FastQCFastQC Report
Thu 26 May 2016
SRR1544865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544865_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences677164
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT62910.9290216254851115Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT48910.7222770259494008Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT34880.5150894022718279Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA27440.405219415089993No Hit
TGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTTG25070.3702205078828762Illumina Single End Adapter 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC13550.20009923740777713Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT13150.19419224884961397Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC12630.18651316372400187No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC12230.1806061751658387Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC11270.1664294026262471Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC10110.14929913580757392Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT9150.13512236326798235Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8390.12389908500747235No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG8170.1206502413004826No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT7910.11681069873767655No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA7670.11326650560277865No Hit
GGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC6830.100861829630636Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC501.6370905E-1146.024
CGGGTAC206.3096086E-446.05
CTATGAT206.3096086E-446.027
GTACCGG253.4156597E-546.01
CGACGGT501.6370905E-1146.027
ATTACGG405.6061253E-946.01
GGGTACG253.4156597E-546.06
AAGACGG253.4156597E-546.01
ATCCGCG253.4156597E-546.09
AATGCGG551.8189894E-1246.01
TATCTAG206.3096086E-446.012
TTGACGG600.046.01
ACTACGG405.6061253E-946.01
GACGGTC551.8189894E-1246.028
CGGTCTA551.8189894E-1246.030
CAGTACG253.4156597E-546.032
GGTTCGC253.4156597E-546.08
CCACGTG253.4156597E-546.021
ATCGAGG301.8602623E-646.01
GTAGCGG900.045.9999961
Produced by FastQC (version 0.11.5)