FastQCFastQC Report
Thu 26 May 2016
SRR1544862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544862_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences693359
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT35980.5189230975584077RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT29200.4211382559395638TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT18860.272009161199321RNA PCR Primer, Index 35 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC8800.12691837850233428RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8420.12143781215791531No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT8400.12114936129768274RNA PCR Primer, Index 35 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC8120.11711104925442664TruSeq Adapter, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC7130.10283273167291404RNA PCR Primer, Index 35 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACG1050.046.00000423
CGCCCTA750.046.00000416
GTGCAAC206.3097134E-446.07
AGCAATC253.4157427E-546.032
AGGTAGT206.3097134E-446.040
AACCGGT206.3097134E-446.037
ATCTCGG405.6061253E-946.01
ACACGTA301.8603278E-646.010
TCCGCAA650.046.031
CGAGTTT206.3097134E-446.028
GTACACT253.4157427E-546.017
CGACGGT405.6061253E-946.027
TCCGAGA206.3097134E-446.020
CAGTTAT253.4157427E-546.045
GTGGGCG453.092282E-1046.04
CGTGAAT206.3097134E-446.030
ATCAGCA206.3097134E-446.031
GCTATCT253.4157427E-546.08
TTGTCAG206.3097134E-446.038
ACATTGC206.3097134E-446.043
Produced by FastQC (version 0.11.5)