FastQCFastQC Report
Thu 26 May 2016
SRR1544861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544861_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences470024
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT34450.732941296614641RNA PCR Primer, Index 10 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT27560.5863530372917127TruSeq Adapter, Index 10 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT18800.39997957551103774RNA PCR Primer, Index 35 (96% over 25bp)
TGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTTG12800.27232651949687675RNA PCR Primer, Index 35 (96% over 27bp)
ACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC7980.1697785644988341RNA PCR Primer, Index 35 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT7900.16807652375197862RNA PCR Primer, Index 35 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC6690.14233315745578948TruSeq Adapter, Index 10 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC6560.13956734124214934RNA PCR Primer, Index 35 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6550.13935458614879243No Hit
AGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC5810.1236107092403792TruSeq Adapter, Index 10 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT4950.10531377121168281RNA PCR Primer, Index 35 (96% over 25bp)
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCG4740.10084591425118719No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGA206.307627E-446.00000441
GTCATCG206.307627E-446.00000443
TCGTTTC405.5988494E-946.00000440
GATACTA206.307627E-446.00000433
ATCTCGG206.307627E-446.0000041
GATACAC206.307627E-446.00000414
GGTACGG206.307627E-446.0000041
AGTCCAA206.307627E-446.00000426
TGCCCGC206.307627E-446.00000413
GTTCTAG206.307627E-446.00000410
CGAGTTA206.307627E-446.00000446
GATCGCC206.307627E-446.00000414
ACGTTTC206.307627E-446.00000419
GGGTACA206.307627E-446.0000046
CGACCGG206.307627E-446.00000432
TTCGTTT405.5988494E-946.00000439
TACGGGT206.307627E-446.0000043
ATCACGG551.8189894E-1246.0000041
ACGGGTA405.5988494E-946.0000044
ATACTAA206.307627E-446.00000434
Produced by FastQC (version 0.11.5)