##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544860_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1239469
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.883061214116694	34.0	33.0	34.0	31.0	34.0
2	33.21642897079314	34.0	33.0	34.0	31.0	34.0
3	33.270336732907396	34.0	34.0	34.0	31.0	34.0
4	36.55912814277727	37.0	37.0	37.0	35.0	37.0
5	36.55822937080314	37.0	37.0	37.0	35.0	37.0
6	36.668508853388026	37.0	37.0	37.0	35.0	37.0
7	36.64706579995143	37.0	37.0	37.0	35.0	37.0
8	36.55088913074873	37.0	37.0	37.0	35.0	37.0
9	38.32326181614869	39.0	39.0	39.0	37.0	39.0
10	38.094027361716996	39.0	39.0	39.0	35.0	39.0
11	37.27927765841663	39.0	35.0	39.0	35.0	39.0
12	37.165157821615544	39.0	35.0	39.0	35.0	39.0
13	37.14787622764264	39.0	35.0	39.0	35.0	39.0
14	38.39758799937715	40.0	35.0	41.0	35.0	41.0
15	38.482523564526424	40.0	35.0	41.0	35.0	41.0
16	38.4716261560394	40.0	35.0	41.0	35.0	41.0
17	38.54674300043002	40.0	36.0	41.0	35.0	41.0
18	38.50463141877691	40.0	36.0	41.0	35.0	41.0
19	38.40189790950802	40.0	35.0	41.0	35.0	41.0
20	38.31735364095431	40.0	35.0	41.0	35.0	41.0
21	38.26966305732535	40.0	35.0	41.0	35.0	41.0
22	38.253680406690286	40.0	35.0	41.0	35.0	41.0
23	38.212053710096825	40.0	35.0	41.0	35.0	41.0
24	38.16430745746767	40.0	35.0	41.0	35.0	41.0
25	38.131656378658924	40.0	35.0	41.0	35.0	41.0
26	38.06945796950146	40.0	35.0	41.0	35.0	41.0
27	38.03066877832362	40.0	35.0	41.0	35.0	41.0
28	38.07079160511477	40.0	35.0	41.0	35.0	41.0
29	38.06585077964838	40.0	35.0	41.0	35.0	41.0
30	37.94218169232147	40.0	35.0	41.0	35.0	41.0
31	37.88907427293462	40.0	35.0	41.0	35.0	41.0
32	37.78795839185974	40.0	35.0	41.0	35.0	41.0
33	37.69514929377016	40.0	35.0	41.0	35.0	41.0
34	37.610308123882085	40.0	35.0	41.0	35.0	41.0
35	37.530987059781246	40.0	35.0	41.0	35.0	41.0
36	37.46580108094676	39.0	35.0	41.0	34.0	41.0
37	37.40627317020434	39.0	35.0	41.0	34.0	41.0
38	37.38711819335538	39.0	35.0	41.0	34.0	41.0
39	37.273622010715876	39.0	35.0	41.0	34.0	41.0
40	37.21289358588234	39.0	35.0	41.0	34.0	41.0
41	37.1776002465572	39.0	35.0	41.0	34.0	41.0
42	37.07258108109198	38.0	35.0	41.0	34.0	41.0
43	37.05886875750826	38.0	35.0	41.0	34.0	41.0
44	37.00334014001157	38.0	35.0	41.0	34.0	41.0
45	36.97011058767908	38.0	35.0	41.0	34.0	41.0
46	36.923332491575025	38.0	35.0	41.0	34.0	41.0
47	36.82741561103989	38.0	35.0	41.0	34.0	41.0
48	36.82289593366192	37.0	35.0	41.0	34.0	41.0
49	36.7576470246533	37.0	35.0	41.0	34.0	41.0
50	36.658314971975905	37.0	35.0	41.0	34.0	41.0
51	36.5806357399822	37.0	35.0	41.0	34.0	41.0
52	36.32827283296315	36.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	1.0
14	2.0
15	5.0
16	18.0
17	30.0
18	66.0
19	176.0
20	364.0
21	566.0
22	901.0
23	1546.0
24	2647.0
25	4382.0
26	6071.0
27	6873.0
28	7260.0
29	7927.0
30	9583.0
31	11592.0
32	15125.0
33	21853.0
34	67278.0
35	285438.0
36	47563.0
37	73323.0
38	125194.0
39	539928.0
40	3753.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.262606003054536	22.16941286954333	25.445977269298385	20.122003858103753
2	27.261674152399134	24.362771477140612	25.7090738049923	22.666480565467957
