FastQCFastQC Report
Thu 26 May 2016
SRR1544864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544864_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences607030
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT35830.590250893695534Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT26310.43342174192379285Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT19420.3199182906940349Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9970.16424229445002717No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT8790.14480338698252146Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC8300.13673129828838773Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC8240.13574287926461623Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC8170.13458972373688285Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT7080.11663344480503436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAA551.8189894E-1246.00000418
TTAGGCA206.309089E-446.012
ACGCCCA206.309089E-446.035
CGTATCT206.309089E-446.034
GGCACCG1800.046.07
CATGACG253.415237E-546.029
ATTACGT206.309089E-446.021
GCTATCC206.309089E-446.042
TTCGTAG253.415237E-546.030
TACGGGT405.6043064E-946.03
ACGGGTC301.8599403E-646.04
GTTACGG501.6370905E-1146.01
AATGCGG600.046.01
CCACTAC206.309089E-446.015
ACCTAGG405.6043064E-946.01
TACGCGG600.046.01
AGGCCCG253.415237E-546.032
CGCTATA206.309089E-446.019
TGCGACA253.415237E-546.020
AAACCGG253.415237E-546.01
Produced by FastQC (version 0.11.5)