##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544865_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	677164
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88904903391202	34.0	33.0	34.0	31.0	34.0
2	33.23625591437229	34.0	33.0	34.0	31.0	34.0
3	33.325875268029606	34.0	34.0	34.0	31.0	34.0
4	36.56584815495212	37.0	37.0	37.0	35.0	37.0
5	36.56908961492341	37.0	37.0	37.0	35.0	37.0
6	36.4976844604852	37.0	37.0	37.0	35.0	37.0
7	36.37342652592282	37.0	37.0	37.0	35.0	37.0
8	36.411513311398714	37.0	37.0	37.0	35.0	37.0
9	38.268353013450216	39.0	39.0	39.0	37.0	39.0
10	38.13256611396944	39.0	39.0	39.0	35.0	39.0
11	37.46253197157557	39.0	35.0	39.0	35.0	39.0
12	37.39977907862792	39.0	35.0	39.0	35.0	39.0
13	37.38729761180453	39.0	35.0	39.0	35.0	39.0
14	38.63937244153558	41.0	35.0	41.0	35.0	41.0
15	38.70916498809742	41.0	35.0	41.0	35.0	41.0
16	38.71516648847251	41.0	35.0	41.0	35.0	41.0
17	38.79220543324808	41.0	36.0	41.0	35.0	41.0
18	38.77705548434353	41.0	36.0	41.0	35.0	41.0
19	38.70086714592034	41.0	35.0	41.0	35.0	41.0
20	38.62415308551547	41.0	35.0	41.0	35.0	41.0
21	38.57174775977459	41.0	35.0	41.0	35.0	41.0
22	38.55214541824432	41.0	35.0	41.0	35.0	41.0
23	38.52383912907361	40.0	35.0	41.0	35.0	41.0
24	38.45740175201281	40.0	35.0	41.0	35.0	41.0
25	38.403690981800565	40.0	35.0	41.0	35.0	41.0
26	38.34965975745905	40.0	35.0	41.0	35.0	41.0
27	38.33715023244	40.0	35.0	41.0	35.0	41.0
28	38.40013645143569	40.0	35.0	41.0	35.0	41.0
29	38.435575429290395	40.0	36.0	41.0	35.0	41.0
30	38.38392915157923	40.0	35.0	41.0	35.0	41.0
31	38.34862898795565	40.0	35.0	41.0	35.0	41.0
32	38.31563402661689	40.0	35.0	41.0	35.0	41.0
33	38.245318711567656	40.0	35.0	41.0	35.0	41.0
34	38.19703646384037	40.0	35.0	41.0	35.0	41.0
35	38.11601916227088	40.0	35.0	41.0	35.0	41.0
36	38.07251419154001	40.0	35.0	41.0	35.0	41.0
37	38.02396465258047	40.0	35.0	41.0	35.0	41.0
38	37.96099615455045	40.0	35.0	41.0	35.0	41.0
39	37.91294280262979	40.0	35.0	41.0	35.0	41.0
40	37.859898045377484	40.0	35.0	41.0	35.0	41.0
41	37.829456379842995	40.0	35.0	41.0	35.0	41.0
42	37.775067782693704	40.0	35.0	41.0	35.0	41.0
43	37.70546278301859	40.0	35.0	41.0	35.0	41.0
44	37.5883862697958	39.0	35.0	41.0	35.0	41.0
45	37.57213762101943	39.0	35.0	41.0	35.0	41.0
46	37.52327944190772	39.0	35.0	41.0	35.0	41.0
47	37.46810373853306	39.0	35.0	41.0	35.0	41.0
48	37.434971439710324	39.0	35.0	41.0	35.0	41.0
49	37.40327601585436	39.0	35.0	41.0	35.0	41.0
50	37.31622029523129	39.0	35.0	41.0	35.0	41.0
51	37.24667584218889	38.0	35.0	41.0	35.0	41.0
52	36.84743725301404	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	3.0
14	2.0
15	10.0
16	10.0
17	24.0
18	57.0
19	89.0
20	171.0
21	272.0
22	390.0
23	625.0
24	1015.0
25	1706.0
26	2332.0
27	2754.0
28	2822.0
29	3171.0
30	3703.0
31	4664.0
32	6322.0
33	9582.0
34	31152.0
35	142063.0
36	24267.0
37	38100.0
38	65149.0
39	331794.0
40	4910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.36660543088528	21.427896344164783	23.028247219285134	23.1772510056648
2	26.985486529112595	23.793792936423085	26.78996520783739	22.430755326626933
