FastQCFastQC Report
Thu 26 May 2016
SRR1544859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544859_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences879257
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT69450.789871448279627RNA PCR Primer, Index 34 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT51060.5807175831412204RNA PCR Primer, Index 34 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT40450.46004751739252575TruSeq Adapter, Index 18 (95% over 24bp)
TGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTTG25240.28706055226173915TruSeq Adapter, Index 18 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA20100.22860210382174953No Hit
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT16250.1848151336867378TruSeq Adapter, Index 18 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC15140.1721908384010591TruSeq Adapter, Index 3 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC14650.16661795129296667RNA PCR Primer, Index 34 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC13700.15581337424666508TruSeq Adapter, Index 18 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.12840386826604736No Hit
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT10720.1219211220382664TruSeq Adapter, Index 3 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC10670.12135246008846107RNA PCR Primer, Index 34 (96% over 25bp)
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT9910.11270879845141978No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9610.1092968267525877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA700.046.00000441
AACCGTA206.310641E-446.020
AATCCGG501.6370905E-1146.01
GTACCGG206.310641E-446.01
ACGTTAT206.310641E-446.010
GACCGGA206.310641E-446.021
TTCACGG206.310641E-446.01
ACCGACA253.4164965E-546.030
GATCATC253.4164965E-546.010
CACGACG551.8189894E-1246.025
AATGCGA206.310641E-446.019
CTAACGG1150.046.01
CGAGGTA253.4164965E-546.03
ACTACGG551.8189894E-1246.01
TCGTCCG206.310641E-446.014
CAATGCG206.310641E-446.018
CGATGCG301.8609007E-646.09
CACACGG900.046.01
CGCGTAG206.310641E-446.046
ACGAGTA206.310641E-446.031
Produced by FastQC (version 0.11.5)