##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544862_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	693359
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.944167451493385	34.0	33.0	34.0	31.0	34.0
2	33.23922527867959	34.0	33.0	34.0	31.0	34.0
3	33.27821662371153	34.0	34.0	34.0	31.0	34.0
4	36.563360394831534	37.0	37.0	37.0	35.0	37.0
5	36.55220167330344	37.0	37.0	37.0	35.0	37.0
6	36.681107478232775	37.0	37.0	37.0	35.0	37.0
7	36.668691110954065	37.0	37.0	37.0	35.0	37.0
8	36.587578729056666	37.0	37.0	37.0	35.0	37.0
9	38.37047763135691	39.0	39.0	39.0	37.0	39.0
10	38.030900298402415	39.0	39.0	39.0	35.0	39.0
11	36.78549496004234	38.0	35.0	39.0	35.0	39.0
12	36.60357332925656	38.0	35.0	39.0	35.0	39.0
13	36.56980438704913	38.0	35.0	39.0	35.0	39.0
14	37.62459418569601	39.0	35.0	41.0	35.0	41.0
15	37.732559900426764	39.0	35.0	41.0	35.0	41.0
16	37.76067664802793	39.0	35.0	41.0	35.0	41.0
17	37.89175448793482	39.0	35.0	41.0	35.0	41.0
18	37.88631574696514	39.0	35.0	41.0	35.0	41.0
19	37.730938806592256	38.0	35.0	41.0	35.0	41.0
20	37.608612565784824	38.0	35.0	41.0	35.0	41.0
21	37.53587102785137	38.0	35.0	41.0	35.0	41.0
22	37.53052170664836	38.0	35.0	41.0	35.0	41.0
23	37.50190017004178	38.0	35.0	41.0	35.0	41.0
24	37.463272273093736	38.0	35.0	41.0	35.0	41.0
25	37.42496455660055	37.0	35.0	41.0	35.0	41.0
26	37.38270794783078	37.0	35.0	41.0	35.0	41.0
27	37.427575036885656	37.0	35.0	41.0	35.0	41.0
28	37.43938421510357	37.0	35.0	41.0	35.0	41.0
29	37.46507509097019	37.0	35.0	41.0	35.0	41.0
30	37.33015508560501	37.0	35.0	41.0	35.0	41.0
31	37.19430915297847	37.0	35.0	41.0	35.0	41.0
32	37.15400968329538	37.0	35.0	41.0	35.0	41.0
33	37.05821515261214	36.0	35.0	41.0	35.0	41.0
34	36.94638708086287	36.0	35.0	41.0	34.0	41.0
35	36.872005699789	36.0	35.0	41.0	35.0	41.0
36	36.84634655351701	36.0	35.0	41.0	34.0	41.0
37	36.79590803609674	36.0	35.0	41.0	34.0	41.0
38	36.78257583733679	36.0	35.0	41.0	34.0	41.0
39	36.680203184785945	36.0	35.0	41.0	34.0	41.0
40	36.67167080834027	36.0	35.0	41.0	34.0	41.0
41	36.62083134422428	36.0	35.0	41.0	34.0	41.0
42	36.50358183855694	35.0	35.0	41.0	34.0	41.0
43	36.41835182062972	35.0	35.0	41.0	34.0	41.0
44	36.40302642642556	35.0	35.0	41.0	34.0	41.0
45	36.409731466671666	35.0	35.0	41.0	34.0	41.0
46	36.368614238799815	35.0	35.0	41.0	34.0	41.0
47	36.30312147098401	35.0	35.0	40.0	34.0	41.0
48	36.33372466500038	36.0	35.0	40.0	34.0	41.0
49	36.2547453772144	35.0	35.0	40.0	34.0	41.0
50	36.16301511915184	35.0	35.0	40.0	34.0	41.0
51	36.08528482359066	36.0	35.0	40.0	33.0	41.0
52	35.87578873282095	35.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	1.0
14	1.0
15	1.0
16	6.0
17	19.0
18	67.0
19	116.0
20	195.0
21	383.0
22	628.0
23	982.0
24	1611.0
25	2631.0
26	3706.0
27	4308.0
28	4244.0
29	4522.0
30	5297.0
31	6549.0
32	8824.0
33	13425.0
34	46128.0
35	235439.0
36	21652.0
37	29323.0
38	54025.0
39	247532.0
40	1741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.757888770463786	22.741465820736444	25.648185139300132	21.852460269499637