3	27.292502259423124	23.970559569026115	26.00787992273659	22.72905824881417
4	26.245636212202655	26.27546650442138	23.707255554045993	23.77164172932997
5	24.107454028861547	33.68637434949289	21.244189000005907	20.961982621639663
6	83.48509371437348	3.0037036818259684	9.389010638486393	4.122191965314164
7	84.85241389087429	2.667300683438576	6.99874771842567	5.48153770726146
8	72.98305875681518	5.370486322367993	12.938815412514545	8.707639508302274
9	50.26684820811502	18.82483416129623	15.15925241152808	15.74906521906067
10	40.744191953500184	18.995693805341098	25.999462464041205	14.26065177711751
11	37.93202237567266	17.710776119226658	24.36750920013468	19.989692304966006
12	19.317033982905173	32.87002853075474	28.748722613724297	19.064214872615793
13	14.022747812937485	35.27963683834344	30.08975669114129	20.60785865757778
14	13.405615183323391	24.875214866708802	41.08354844616666	20.635621503801147
15	15.17889314848397	18.061355890153642	45.2048543631971	21.55489659816529
16	15.38696682044527	23.247248820079037	26.45430058301977	34.911483776455924
17	18.537606842655546	23.57331458848964	31.386045330230196	26.503033238624617
18	23.366422314239976	27.641309933782658	27.04440873998027	21.947859011997096
19	27.212314889746057	21.662256115209903	25.00206744599536	26.123361549048678
20	19.421587680384665	30.471495826712587	27.633778523371	22.473137969531752
21	20.380439598088497	23.969525846028436	26.624126504066965	29.025908051816103
22	18.676125724344473	28.974517251360083	22.784140917119046	29.5652161071764
23	18.524906817255495	22.281751540247267	36.96593439698507	22.227407245512165
24	20.243840487680977	21.914927550785336	32.22321328363587	25.61801867789782
25	16.40798979272377	31.352523170162623	27.483741013993658	24.755746023119954
26	16.611928572694353	29.91225168496849	26.290676999958652	27.18514274237851
27	19.937563130940212	26.276352552705106	32.683515367030736	21.102568949323945
28	15.798979272377151	24.899876543939133	29.73282690751428	29.568317276169438
29	17.950747529402037	30.694484644783245	26.13945809286967	25.215309732945045
30	24.934580101718343	25.817231867021874	22.067623205013852	27.180564826245934
31	25.441104370580835	27.80995445711822	21.27180417151532	25.47713700078563
32	22.885150421463635	31.885628887536843	21.970748592659977	23.258472098339546
33	16.44860033906114	26.651741675576375	27.62358896810817	29.276069017254315
34	17.662929511905535	28.56678736613287	26.57952874045283	27.190754381508764
35	27.113963530252644	24.62239575641942	24.366623151850956	23.897017561476982
36	19.535297210129304	32.630795494149126	25.043711715330407	22.790195580391163
37	20.73855077942714	29.74523158348642	26.61364159937622	22.902576037710215
38	19.952330602335625	29.89585979171958	23.718183482878594	26.4336261230662
39	26.082012629141538	25.7747015494031	25.556290647465012	22.58699517399035
40	16.463958509312366	23.229675529118502	30.907431582305023	29.39893437926411
41	20.6901134732502	27.105841420985165	22.02302544139972	30.18101966436491
42	21.00244549326308	22.789604881535343	27.02801684673137	29.179932778470207
