##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544864_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	607030
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89307612473848	34.0	33.0	34.0	31.0	34.0
2	33.20917088117556	34.0	33.0	34.0	31.0	34.0
3	33.26040393390772	34.0	34.0	34.0	31.0	34.0
4	36.54916066751231	37.0	37.0	37.0	35.0	37.0
5	36.54277877534883	37.0	37.0	37.0	35.0	37.0
6	36.66149448956394	37.0	37.0	37.0	35.0	37.0
7	36.64440801937301	37.0	37.0	37.0	35.0	37.0
8	36.551028779467245	37.0	37.0	37.0	35.0	37.0
9	38.33446617135891	39.0	39.0	39.0	37.0	39.0
10	38.062687181852624	39.0	39.0	39.0	35.0	39.0
11	37.15278322323444	39.0	35.0	39.0	35.0	39.0
12	37.047999275159384	39.0	35.0	39.0	35.0	39.0
13	37.042370228819	39.0	35.0	39.0	35.0	39.0
14	38.23975091840601	40.0	35.0	41.0	35.0	41.0
15	38.314381496795875	40.0	35.0	41.0	35.0	41.0
16	38.31717213317299	40.0	35.0	41.0	35.0	41.0
17	38.39727031612935	40.0	36.0	41.0	35.0	41.0
18	38.369690130636045	40.0	36.0	41.0	35.0	41.0
19	38.253427342964926	40.0	35.0	41.0	35.0	41.0
20	38.172797061100766	40.0	35.0	41.0	35.0	41.0
21	38.119521275719485	40.0	35.0	41.0	35.0	41.0
22	38.09835263496038	40.0	35.0	41.0	35.0	41.0
23	38.06305454425646	40.0	35.0	41.0	35.0	41.0
24	38.0069271699916	40.0	35.0	41.0	35.0	41.0
25	37.991857074609165	40.0	35.0	41.0	35.0	41.0
26	37.92993921223004	40.0	35.0	41.0	35.0	41.0
27	37.931196151755266	40.0	35.0	41.0	35.0	41.0
28	37.97902080622045	40.0	35.0	41.0	35.0	41.0
29	37.966619442202195	40.0	35.0	41.0	35.0	41.0
30	37.858545706143026	40.0	35.0	41.0	35.0	41.0
31	37.79346325552279	40.0	35.0	41.0	35.0	41.0
32	37.695173220433915	40.0	35.0	41.0	35.0	41.0
33	37.61536167899445	40.0	35.0	41.0	35.0	41.0
34	37.51867947218424	39.0	35.0	41.0	35.0	41.0
35	37.47392056405779	39.0	35.0	41.0	35.0	41.0
36	37.37218918340115	39.0	35.0	41.0	34.0	41.0
37	37.297830420242825	39.0	35.0	41.0	34.0	41.0
38	37.282885524603394	39.0	35.0	41.0	34.0	41.0
39	37.15547501770917	39.0	35.0	41.0	34.0	41.0
40	37.09320791394165	38.0	35.0	41.0	34.0	41.0
41	37.06552723918093	38.0	35.0	41.0	34.0	41.0
42	36.97385796418629	38.0	35.0	41.0	34.0	41.0
43	36.973291270612656	38.0	35.0	41.0	34.0	41.0
44	36.934322850600466	38.0	35.0	41.0	34.0	41.0
45	36.9300166383869	38.0	35.0	41.0	34.0	41.0
46	36.89971994794327	37.0	35.0	41.0	34.0	41.0
47	36.818452135808776	37.0	35.0	41.0	34.0	41.0
48	36.82388514570944	37.0	35.0	41.0	34.0	41.0
49	36.7581371596132	37.0	35.0	41.0	34.0	41.0
50	36.67003772465941	37.0	35.0	41.0	34.0	41.0
51	36.5832347659918	36.0	35.0	41.0	34.0	41.0
52	36.351873877732565	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	7.0
15	3.0
16	13.0
17	20.0
18	29.0
19	80.0
20	161.0
21	259.0
22	408.0
23	778.0
24	1361.0
25	2193.0
26	2998.0
27	3431.0
28	3451.0
29	3705.0
30	4364.0
31	5602.0
32	7401.0
33	10441.0
34	34422.0
35	154917.0
36	22521.0
37	33713.0
38	55782.0
39	256359.0
40	2608.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.894469795561996	21.473073818427427	25.089040080391413	21.54341630561916
2	25.723934566660628	24.544091725285405	27.334398629392286	22.39757507866168
3	26.32950595522462	25.108973197370805	26.273495543877566	22.28802530352701
4	26.718943050590582	26.589130685468593	23.648419353244485	23.04350691069634
5	24.7867485956213	34.18578982916824	20.630940810174124	20.396520765036325
6	86.66672157883465	2.744839629013393	7.682486862263809	2.9059519298881438
7	88.12694594995305	2.3007100143320756	5.426914650017298	4.145429385697576
8	78.99840205591157	4.167998286740359	10.847239839876119	5.9863598174719534
9	55.49643345468922	17.56107605884388	13.135100406899165	13.80739007956773
10	47.516267729766234	19.097738167800603	21.2556216331977	12.130372469235457
11	42.268586396059504	18.030904568143256	23.12439253414164	16.5761165016556
12	20.048597268668765	38.59446814819696	25.373704759237597	15.983229823896677
13	13.42569559988798	41.58707147916907	28.573217139185875	16.41401578175708