3	26.362740818850654	24.185921551890367	26.297067534565205	23.15427009469378
4	26.385250458059055	26.869490080026203	23.317162430040604	23.428097031874135
5	24.426105049823754	34.990225653082085	21.02529389601515	19.55837540107901
6	86.89156404879832	2.899870831783611	7.493531504216724	2.715033615201348
7	88.4125379497188	2.2377324483306964	5.411268857873815	3.938460744076697
8	79.51275909280507	4.302729636642788	10.3817037779888	5.802807492563348
9	54.17690962823596	17.249725487285282	14.988676602641938	13.584688281836819
10	45.03605979657418	18.369398508554873	21.681461980896657	14.91307971397429
11	40.1725254927715	17.33016315857839	24.836522736752592	17.660788611897516
12	19.62227373173512	36.19848499639765	26.54322133107	17.63601994079723
13	14.549536938802019	38.91432540870324	29.056152271658263	17.47998538083647
14	13.119166352688127	22.914732034443784	45.32844306715214	18.637658545715947
15	15.311879522602018	16.956777458734347	47.34188592050305	20.389457098160584
16	16.213717325725774	20.739042283429438	25.91416162889108	37.13307876195371
17	20.421406263488638	22.68003475681925	32.7674189511799	24.13114002851221
18	25.680674546922916	24.453213432526347	26.925885197612846	22.940226822937888
19	28.657594502161814	21.466127833773978	24.65499338829773	25.221284275766475
20	21.025535935146422	28.398047873726572	27.897268911122424	22.67914728000458
21	21.806596211764877	23.450606671082536	27.10547823301753	27.63731888413506
22	19.09027172119674	28.67631219498027	23.688047058861496	28.5453690249615
23	19.58265999391675	21.85766646846351	37.92495011976903	20.634723417850708
24	19.296973139304008	21.610544515433624	34.659922918604664	24.432559426657704
25	17.64852529591301	32.152478198325255	26.758152079640556	23.440844426121185
26	18.639675538476556	29.25462435930225	27.256429971221547	24.849270130999646
27	18.935447356892347	25.390146909684713	36.645611951569585	19.02879378185336
28	17.239237124930114	22.412823555893695	34.05095246432141	26.296986854854783
29	21.782553658058408	26.173385538484624	29.47842987601949	22.565630927437475
30	28.00650923903704	24.426427768665455	24.966094351694153	22.600968640603355
31	30.609156017617224	26.295615299777563	22.706013623575902	20.38921505902931
32	27.963345593959993	27.16889248541109	23.571142158456563	21.29661976217235
33	19.77516178298933	25.53972709281152	26.479323000413885	28.205788123785265
34	21.092016016536114	26.176693406612024	29.036466422314717	23.694824154537145
35	29.985985934299286	23.108201980041454	24.914943415285094	21.99086867037417
36	21.90591293529729	27.616100120293446	26.418167779912206	24.059819164497053
37	21.563024165993664	29.727810860941258	25.29397669485885	23.41518827820623
38	20.1714605205939	29.11375758490128	25.18191257707938	25.532869317425444
39	26.011300000242038	24.20270293165864	24.593838167796047	25.192158900303273
40	17.913880863498804	22.38402089927219	29.58040902999591	30.1216892072331
41	23.034380045003143	25.834208035860517	22.664786291549042	28.466625627587298
42	23.280695200928786	23.31409660104448	27.210361856569225	26.194846341457513
43	24.092413767508507	25.61298426987686	26.848916753867986	23.44568520874665
