FastQCFastQC Report
Thu 26 May 2016
SRR1544858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544858_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1088256
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT42310.388787197130087Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT35100.3225344036697248Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT33640.3091184427193602Illumina Single End Adapter 1 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA20690.19012070689249588No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20560.18892613502705247No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17270.15869427781698425No Hit
ACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC12130.11146274406022112Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT11760.10806280875088214Illumina Single End Adapter 1 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC11010.10117104798870855Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC10950.10061970712773466Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC850.046.00000424
GTATTCG206.3113053E-446.08
CGAAAAG206.3113053E-446.030
ACGTTAC301.8613118E-646.012
TGGACCG206.3113053E-446.046
ATGTCGT206.3113053E-446.021
GTTACGA253.417036E-546.016
ACGATAG206.3113053E-446.038
TACGATC206.3113053E-446.028
AAGCACG301.8613118E-646.08
TCGCTAA253.417036E-546.016
GGTATTC206.3113053E-446.07
CTACCGA206.3113053E-446.027
CGTAATG253.417036E-546.034
ACGTAGG1350.046.01
CCGTTGG206.3113053E-446.01
CCGTTAT253.417036E-546.024
TGCCGTA206.3113053E-446.044
CTTACGT301.8613118E-646.022
GTCGTAG351.0195072E-745.9999961
Produced by FastQC (version 0.11.5)