FastQCFastQC Report
Thu 26 May 2016
SRR1544860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544860_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1239469
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT55510.44785307256575196RNA PCR Primer, Index 34 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT48140.3883921259829814RNA PCR Primer, Index 34 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT38040.30690561845435427TruSeq Adapter, Index 18 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24920.2010538383775633No Hit
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT17590.1419156106364903TruSeq Adapter, Index 18 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC16190.13062045117707666TruSeq Adapter, Index 3 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC15650.12626374681415994RNA PCR Primer, Index 34 (96% over 25bp)
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT14310.11545266561729256No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14100.11375839169838052No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA206.311646E-446.00000420
ACCGGTA351.0196527E-746.00000441
GTATTCG351.0196527E-746.00000411
CGTGATA206.311646E-446.00000430
CCGGATA206.311646E-446.00000410
ATCGTAC405.6115823E-946.00000433
GTTACGA206.311646E-446.00000435
CTAACGG1400.046.0000041
CCGTAGG750.046.0000041
CCGTAAG206.311646E-446.00000422
CGCTAAT206.311646E-446.00000427
TATTCGA351.0196527E-746.00000412
ACAGCGA206.311646E-446.00000423
CTCGCTA206.311646E-446.00000425
CGTACTC206.311646E-446.00000431
CGTACCC206.311646E-446.00000419
CGTACAA405.6115823E-946.00000435
CCGCGTA206.311646E-446.00000419
CGGTCGT206.311646E-446.00000427
TAAATCG206.311646E-446.00000410
Produced by FastQC (version 0.11.5)