44	19.5040779559634	30.74881259636183	23.41018613615992	26.336923311514848
45	23.378963088225685	27.138960312843647	25.709235164413148	23.772841434517524
46	24.629821318645323	24.211658379515743	26.029049536535403	25.12947076530353
47	23.15927223674009	21.43635702062738	31.020783900202424	24.383586842430105
48	20.75235443564946	20.07819477534331	29.70126723621164	29.46818355279559
49	21.029085842405095	26.4419682944874	29.1042373790712	23.42470848403631
50	19.319321419091562	28.479695740676046	26.44237169303952	25.758611147192873
51	21.417316608967226	22.82816270515842	28.102598774152483	27.65192191172187
52	23.63399165287716	21.629665606804206	30.498221415783693	24.23812132453494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	185.0
1	365.0
2	545.0
3	2533.0
4	4521.0
5	3080.5
6	1640.0
7	1813.5
8	1987.0
9	2101.5
10	2216.0
11	2150.0
12	2084.0
13	2060.5
14	1966.0
15	1895.0
16	1762.0
17	1629.0
18	1862.0
19	2095.0
20	2148.0
21	2201.0
22	2551.0
23	2901.0
24	3339.0
25	3777.0
26	4226.0
27	4675.0
28	5051.5
29	5428.0
30	6937.5
31	8447.0
32	10553.0
33	12659.0
34	15755.0
35	18851.0
36	20902.5
37	22954.0
38	25084.0
39	30283.5
40	33353.0
41	48859.0
42	64365.0
43	82308.5
44	100252.0
45	110145.5
46	120039.0
47	116025.0
48	112011.0
49	117749.5
50	123488.0
51	127884.0
52	132280.0
53	117548.0
54	102816.0
55	88614.0
56	74412.0
57	65836.5
58	57261.0
59	52672.5
60	48084.0
61	45275.0
62	42466.0
63	38176.5
64	29054.0
65	24221.0
66	19813.5
67	15406.0
68	12122.0
69	8838.0
70	7364.0
71	5890.0
72	5527.0
73	5164.0
74	3868.0
75	2572.0
76	2161.5
77	1751.0
78	1112.5
79	474.0
80	353.5
81	233.0
82	190.5
83	148.0
84	128.5
85	109.0
86	56.5
87	4.0
88	3.5
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1239469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.299820143127988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.28177802629	14.54389591012966
2	13.062252284376829	5.564472486427784
3	5.502995361794123	3.5163843436404703
4	2.8035237267678497	2.388582045885884
5	1.6225798213746554	1.728032918157445
6	1.019366076242784	1.3027388450386437
7	0.6539548936964695	0.9750385132217229
8	0.46250134406377025	0.788095635561061
9	0.3666070152311971	0.7027797138870134
>10	3.5316821243197127	19.088524618757155
>50	2.337528091112107	34.91932581809247
>100	0.3434409046698635	11.174964931069649
>500	0.006465664871968542	0.8719947987823589
>1k	0.00494433196091712	1.9854812823631398
>5k	3.8033322776285535E-4	0.44968813898558846
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	5551	0.44785307256575196	RNA PCR Primer, Index 34 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT	4814	0.3883921259829814	RNA PCR Primer, Index 34 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	3804	0.30690561845435427	TruSeq Adapter, Index 18 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2492	0.2010538383775633	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	1759	0.1419156106364903	TruSeq Adapter, Index 18 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1619	0.13062045117707666	TruSeq Adapter, Index 3 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1565	0.12626374681415994	RNA PCR Primer, Index 34 (96% over 25bp)