43	22.441387906031625	26.91401196755882	26.28993862638888	24.354661500020676
44	17.97836270091145	30.179690591939323	25.382329834427107	26.45961687272212
45	20.592057463184695	26.33291196814952	26.312680532337808	26.76235003632798
46	22.86137479251703	24.099036570166163	26.324789858882042	26.71479877843477
47	20.424003638704953	21.212291261791826	33.08341849241838	25.28028660708484
48	18.827935330289268	20.728656573592215	28.890490339120213	31.552917756998305
49	19.58831243243882	24.082940026345167	31.308959129546167	25.019788411669847
50	18.677011772628198	25.098351359493414	29.044072041632457	27.180564826245934
51	19.966507374875214	21.459203383523047	28.813994837292	29.76029440430974
52	21.09666196076578	20.136480970636363	33.66008825040906	25.1067688181888
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	185.0
2	242.0
3	948.0
4	1654.0
5	1177.5
6	701.0
7	729.0
8	757.0
9	838.0
10	919.0
11	878.5
12	838.0
13	808.5
14	807.5
15	836.0
16	816.0
17	796.0
18	769.5
19	743.0
20	779.0
21	815.0
22	1060.0
23	1305.0
24	1242.0
25	1179.0
26	1484.0
27	1789.0
28	2678.0
29	3567.0
30	3783.0
31	3999.0
32	4759.5
33	5520.0
34	6885.0
35	8250.0
36	9375.5
37	10501.0
38	11525.5
39	15362.5
40	18175.0
41	27682.0
42	37189.0
43	52171.0
44	67153.0
45	82414.0
46	97675.0
47	95941.5
48	94208.0
49	81833.5
50	69459.0
51	58855.5
52	48252.0
53	42752.5
54	37253.0
55	32868.0
56	28483.0
57	25704.5
58	22926.0
59	22213.5
60	21501.0
61	21546.5
62	21592.0
63	21252.5
64	16201.0
65	11489.0
66	9649.0
67	7809.0
68	6612.0
69	5415.0
70	4651.5
71	3888.0
72	3285.0
73	2682.0
74	2008.0
75	1334.0
76	1204.5
77	1075.0
78	763.0
79	451.0
80	337.0
81	223.0
82	172.5
83	122.0
84	72.5
85	23.0
86	12.0
87	1.0
88	1.5
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	677164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.431713270560643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4798912950173	19.059524560746365
2	12.524695894432453	6.87147733154078
3	4.776827449327116	3.9310968279865492
4	2.293967561621886	2.5170984160951493
5	1.2836072800373939	1.7605773428995015
6	0.7734342900376312	1.2729976606759164
7	0.5733468009506179	1.100951953378939
8	0.45625933895547505	1.0012780290593704
9	0.4004175293303072	0.9885724967785763
>10	6.979727792442452	44.43125537255334
>50	0.3347303727185956	6.245247677015178
>100	0.10851742728456633	5.249420282951606
>500	0.008638203166930652	1.5399484300220285
>1k	0.005398876979331658	3.0988537170399044
>5k	5.398876979331658E-4	0.9316999012568361
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	6291	0.9290216254851115	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT	4891	0.7222770259494008	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	3488	0.5150894022718279	Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2744	0.405219415089993	No Hit
TGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTTG	2507	0.3702205078828762	Illumina Single End Adapter 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1355	0.20009923740777713	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	1315	0.19419224884961397	Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1263	0.18651316372400187	No Hit
