FastQCFastQC Report
Thu 26 May 2016
SRR1544863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544863_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences465552
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT42130.9049472454204901Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT31110.6682389937106918Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT22340.4798604667147816Illumina Single End Adapter 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTTG16570.35592157267072205Illumina Single End Adapter 2 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA12680.27236484860982235No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT10170.2184503557067739Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC8690.18666013678386087Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC8510.18279375880674983Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC8360.17957177715915731Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT6120.13145685122177544Illumina Single End Adapter 2 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC5640.12114650994947934Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT5050.10847338213561535No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG4880.10482180293501049No Hit
GTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTTGA4810.1033182114994673Illumina Single End Adapter 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT206.307566E-446.013
ACGCCTC206.307566E-446.022
GGTAAGT301.8589999E-646.028
CATGCGG850.046.01
TCCGATG206.307566E-446.039
CCGATAG206.307566E-446.011
TTTCGAA253.414002E-546.045
TAAACGG405.5988494E-946.01
CCTACCG253.414002E-546.028
CACGAGG600.046.01
TAACGAG253.414002E-546.020
ATCACGG301.8589999E-646.01
ACGGGTA301.8589999E-646.04
AATGCGG501.6370905E-1146.01
CTAACGA253.414002E-546.019
ACCTAGG253.414002E-546.01
TACGCGG301.8589999E-646.01
CTAGTGG253.414002E-546.01
TAGGGTC206.307566E-446.04
AGCGAAC206.307566E-446.027
Produced by FastQC (version 0.11.5)