14	11.692338105200731	23.240861242442712	48.37816911849497	16.68863153386159
15	13.39390145462333	16.826351251173747	49.60990395861819	20.169843335584734
16	14.43240037559923	19.780900449730655	26.633939014546232	39.15276016012388
17	19.964911124656112	22.45506811854439	33.25700541983098	24.323015336968517
18	26.089155395944186	25.23301978485413	26.565573365402038	22.112251453799647
19	28.60995337956938	21.734675386718944	24.467489250943117	25.187881982768562
20	20.300808856234454	28.89626542345518	27.945900532098904	22.857025188211455
21	20.494538984893662	24.55463486153897	25.866102169579758	29.084723983987608
22	18.10783651549347	29.776123091115757	22.905128247368335	29.210912146022437
23	19.105480783486815	21.527601601238818	38.67370640660263	20.69321120867173
24	18.624944401429914	21.720178574370294	35.41324152018846	24.241635504011334
25	15.906627349554386	32.603001499102184	27.847717575737608	23.642653575605816
26	17.403917434064216	30.352865591486417	28.806154555788016	23.437062418661352
27	18.81093191440291	25.78702864767804	37.74558094328122	17.65645849463783
28	15.415218358236002	23.604269970182692	33.59240894189743	27.388102729683872
29	17.928273726174986	26.0194718547683	31.37340823353047	24.67884618552625
30	29.705780603924026	24.600596346144343	21.593825675831507	24.099797374100127
31	27.750028828888198	25.635965273544965	23.354694166680396	23.25931173088645
32	25.703013030657463	26.915638436321103	23.401808806813502	23.97953972620793
33	18.988682602177818	26.10414641780472	26.18387888572229	28.72329209429518
34	18.586231322998863	25.92936098710113	28.02365616196893	27.460751527931077
35	30.112515032205987	20.449730655816023	24.73057344777029	24.7071808642077
36	20.996491112465613	29.476632126913003	25.593957465034677	23.93291929558671
37	21.65082450620233	28.432696901306358	24.34031266988452	25.57616592260679
38	20.234420045137803	29.09955026934418	24.051364841935325	26.61466484358269
39	25.67550203449582	24.765168113602293	24.67192725235985	24.887402599542032
40	17.594847042156072	21.66268553448759	29.52358203054215	31.218885392814194
41	22.74632225754905	25.856547452349965	23.33360789417327	28.063522395927716
42	23.901454623329986	22.574502083916776	26.027543943462433	27.496499349290808
43	22.34749518145726	26.93837207386785	26.300018121015434	24.414114623659458
44	17.635701695138625	31.097639325898225	23.29835428232542	27.96830469663773
45	22.67466187832562	26.7482661482958	25.030723357988897	25.546348615389686
46	24.232410259789468	24.44805034347561	25.89822578785233	25.42131360888259
47	23.535574848030574	20.577566182890468	30.47345271238654	25.413406256692422
48	20.182363309885837	19.601667133420094	29.31041299441543	30.905556562278637
49	20.398168130075945	25.914369965240596	29.386356522741874	24.301105381941586
50	18.989671021201588	27.946559478114757	25.80959754872082	27.254171951962835
51	20.71825115727394	22.15376505279805	28.949804787242805	28.178179002685205
52	23.27314959721925	20.398332866579906	31.644564519051777	24.683953017149072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186.0
1	243.0
2	300.0
3	1027.0
4	1754.0
5	1263.5
6	773.0
7	866.0
8	959.0
9	1025.5
10	1092.0
11	1081.5
12	1071.0
13	1043.0
14	988.5
15	962.0
16	865.0
17	768.0
18	823.0
19	878.0
20	989.5
21	1101.0
22	1037.5
23	974.0
24	1064.5
25	1155.0
26	1606.5
27	2058.0
28	2328.0
29	2598.0
30	3071.5
31	3545.0
32	4258.0
33	4971.0
34	6528.0
35	8085.0
36	8655.0
37	9225.0
38	10424.5
39	13414.0
40	15204.0
41	23278.0
42	31352.0
43	41791.5
44	52231.0
45	56758.5
46	61286.0
47	60829.5
48	60373.0
49	63650.0
50	66927.0
51	67458.0
52	67989.0
53	60490.0
54	52991.0
55	44182.0
56	35373.0
57	31125.5
58	26878.0
59	24514.0
60	22150.0
61	20305.0
62	18460.0
63	16564.0
64	12131.0
65	9594.0
66	7832.5
67	6071.0
68	4834.0
69	3597.0
70	3071.0
71	2545.0
72	2031.0
73	1517.0
74	1462.0
75	1407.0
76	1056.5
77	706.0
78	518.0
79	330.0
80	250.0
81	170.0
82	122.0
83	74.0
84	56.0
85	38.0
86	21.0
87	4.0