2	24.948836028666246	24.85581062624124	27.702099489586203	22.49325385550631
3	24.799562708495888	25.484777726978376	27.17798427654361	22.53767528798213
4	25.592946799565592	28.141265924290305	24.53202453563017	21.73376274051393
5	24.473324785572842	34.03936488889594	21.217724151557853	20.269586173973366
6	88.31384607396745	2.5112531891848233	6.58316975765801	2.5917309791897125
7	89.59240451194836	1.7820494145168664	4.735497772438232	3.8900483010965456
8	81.90158345099725	3.297858685039064	9.378114367881574	5.422443496082116
9	61.611661491377475	13.855881296701996	12.171616723803973	12.360840488116546
10	56.90010514033855	15.242608807270116	18.48018126252057	9.377104789870758
11	51.332715086989566	15.952630599732606	19.05347734723282	13.661176966045009
12	18.187259413954386	46.54486348341912	21.82664391750882	13.441233185117666
13	11.097137269437622	49.42821828230397	25.464009265041632	14.010635183216774
14	9.55969418439798	21.24036754408611	55.549001311009164	13.650936960506751
15	10.554705426770258	14.075103950478757	57.21076671680903	18.159423905941942
16	11.411692932521248	16.291848811366116	26.48930784773833	45.807150408374305
17	18.264564244496718	20.698079926848862	37.38467374044326	23.652682088211158
18	27.31240237741199	25.273775922718244	25.121473868515444	22.29234783135432
19	31.82838904521323	20.78014419658503	23.247985531304852	24.14348122689689
20	18.302495532617304	30.755207619717922	27.233799517998612	23.708497329666162
21	20.366938339301864	23.82474302633989	24.7244212594053	31.083897374952947
22	15.911093675859114	32.80017999333678	20.91297581772213	30.375750513081968
23	17.698912107580632	21.024894751492372	41.70292734355507	19.573265797371924
24	15.459379628734899	20.67284047657851	39.700501471820516	24.167278422866076
25	13.889918498209441	36.31351147096959	27.501770367154677	22.294799663666296
26	16.782359499191614	32.73383629548329	27.430234553816994	23.053569651508095
27	16.55304106530672	27.107890717507093	40.9833866727049	15.355681544481287
28	13.33032382935824	22.11249871999931	39.700357246390396	24.856820204252053
29	18.609118797044534	28.087469838856926	31.832283131826372	21.47112823227217
30	32.59307227568979	23.87724108290222	22.094326315804654	21.43536032560333
31	32.28572788411198	24.9032607927495	25.30492861562336	17.506082707515155
32	29.535637382654585	26.375658208806698	24.752256767417744	19.336447641120976
33	19.296641422408882	25.37761823240197	29.45948635555318	25.86625398963596
34	19.67379092216298	22.063606299189885	33.507317277196954	24.755285501450185
35	31.445758979114714	17.637183623490863	32.0264105607629	18.89064683663153
36	22.088124622309653	25.49775801568884	31.534024942345884	20.880092419655618
37	21.716167238039745	28.185975807626352	27.194425975576863	22.903430978757036
38	18.77209353307594	26.889821867171264	30.07475204042927	24.263332559323526
39	26.064708181475975	21.616363240399274	25.88529174641131	26.433636831713443
40	16.672892397733353	17.977122962274954	30.99866014575422	34.351324494237474