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1223	0.1806061751658387	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1127	0.1664294026262471	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	1011	0.14929913580757392	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	915	0.13512236326798235	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	839	0.12389908500747235	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG	817	0.1206502413004826	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT	791	0.11681069873767655	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	767	0.11326650560277865	No Hit
GGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	683	0.100861829630636	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7354200754913137	0.0
2	0.0	0.0	0.0	2.547536490421818	0.0
3	0.0	0.0	0.0	3.8274332362618213	0.0
4	0.0	0.0	0.0	4.6423023078604295	0.0
5	0.0	0.0	0.0	8.503700728331689	0.0
6	0.0	0.0	0.0	9.107690308403873	0.0
7	0.0	0.0	0.0	11.277327205817203	0.0
8	0.0	0.0	0.0	13.804779935141266	0.0
9	0.0	0.0	0.0	15.59444979355075	0.0
10	0.0	0.0	0.0	17.00311888995871	0.0
11	0.0	0.0	0.0	18.316685470580243	0.0
12	0.0	0.0	0.0	19.498673881068694	0.0
13	0.0	0.0	0.0	19.976696930138047	0.0
14	0.0	0.0	0.0	20.331411593055744	0.0
15	0.0	0.0	0.0	21.506163942560445	0.0
16	0.0	0.0	0.0	22.713700078562947	0.0
17	0.0	0.0	0.0	24.336202160776416	0.0
18	0.0	0.0	0.0	25.102338576770176	0.0
19	0.0	0.0	0.0	25.877926174457	0.0
20	0.0	0.0	0.0	26.91799918483558	0.0
21	0.0	0.0	0.0	27.753690391101713	0.0
22	0.0	0.0	0.0	28.758026120703406	0.0
23	0.0	0.0	0.0	29.490788051343543	0.0
24	0.0	0.0	0.0	30.1045241625367	0.0
25	0.0	0.0	0.0	30.67632065496689	0.0
26	0.0	0.0	0.0	31.21267521604811	0.0
27	0.0	0.0	0.0	31.74962047598514	0.0
28	0.0	0.0	0.0	32.29380179690592	0.0
29	0.0	0.0	0.0	32.973105481094684	0.0
30	0.0	0.0	0.0	33.61681955922052	0.0
31	0.0	0.0	0.0	34.17251950782971	0.0
32	0.0	0.0	0.0	34.731911324287765	0.0
33	0.0	0.0	0.0	35.2055041319385	0.0
34	0.0	0.0	0.0	35.947126545415884	0.0
35	0.0	0.0	0.0	36.45128801885511	0.0
36	0.0	0.0	0.0	36.99458329149216	0.0
37	0.0	0.0	0.0	37.487964510812745	0.0
38	0.0	0.0	0.0	37.985923646265896	0.0
39	0.0	0.0	0.0	38.53423985917739	0.0
40	0.0	0.0	0.0	39.07000372140279	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	50	1.6370905E-11	46.0	24
CGGGTAC	20	6.3096086E-4	46.0	5
CTATGAT	20	6.3096086E-4	46.0	27
GTACCGG	25	3.4156597E-5	46.0	1
CGACGGT	50	1.6370905E-11	46.0	27
ATTACGG	40	5.6061253E-9	46.0	1
GGGTACG	25	3.4156597E-5	46.0	6
AAGACGG	25	3.4156597E-5	46.0	1
ATCCGCG	25	3.4156597E-5	46.0	9
AATGCGG	55	1.8189894E-12	46.0	1
TATCTAG	20	6.3096086E-4	46.0	12
TTGACGG	60	0.0	46.0	1
ACTACGG	40	5.6061253E-9	46.0	1
GACGGTC	55	1.8189894E-12	46.0	28
CGGTCTA	55	1.8189894E-12	46.0	30
CAGTACG	25	3.4156597E-5	46.0	32
GGTTCGC	25	3.4156597E-5	46.0	8
CCACGTG	25	3.4156597E-5	46.0	21
ATCGAGG	30	1.8602623E-6	46.0	1
GTAGCGG	90	0.0	45.999996	1
>>END_MODULE