##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544863_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	465552
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91220959205416	34.0	33.0	34.0	31.0	34.0
2	33.247272055538375	34.0	33.0	34.0	31.0	34.0
3	33.337925731175034	34.0	34.0	34.0	31.0	34.0
4	36.57919845688559	37.0	37.0	37.0	35.0	37.0
5	36.578395109461454	37.0	37.0	37.0	35.0	37.0
6	36.52084622125992	37.0	37.0	37.0	35.0	37.0
7	36.394059095439395	37.0	37.0	37.0	35.0	37.0
8	36.44241030003093	37.0	37.0	37.0	35.0	37.0
9	38.28571459256968	39.0	39.0	39.0	37.0	39.0
10	38.103270955768636	39.0	39.0	39.0	35.0	39.0
11	37.33959901364402	39.0	35.0	39.0	35.0	39.0
12	37.290843987352645	39.0	35.0	39.0	35.0	39.0
13	37.28795279582088	39.0	35.0	39.0	35.0	39.0
14	38.49028679932639	40.0	35.0	41.0	35.0	41.0
15	38.55732120149844	41.0	35.0	41.0	35.0	41.0
16	38.57515164793622	41.0	35.0	41.0	35.0	41.0
17	38.65494509743272	41.0	36.0	41.0	35.0	41.0
18	38.645792521565795	40.0	36.0	41.0	35.0	41.0
19	38.54601848987868	40.0	35.0	41.0	35.0	41.0
20	38.48629583805891	40.0	35.0	41.0	35.0	41.0
21	38.43580094167783	40.0	35.0	41.0	35.0	41.0
22	38.40917233735436	40.0	35.0	41.0	35.0	41.0
23	38.388308932192324	40.0	35.0	41.0	35.0	41.0
24	38.311372306423344	40.0	35.0	41.0	35.0	41.0
25	38.29042083376293	40.0	35.0	41.0	35.0	41.0
26	38.23805504003849	40.0	35.0	41.0	35.0	41.0
27	38.25491030003093	40.0	35.0	41.0	35.0	41.0
28	38.314310753685945	40.0	35.0	41.0	35.0	41.0
29	38.328970770182494	40.0	35.0	41.0	35.0	41.0
30	38.2614229989346	40.0	35.0	41.0	35.0	41.0
31	38.217305478228	40.0	35.0	41.0	35.0	41.0
32	38.16262200570505	40.0	35.0	41.0	35.0	41.0
33	38.10875047255731	40.0	35.0	41.0	35.0	41.0
34	38.03654586383476	40.0	35.0	41.0	35.0	41.0
35	37.960195638725644	40.0	35.0	41.0	35.0	41.0
36	37.914748513592464	40.0	35.0	41.0	35.0	41.0
37	37.851679296834725	40.0	35.0	41.0	35.0	41.0
38	37.82057428600886	40.0	35.0	41.0	35.0	41.0
39	37.76825574801526	40.0	35.0	41.0	35.0	41.0
40	37.73707985359315	40.0	35.0	41.0	35.0	41.0
41	37.71357871945561	40.0	35.0	41.0	35.0	41.0
42	37.650709265560025	40.0	35.0	41.0	35.0	41.0
43	37.56857021342407	39.0	35.0	41.0	35.0	41.0
44	37.43822816785236	39.0	35.0	41.0	35.0	41.0
45	37.438567549919235	39.0	35.0	41.0	35.0	41.0
46	37.39866008523215	39.0	35.0	41.0	35.0	41.0
47	37.32460176306836	39.0	35.0	41.0	35.0	41.0
48	37.3120145547651	39.0	35.0	41.0	35.0	41.0
49	37.26359246657731	38.0	35.0	41.0	35.0	41.0
50	37.1829999656322	38.0	35.0	41.0	35.0	41.0
51	37.11275217376362	38.0	35.0	41.0	34.0	41.0
52	36.73591564422449	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	5.0
15	8.0
16	9.0
17	12.0
18	26.0
19	59.0
20	110.0
21	170.0
22	245.0
23	447.0
24	773.0
25	1262.0
26	1758.0
27	1958.0
28	1894.0
29	2107.0
30	2592.0
31	3304.0
32	4408.0
33	6454.0
34	21725.0
35	109449.0
36	15668.0
37	24980.0
38	42882.0
39	219664.0
40	3579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.034342028387808	21.412430834793966	24.188919819912705	23.36430731690552
2	25.506065917448534	24.579209196824415	27.0438103584562	22.870914527270852