41	24.638318677625875	22.590173344544457	23.775994830960585	28.995513146869083
42	23.622106297026505	21.650111991046487	27.421581028010024	27.306200683916988
43	22.443640307546307	26.653724838070897	29.397757871463416	21.50487698291938
44	16.625009554934746	34.67020692022459	24.83533061516473	23.86945290967594
45	23.427113515509284	28.610287023028473	27.94584046648273	20.016758994979515
46	25.192432780132656	22.479119763354916	27.26451953461338	25.063927921899044
47	24.605723730419594	17.19801718878676	34.185465249603745	24.010793831189904
48	20.017047445839744	15.61009520320642	33.5378930683816	30.83496428257223
49	20.184637395634873	26.552190135269033	30.646461645410245	22.616710823685853
50	18.00668917544879	30.09739543295753	25.790679864255022	26.10523552733865
51	20.70471429663421	21.253924734517042	31.47503674142832	26.56632422742043
52	24.97753688925939	18.968817019754557	32.11727258173616	23.936373509249897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	168.5
2	219.0
3	862.5
4	1506.0
5	1140.5
6	775.0
7	905.0
8	1035.0
9	1155.5
10	1276.0
11	1247.0
12	1218.0
13	1171.0
14	1047.0
15	970.0
16	940.5
17	911.0
18	874.5
19	838.0
20	947.5
21	1057.0
22	1055.5
23	1054.0
24	1000.5
25	947.0
26	1304.0
27	1661.0
28	1973.0
29	2285.0
30	2512.0
31	2739.0
32	3273.0
33	3807.0
34	5404.5
35	7002.0
36	7966.5
37	8931.0
38	10146.0
39	14686.5
40	18012.0
41	29879.5
42	41747.0
43	62207.5
44	82668.0
45	90165.5
46	97663.0
47	94271.0
48	90879.0
49	87286.5
50	83694.0
51	72754.5
52	61815.0
53	53441.5
54	45068.0
55	37927.5
56	30787.0
57	26756.0
58	22725.0
59	20918.5
60	19112.0
61	18484.5
62	17857.0
63	14527.5
64	9298.5
65	7399.0
66	5707.0
67	4015.0
68	3555.5
69	3096.0
70	2497.0
71	1898.0
72	1385.5
73	873.0
74	793.0
75	713.0
76	722.5
77	732.0
78	503.0
79	274.0
80	238.0
81	202.0
82	126.5
83	51.0
84	48.0
85	45.0
86	23.0
87	1.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	693359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.589609946342986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.56822930587616	14.117930961193945
2	12.882785700442994	5.3050306518889245
3	5.3397054753183735	3.2982735889547183
4	2.709900727928496	2.231831959254346
5	1.5682761078682965	1.6145096674588573
6	0.9731569058967082	1.2022152665401937
7	0.5629429883380508	0.8113543586336421
8	0.46141148952198513	0.7600226075215037
9	0.3609930476480368	0.6689435439973216
>10	3.6510732932718852	19.385134262006126
>50	2.5199817098989907	36.043066747691526
>100	0.393834738866305	12.514837501011892
>500	0.005606188453612882	0.8342647854506866
>1k	0.002102320670104831	1.2125840983963283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	3598	0.5189230975584077	RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT	2920	0.4211382559395638	TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	1886	0.272009161199321	RNA PCR Primer, Index 35 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	880	0.12691837850233428	RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	842	0.12143781215791531	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	840	0.12114936129768274	RNA PCR Primer, Index 35 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	812	0.11711104925442664	TruSeq Adapter, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	713	0.10283273167291404	RNA PCR Primer, Index 35 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.431234036047704	0.0
