0
71	5466.0
72	4612.5
73	3759.0
74	2818.5
75	1878.0
76	1684.0
77	1490.0
78	1046.5
79	603.0
80	458.0
81	313.0
82	259.0
83	205.0
84	126.0
85	47.0
86	24.5
87	2.0
88	1.5
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	905419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.36030917571568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.48434176163954	16.92660048184632
2	12.626069053195085	6.151498916195361
3	4.998839565315034	3.653198319926233
4	2.433438818702811	2.3711728793515525
5	1.3773789091358966	1.6776688039330216
6	0.8448401432130785	1.2348340255635903
7	0.5660470247844129	0.9652356372219607
8	0.43777000727947624	0.8531370180146677
9	0.327631103386568	0.7183075476669899
>10	5.621785765956725	37.860371828277714
>50	1.0880358570579067	16.596351379324638
>100	0.18111235162310338	7.062663018053246
>500	0.007262650691653268	1.192996482054366
>1k	0.004993072350511622	2.17512594295036
>5k	4.5391566822832927E-4	0.5608377196199597
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	5072	0.5601826336756794	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT	4143	0.4575782041242784	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	3296	0.3640303550069084	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1910	0.21095205645121207	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1827	0.20178502991432695	No Hit
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	1400	0.15462454399565284	Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1328	0.14667242459016214	No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1322	0.1460097479730379	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1308	0.14446350253308138	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1103	0.12182205144800363	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1017	0.11232368660255639	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT	1017	0.11232368660255639	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	973	0.10746405807697873	No Hit
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	913	0.10083729190573645	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG	910	0.10050595359717435	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46829147610112004	0.0
2	0.0	0.0	0.0	1.6764613952214389	0.0
3	0.0	0.0	0.0	2.6144801467607817	0.0
4	0.0	0.0	0.0	3.1704658285280076	0.0
5	0.0	0.0	0.0	6.013569408196647	0.0
6	0.0	0.0	0.0	6.467613337029596	0.0
7	0.0	0.0	0.0	8.082004022447066	0.0
8	0.0	0.0	0.0	9.987861973296342	0.0
9	0.0	0.0	0.0	11.290573756459716	0.0
10	0.0	0.0	0.0	12.32302392593926	0.0
11	0.0	0.0	0.0	13.32410740220826	0.0
12	0.0	0.0	0.0	14.228881876788536	0.0
13	2.2089220570807548E-4	0.0	0.0	14.6067179946522	0.0
14	2.2089220570807548E-4	0.0	0.0	14.894430092586967	0.0
15	2.2089220570807548E-4	0.0	0.0	15.842388993383175	0.0
16	2.2089220570807548E-4	0.0	0.0	16.848111205972042	0.0
17	2.2089220570807548E-4	0.0	0.0	18.288328387188695	0.0
18	2.2089220570807548E-4	0.0	0.0	18.93786191807329	0.0
19	2.2089220570807548E-4	0.0	0.0	19.628923183631002	0.0
20	2.2089220570807548E-4	0.0	0.0	20.530826059537077	0.0
21	2.2089220570807548E-4	0.0	0.0	21.270704502556274	0.0
22	2.2089220570807548E-4	0.0	0.0	22.160789645456966	0.0
23	2.2089220570807548E-4	0.0	0.0	22.81661860420424	0.0
24	2.2089220570807548E-4	0.0	0.0	23.361891013994626	0.0
25	2.2089220570807548E-4	0.0	0.0	23.89402033754538	0.0
26	2.2089220570807548E-4	0.0	0.0	24.370153486949135	0.0
27	2.2089220570807548E-4	0.0	0.0	24.866056488763764	0.0
28	2.2089220570807548E-4	0.0	0.0	25.363837074326913	0.0
29	2.2089220570807548E-4	0.0	0.0	26.021543616822708	0.0
30	2.2089220570807548E-4	0.0	0.0	26.638605993468218	0.0
31	2.2089220570807548E-4	0.0	0.0	27.17780386760163	0.0
32	2.2089220570807548E-4	0.0	0.0	27.69502296726709	0.0
33	2.2089220570807548E-4	0.0	0.0	28.17259191600795	0.0
34	2.2089220570807548E-4	0.0	0.0	28.895792997496187	0.0
35	2.2089220570807548E-4	0.0	0.0	29.384737894831012	0.0
36	2.2089220570807548E-4	0.0	0.0	29.930562535135667	0.0
37	2.2089220570807548E-4	0.0	0.0	30.408462822185086	0.0
38	2.2089220570807548E-4	0.0	0.0	30.858088906903877	0.0
39	2.2089220570807548E-4	0.0	0.0	31.39761811934585	0.0
40	2.2089220570807548E-4	0.0	0.0	31.944326328473338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTATG	20	6.310741E-4	46.0	5
CGAGTAA	20	6.310741E-4	46.0	26
GTTTAGC	30	1.8609626E-6	46.0	21
CGTTGAA	20	6.310741E-4	46.0	17
ATTACGG	25	3.4165765E-5	46.0	1
GGCAACC	40	5.6097633E-9	46.0	7
ACGATGG	75	0.0	46.0	1
GCCGATG	20	6.310741E-4	46.0	8
ATGAGTC	20	6.310741E-4	46.0	20
GCTTAGT	25	3.4165765E-5	46.0	24
ACTACGG	55	1.8189894E-12	46.0	1
ACTAAGG	40	5.6097633E-9	46.0	1
TGGATTG	25	3.4165765E-5	46.0	5
TACGTTA	20	6.310741E-4	46.0	11
GTCGGAT	30	1.8609626E-6	46.0	21
CGAAGTT	20	6.310741E-4	46.0	26
CGAAGGA	25	3.4165765E-5	46.0	2
CTAGCGA	25	3.4165765E-5	46.0	27
AATGCGG	150	0.0	44.466663	1
CACGAGG	130	0.0	44.23077	1
>>END_MODULE