3	26.588007354710108	24.78348283328178	26.19599958758635	22.432510224421762
4	26.745669656665637	25.9784084269856	23.913547788431796	23.36237412791697
5	24.73751589510946	33.228726329174826	20.86319036326769	21.17056741244802
6	84.04796886276937	2.7047461937656805	9.172766951919442	4.0745179915455205
7	85.63597621747947	2.362142145238341	6.522364848609823	5.479516788672371
8	74.56868405677561	4.373517888442108	13.267905626009554	7.789892428772725
9	52.16968244148882	17.23996460116163	14.703620648176788	15.886732309172766
10	43.95126645358628	18.86942811973743	24.42369488263395	12.755610544042343
11	38.990273911399804	17.779539127745128	24.498659655634604	18.731527305220467
12	19.281412173076262	35.417955459325704	27.792813692133212	17.507818675464826
13	13.168668591263705	38.86032065161357	30.164621782314327	17.8063889748084
14	11.563692133209608	23.48223184520741	46.25584252672097	18.698233494862013
15	12.935182321201498	17.283568752792384	48.64419012269306	21.137058803313057
16	13.741751726982162	21.487395607794618	27.20705742860089	37.56379523662233
17	18.528327662645633	22.733443310306907	33.40593531979242	25.332293707255044
18	24.40672577928996	26.06604632780012	27.40424270543355	22.12298518747637
19	26.425619479671443	21.838376808605698	25.03544179812352	26.70056191359934
20	19.095181633845414	29.951756194796715	27.969593085197786	22.983469086160085
21	19.58814482592707	24.549566965666564	26.318005292641857	29.544282915764512
22	17.567317936557032	29.83597965425989	23.132539437055367	29.46416297212771
23	17.928824277416915	21.84331718046534	38.268335223562566	21.959523318555178
24	19.350362580334743	21.767493212358662	34.27629996219542	24.60584424511118
25	15.92131491219026	31.607210365329756	27.91288620820016	24.558588514279823
26	17.447675018043096	28.615493006151837	28.514537581193938	25.42229439461113
27	20.589107124445817	24.700355706773895	36.04667147815926	18.663865690621027
28	15.882865931195656	23.383424408014573	31.549214695673093	29.18449496511668
29	18.289041825617762	25.020835481321097	30.272665566896933	26.417457126164205
30	29.458148606385535	23.848678557926934	20.72980032305736	25.963372512630166
31	26.948010104134447	24.747396638828747	22.52852527752002	25.776067979516785
32	25.27193525105681	25.559121215245558	23.291490531669933	25.8774530020277
33	19.002173763618245	24.614865793724437	25.732893425439045	30.65006701721827
34	19.18690071141355	25.024487060521704	27.432596143932365	28.356016084132385
35	29.299197511770974	19.67191634876448	24.185053441935594	26.843832697528956
36	20.86727154002131	29.55674124480187	24.182475856617522	25.3935113585593
37	21.95028697116541	27.755438705021135	24.859951197717976	25.434323126095475
38	20.296980788397427	28.862941196686943	22.768240712100905	28.07183730281472
39	26.399199230161187	23.96552909234629	24.699711310444375	24.935560367048147
40	17.77996872529814	21.215030759184796	29.636001993332645	31.368998522184416
41	22.591461319036327	25.707976767364332	22.989483451902256	28.71107846169708
42	23.495764168127298	21.184958930473933	26.726122968003573	28.593153933395193