2	0.0	0.0	0.0	1.4573979713251	0.0
3	0.0	0.0	0.0	2.2507820623948054	0.0
4	0.0	0.0	0.0	2.7262933054882104	0.0
5	0.0	0.0	0.0	5.095195995148257	0.0
6	0.0	0.0	0.0	5.534650880712589	0.0
7	0.0	0.0	0.0	7.378428779319227	0.0
8	0.0	0.0	0.0	9.452390464391462	0.0
9	0.0	0.0	0.0	11.279293987674494	0.0
10	0.0	0.0	0.0	12.21026913907514	0.0
11	0.0	0.0	0.0	13.109803146710433	0.0
12	0.0	0.0	0.0	13.972703895096192	0.0
13	0.0	0.0	0.0	14.261010529898654	0.0
14	1.4422543011628896E-4	0.0	0.0	14.495376853837623	0.0
15	1.4422543011628896E-4	0.0	0.0	15.341403226899773	0.0
16	1.4422543011628896E-4	0.0	0.0	16.234879766470183	0.0
17	1.4422543011628896E-4	0.0	0.0	17.73943945344331	0.0
18	1.4422543011628896E-4	0.0	0.0	18.23297887530125	0.0
19	1.4422543011628896E-4	0.0	0.0	18.894252472384437	0.0
20	1.4422543011628896E-4	0.0	0.0	19.677252332485768	0.0
21	1.4422543011628896E-4	0.0	0.0	20.245500527143946	0.0
22	1.4422543011628896E-4	0.0	0.0	21.046961242300164	0.0
23	1.4422543011628896E-4	0.0	0.0	21.533000941792057	0.0
24	1.4422543011628896E-4	0.0	0.0	21.910006216116038	0.0
25	1.4422543011628896E-4	0.0	0.0	22.33475010780851	0.0
26	1.4422543011628896E-4	0.0	0.0	22.644257880838065	0.0
27	1.4422543011628896E-4	0.0	0.0	23.04981979032507	0.0
28	1.4422543011628896E-4	0.0	0.0	23.416585059110794	0.0
29	1.4422543011628896E-4	0.0	0.0	24.01685129925479	0.0
30	2.884508602325779E-4	0.0	0.0	24.563754130255756	0.0
31	2.884508602325779E-4	0.0	0.0	24.968739138022293	0.0
32	2.884508602325779E-4	0.0	0.0	25.325985528420343	0.0
33	2.884508602325779E-4	0.0	0.0	25.708038692798393	0.0
34	2.884508602325779E-4	0.0	0.0	26.359504960633668	0.0
35	2.884508602325779E-4	0.0	0.0	26.764922644690557	0.0
36	2.884508602325779E-4	0.0	0.0	27.18216681401698	0.0
37	2.884508602325779E-4	0.0	0.0	27.53349996178026	0.0
38	2.884508602325779E-4	0.0	0.0	27.846180694272377	0.0
39	2.884508602325779E-4	0.0	0.0	28.289241215589616	0.0
40	2.884508602325779E-4	0.0	0.0	28.721340604218017	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACACG	105	0.0	46.000004	23
CGCCCTA	75	0.0	46.000004	16
GTGCAAC	20	6.3097134E-4	46.0	7
AGCAATC	25	3.4157427E-5	46.0	32
AGGTAGT	20	6.3097134E-4	46.0	40
AACCGGT	20	6.3097134E-4	46.0	37
ATCTCGG	40	5.6061253E-9	46.0	1
ACACGTA	30	1.8603278E-6	46.0	10
TCCGCAA	65	0.0	46.0	31
CGAGTTT	20	6.3097134E-4	46.0	28
GTACACT	25	3.4157427E-5	46.0	17
CGACGGT	40	5.6061253E-9	46.0	27
TCCGAGA	20	6.3097134E-4	46.0	20
CAGTTAT	25	3.4157427E-5	46.0	45
GTGGGCG	45	3.092282E-10	46.0	4
CGTGAAT	20	6.3097134E-4	46.0	30
ATCAGCA	20	6.3097134E-4	46.0	31
GCTATCT	25	3.4157427E-5	46.0	8
TTGTCAG	20	6.3097134E-4	46.0	38
ACATTGC	20	6.3097134E-4	46.0	43
>>END_MODULE