88	2.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	607030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.293698072259424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.75079114930382	17.64255451631716
2	12.460602278559326	6.303494236647748
3	4.783570340486199	3.6298255169901936
4	2.3192746045240207	2.3465212637395783
5	1.263466647998832	1.5978871959426064
6	0.7792193592265334	1.1825603522601236
7	0.5398893220358412	0.9559058252807981
8	0.4270538575260783	0.8641417066286652
9	0.3216051795275443	0.7321125878500044
>10	6.423093264466628	44.30073737568714
>50	0.7925988380375577	12.680535214178152
>100	0.13101345924798444	5.31578772279099
>500	0.005866274294685871	1.1032860292440934
>1k	0.00195542476489529	1.3446504564427415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	3583	0.590250893695534	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	2631	0.43342174192379285	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1942	0.3199182906940349	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	997	0.16424229445002717	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	879	0.14480338698252146	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	830	0.13673129828838773	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	824	0.13574287926461623	Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	817	0.13458972373688285	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	708	0.11663344480503436	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4418233036258504	0.0
2	0.0	0.0	0.0	1.6094756437078892	0.0
3	0.0	0.0	0.0	2.494769615999209	0.0
4	0.0	0.0	0.0	3.0372469235457884	0.0
5	0.0	0.0	0.0	5.8697263726669195	0.0
6	0.0	0.0	0.0	6.306772317677874	0.0
7	0.0	0.0	0.0	7.986919921585424	0.0
8	0.0	0.0	0.0	9.899675469087194	0.0
9	0.0	0.0	0.0	11.436667051051842	0.0
10	0.0	0.0	0.0	12.419320297184653	0.0
11	0.0	0.0	0.0	13.401149860797654	0.0
12	0.0	0.0	0.0	14.306212213564404	0.0
13	0.0	0.0	0.0	14.672091988863812	0.0
14	0.0	0.0	0.0	14.95346193763076	0.0
15	0.0	0.0	0.0	15.886529496071034	0.0
16	0.0	0.0	0.0	16.916462118840915	0.0
17	0.0	0.0	0.0	18.50583990906545	0.0
18	0.0	0.0	0.0	19.103998154951157	0.0
19	0.0	0.0	0.0	19.93542329044693	0.0
20	0.0	0.0	0.0	20.80852676144507	0.0
21	0.0	0.0	0.0	21.52084740457638	0.0
22	0.0	0.0	0.0	22.368416717460423	0.0
23	1.647365039619129E-4	0.0	0.0	22.945653427342965	0.0
24	1.647365039619129E-4	0.0	0.0	23.430308221998914	0.0
25	1.647365039619129E-4	0.0	0.0	23.953346622077987	0.0
26	1.647365039619129E-4	0.0	0.0	24.384791525954235	0.0
27	1.647365039619129E-4	0.0	0.0	24.86318633345963	0.0
28	1.647365039619129E-4	0.0	0.0	25.298420176927007	0.0
29	1.647365039619129E-4	0.0	0.0	25.90843945109797	0.0
30	1.647365039619129E-4	0.0	0.0	26.518458725268932	0.0
31	1.647365039619129E-4	0.0	0.0	27.00228983740507	0.0
32	1.647365039619129E-4	0.0	0.0	27.45943363589938	0.0
33	1.647365039619129E-4	0.0	0.0	27.92382584056801	0.0
34	1.647365039619129E-4	0.0	0.0	28.61703704923974	0.0
35	1.647365039619129E-4	0.0	0.0	29.090160288618353	0.0
36	3.294730079238258E-4	0.0	0.0	29.574320873762417	0.0
37	3.294730079238258E-4	0.0	0.0	29.9965405334168	0.0
38	3.294730079238258E-4	0.0	0.0	30.41843072006326	0.0
39	3.294730079238258E-4	0.0	0.0	30.90605077179052	0.0
40	3.294730079238258E-4	0.0	0.0	31.401413439203992	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGAA	55	1.8189894E-12	46.000004	18
TTAGGCA	20	6.309089E-4	46.0	12
ACGCCCA	20	6.309089E-4	46.0	35
CGTATCT	20	6.309089E-4	46.0	34
GGCACCG	180	0.0	46.0	7
CATGACG	25	3.415237E-5	46.0	29
ATTACGT	20	6.309089E-4	46.0	21
GCTATCC	20	6.309089E-4	46.0	42
TTCGTAG	25	3.415237E-5	46.0	30
TACGGGT	40	5.6043064E-9	46.0	3
ACGGGTC	30	1.8599403E-6	46.0	4
GTTACGG	50	1.6370905E-11	46.0	1
AATGCGG	60	0.0	46.0	1
CCACTAC	20	6.309089E-4	46.0	15
ACCTAGG	40	5.6043064E-9	46.0	1
TACGCGG	60	0.0	46.0	1
AGGCCCG	25	3.415237E-5	46.0	32
CGCTATA	20	6.309089E-4	46.0	19
TGCGACA	25	3.415237E-5	46.0	20
AAACCGG	25	3.415237E-5	46.0	1
>>END_MODULE