43	23.412637041619412	25.26076571467849	26.10556930267725	25.221027941024847
44	17.55335601608413	28.764348558270612	24.692408152043164	28.98988727360209
45	22.19215039351136	25.107184589476578	25.463965357253326	27.236699659758738
46	24.768661717702855	22.524658899542906	26.265164793621338	26.441514589132904
47	22.20503832010173	19.445088840773963	31.75628071622504	26.593592122899267
48	19.867812832938103	18.610165996494484	28.668118706395852	32.85390246417156
49	20.43595559679692	23.639464549609926	30.4735024229302	25.451077430662956
50	19.436067292160704	25.09472626043922	27.551379867340277	27.917826580059803
51	20.373019555280614	20.404809774203525	29.31165584080833	29.91051482970753
52	22.48492112588927	18.957495618104957	33.770019245970374	24.7875640100354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	130.5
2	150.0
3	525.5
4	901.0
5	673.5
6	446.0
7	507.5
8	569.0
9	620.0
10	671.0
11	690.0
12	709.0
13	660.5
14	592.5
15	573.0
16	546.0
17	519.0
18	558.5
19	598.0
20	645.5
21	693.0
22	673.0
23	653.0
24	703.5
25	754.0
26	1005.5
27	1257.0
28	1446.5
29	1636.0
30	2007.0
31	2378.0
32	2901.5
33	3425.0
34	4427.0
35	5429.0
36	5830.0
37	6231.0
38	7068.5
39	9110.5
40	10315.0
41	15957.0
42	21599.0
43	28293.5
44	34988.0
45	38628.5
46	42269.0
47	43591.5
48	44914.0
49	52113.0
50	59312.0
51	60129.0
52	60946.0
53	53122.5
54	45299.0
55	37312.0
56	29325.0
57	25031.5
58	20738.0
59	18603.5
60	16469.0
61	14733.0
62	12997.0
63	12206.0
64	9246.0
65	7077.0
66	5644.0
67	4211.0
68	3408.0
69	2605.0
70	2205.5
71	1806.0
72	1466.0
73	1126.0
74	1067.5
75	1009.0
76	757.0
77	505.0
78	358.5
79	212.0
80	160.0
81	108.0
82	85.0
83	62.0
84	42.0
85	22.0
86	12.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	465552.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.774003877776487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.07608601725568	20.163695707999135
2	12.21454373526772	7.029226576077277
3	4.450467604272532	3.8417331630976945
4	2.1015185044757914	2.418764063880218
5	1.1516669837952431	1.656903512881574
6	0.7942406204477426	1.3712089615590568
7	0.5922918402789306	1.1929825396272922
8	0.46799492493523365	1.0772870227892906
9	0.396181473436945	1.0259754527680072
>10	7.372303827466966	46.32567108466805
>50	0.2825435332867391	5.378214219925056
>100	0.09119129910312744	4.703517826743223
>500	0.004484817988678398	0.9112810385436481
>1k	0.004484817988678398	2.9035388294404645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	4213	0.9049472454204901	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	3111	0.6682389937106918	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	2234	0.4798604667147816	Illumina Single End Adapter 2 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTTG	1657	0.35592157267072205	Illumina Single End Adapter 2 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1268	0.27236484860982235	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1017	0.2184503557067739	Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	869	0.18666013678386087	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	851	0.18279375880674983	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	836	0.17957177715915731	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	612	0.13145685122177544	Illumina Single End Adapter 2 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	564	0.12114650994947934	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	505	0.10847338213561535	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG	488	0.10482180293501049	No Hit
GTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTTGA	481	0.1033182114994673	Illumina Single End Adapter 2 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6796233288655188	0.0
2	0.0	0.0	0.0	2.441402893769117	0.0
3	0.0	0.0	0.0	3.6724146819259715	0.0
4	0.0	0.0	0.0	4.45299343574939	0.0
5	0.0	0.0	0.0	8.35932398529058	0.0
6	0.0	0.0	0.0	8.952383407224113	0.0
7	0.0	0.0	0.0	11.3405162044197	0.0
8	0.0	0.0	0.0	13.989629515070282	0.0
9	0.0	0.0	0.0	16.108834244080146	0.0
10	0.0	0.0	0.0	17.415455201567173	0.0
11	0.0	0.0	0.0	18.710477025122866	0.0
12	0.0	0.0	0.0	19.95437673987009	0.0
13	0.0	0.0	0.0	20.448199127057773	0.0
14	0.0	0.0	0.0	20.81743822387188	0.0
15	0.0	0.0	0.0	22.0020534763034	0.0
16	0.0	0.0	0.0	23.274521428325944	0.0
17	0.0	0.0	0.0	25.04489294428979	0.0
18	0.0	0.0	0.0	25.749218132453517	0.0
19	0.0	0.0	0.0	26.701635907481872	0.0
20	0.0	0.0	0.0	27.735462418806062	0.0
21	0.0	0.0	0.0	28.56651888510843	0.0
22	0.0	0.0	0.0	29.49681238615665	0.0
23	2.1479877650616903E-4	0.0	0.0	30.14206791078118	0.0
24	2.1479877650616903E-4	0.0	0.0	30.699685534591193	0.0
25	2.1479877650616903E-4	0.0	0.0	31.274487060521704	0.0
26	2.1479877650616903E-4	0.0	0.0	31.760361892978658	0.0
27	2.1479877650616903E-4	0.0	0.0	32.271368182286835	0.0
28	2.1479877650616903E-4	0.0	0.0	32.74113310650583	0.0
29	2.1479877650616903E-4	0.0	0.0	33.41839364882978	0.0
30	2.1479877650616903E-4	0.0	0.0	34.0320737533079	0.0
31	2.1479877650616903E-4	0.0	0.0	34.560049145960065	0.0
32	2.1479877650616903E-4	0.0	0.0	35.06074509399595	0.0
33	2.1479877650616903E-4	0.0	0.0	35.554782279960136	0.0
34	2.1479877650616903E-4	0.0	0.0	36.24149396845036	0.0
35	2.1479877650616903E-4	0.0	0.0	36.77462453173867	0.0
36	2.1479877650616903E-4	0.0	0.0	37.28176444306973	0.0
37	2.1479877650616903E-4	0.0	0.0	37.72833109942606	0.0
38	2.1479877650616903E-4	0.0	0.0	38.17232017046431	0.0
39	2.1479877650616903E-4	0.0	0.0	38.654113826167645	0.0
40	2.1479877650616903E-4	0.0	0.0	39.17371206653607	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	20	6.307566E-4	46.0	13
ACGCCTC	20	6.307566E-4	46.0	22
GGTAAGT	30	1.8589999E-6	46.0	28
CATGCGG	85	0.0	46.0	1
TCCGATG	20	6.307566E-4	46.0	39
CCGATAG	20	6.307566E-4	46.0	11
TTTCGAA	25	3.414002E-5	46.0	45
TAAACGG	40	5.5988494E-9	46.0	1
CCTACCG	25	3.414002E-5	46.0	28
CACGAGG	60	0.0	46.0	1
TAACGAG	25	3.414002E-5	46.0	20
ATCACGG	30	1.8589999E-6	46.0	1
ACGGGTA	30	1.8589999E-6	46.0	4
AATGCGG	50	1.6370905E-11	46.0	1
CTAACGA	25	3.414002E-5	46.0	19
ACCTAGG	25	3.414002E-5	46.0	1
TACGCGG	30	1.8589999E-6	46.0	1
CTAGTGG	25	3.414002E-5	46.0	1
TAGGGTC	20	6.307566E-4	46.0	4
AGCGAAC	20	6.307566E-4	46.0	27
>>END_MODULE