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT	1431	0.11545266561729256	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1410	0.11375839169838052	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39863844920687813	0.0
2	0.0	0.0	0.0	1.4163323165000496	0.0
3	0.0	0.0	0.0	2.22159650624582	0.0
4	0.0	0.0	0.0	2.7018021426917493	0.0
5	0.0	0.0	0.0	5.174715946909523	0.0
6	8.067971042438335E-5	0.0	0.0	5.517120637950606	0.0
7	8.067971042438335E-5	0.0	0.0	6.728607169683147	0.0
8	8.067971042438335E-5	0.0	0.0	8.261602347456854	0.0
9	8.067971042438335E-5	0.0	0.0	9.125924085233274	0.0
10	8.067971042438335E-5	0.0	0.0	9.991778737507754	0.0
11	8.067971042438335E-5	0.0	0.0	10.84940405931895	0.0
12	1.613594208487667E-4	0.0	0.0	11.602952554682691	0.0
13	1.613594208487667E-4	0.0	0.0	11.925752076090648	0.0
14	1.613594208487667E-4	0.0	0.0	12.177150053773026	0.0
15	1.613594208487667E-4	0.0	0.0	13.009280587090117	0.0
16	1.613594208487667E-4	0.0	0.0	13.912651304711938	0.0
17	2.4203913127315003E-4	0.0	0.0	15.024740433201638	0.0
18	2.4203913127315003E-4	0.0	0.0	15.604827551152953	0.0
19	2.4203913127315003E-4	0.0	0.0	16.28802333902663	0.0
20	3.227188416975334E-4	0.0	0.0	17.16057440726634	0.0
21	4.033985521219167E-4	0.0	0.0	17.87975334598929	0.0
22	4.033985521219167E-4	0.0	0.0	18.722452921371975	0.0
23	4.033985521219167E-4	0.0	0.0	19.32553375679424	0.0
24	4.033985521219167E-4	0.0	0.0	19.822440093298017	0.0
25	4.033985521219167E-4	0.0	0.0	20.33346537912606	0.0
26	4.033985521219167E-4	0.0	0.0	20.77889806037908	0.0
27	4.033985521219167E-4	0.0	0.0	21.289923346207125	0.0
28	4.033985521219167E-4	0.0	0.0	21.7348719491976	0.0
29	4.033985521219167E-4	0.0	0.0	22.354169406415167	0.0
30	4.033985521219167E-4	0.0	0.0	22.931029335949507	0.0
31	4.033985521219167E-4	0.0	0.0	23.411719050657982	0.0
32	4.033985521219167E-4	0.0	0.0	23.870625243551874	0.0
33	4.033985521219167E-4	0.0	0.0	24.317106761040414	0.0
34	4.033985521219167E-4	0.0	0.0	24.98335980972497	0.0
35	4.033985521219167E-4	0.0	0.0	25.457917866441193	0.0
36	4.033985521219167E-4	0.0	0.0	25.93715534636203	0.0
37	4.033985521219167E-4	0.0	0.0	26.38734813053009	0.0
38	4.8407826254630006E-4	0.0	0.0	26.82374468421558	0.0
39	4.8407826254630006E-4	0.0	0.0	27.329848507707737	0.0
40	4.8407826254630006E-4	0.0	0.0	27.842003309481722	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	6.311646E-4	46.000004	20
ACCGGTA	35	1.0196527E-7	46.000004	41
GTATTCG	35	1.0196527E-7	46.000004	11
CGTGATA	20	6.311646E-4	46.000004	30
CCGGATA	20	6.311646E-4	46.000004	10
ATCGTAC	40	5.6115823E-9	46.000004	33
GTTACGA	20	6.311646E-4	46.000004	35
CTAACGG	140	0.0	46.000004	1
CCGTAGG	75	0.0	46.000004	1
CCGTAAG	20	6.311646E-4	46.000004	22
CGCTAAT	20	6.311646E-4	46.000004	27
TATTCGA	35	1.0196527E-7	46.000004	12
ACAGCGA	20	6.311646E-4	46.000004	23
CTCGCTA	20	6.311646E-4	46.000004	25
CGTACTC	20	6.311646E-4	46.000004	31
CGTACCC	20	6.311646E-4	46.000004	19
CGTACAA	40	5.6115823E-9	46.000004	35
CCGCGTA	20	6.311646E-4	46.000004	19
CGGTCGT	20	6.311646E-4	46.000004	27
TAAATCG	20	6.311646E-4	46.000004	10
>>END_MODULE