##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544858_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1088256
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.898285881263234	34.0	33.0	34.0	31.0	34.0
2	33.23149056839567	34.0	33.0	34.0	31.0	34.0
3	33.28850288902611	34.0	34.0	34.0	31.0	34.0
4	36.56928333039285	37.0	37.0	37.0	35.0	37.0
5	36.56724704481299	37.0	37.0	37.0	35.0	37.0
6	36.66251782668784	37.0	37.0	37.0	35.0	37.0
7	36.63506105181134	37.0	37.0	37.0	35.0	37.0
8	36.525636431133854	37.0	37.0	37.0	35.0	37.0
9	38.314596014173134	39.0	39.0	39.0	37.0	39.0
10	38.12067473094566	39.0	39.0	39.0	35.0	39.0
11	37.440368810279935	39.0	35.0	39.0	35.0	39.0
12	37.33087894760057	39.0	35.0	39.0	35.0	39.0
13	37.310965434603624	39.0	35.0	39.0	35.0	39.0
14	38.60083473006352	41.0	35.0	41.0	35.0	41.0
15	38.67822828452129	41.0	35.0	41.0	35.0	41.0
16	38.67123820130558	41.0	35.0	41.0	35.0	41.0
17	38.722943866149144	41.0	36.0	41.0	35.0	41.0
18	38.711014687720535	41.0	36.0	41.0	35.0	41.0
19	38.63417798753235	41.0	35.0	41.0	35.0	41.0
20	38.540364583333336	41.0	35.0	41.0	35.0	41.0
21	38.47744648318043	40.0	35.0	41.0	35.0	41.0
22	38.44034491884263	40.0	35.0	41.0	35.0	41.0
23	38.40335729828276	40.0	35.0	41.0	35.0	41.0
24	38.347876786344386	40.0	35.0	41.0	35.0	41.0
25	38.30572034521289	40.0	35.0	41.0	35.0	41.0
26	38.238510975358736	40.0	35.0	41.0	35.0	41.0
27	38.20944520406963	40.0	35.0	41.0	35.0	41.0
28	38.242411711950126	40.0	35.0	41.0	35.0	41.0
29	38.231429920900965	40.0	36.0	41.0	35.0	41.0
30	38.17980879498941	40.0	35.0	41.0	35.0	41.0
31	38.1155812602917	40.0	35.0	41.0	35.0	41.0
32	38.04883134115502	40.0	35.0	41.0	35.0	41.0
33	37.97149016407904	40.0	35.0	41.0	35.0	41.0
34	37.8778127572924	40.0	35.0	41.0	35.0	41.0
35	37.818910256410255	40.0	35.0	41.0	35.0	41.0
36	37.712075099976474	40.0	35.0	41.0	35.0	41.0
37	37.658697953422724	40.0	35.0	41.0	35.0	41.0
38	37.61982107151258	40.0	35.0	41.0	35.0	41.0
39	37.50353593272171	40.0	35.0	41.0	34.0	41.0
40	37.420662969007296	39.0	35.0	41.0	34.0	41.0
41	37.38530823629734	39.0	35.0	41.0	34.0	41.0
42	37.30424642731122	39.0	35.0	41.0	34.0	41.0
43	37.28562305192896	39.0	35.0	41.0	34.0	41.0
44	37.272755675135265	39.0	35.0	41.0	34.0	41.0
45	37.2543399714773	39.0	35.0	41.0	34.0	41.0
46	37.180899530992704	39.0	35.0	41.0	34.0	41.0
47	37.101273046047986	38.0	35.0	41.0	34.0	41.0
48	37.0764608695013	38.0	35.0	41.0	34.0	41.0
49	37.016916056516116	38.0	35.0	41.0	34.0	41.0
50	36.92830271553752	38.0	35.0	41.0	34.0	41.0
51	36.86142231239708	37.0	35.0	41.0	34.0	41.0
52	36.610252550870385	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	4.0
15	8.0
16	13.0
17	43.0
18	74.0
19	171.0
20	282.0
21	465.0
22	819.0
23	1286.0
24	2130.0
25	3368.0
26	4844.0
27	5798.0
28	5909.0
29	6481.0
30	7559.0
31	9622.0
32	12342.0
33	17813.0
34	56324.0
35	221023.0
36	42538.0
37	66387.0
38	113107.0
39	505597.0
40	4246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.40100491060927	21.604015966831334	23.873794401317337	20.12118472124206
2	27.255443572100685	24.35327717007763	25.708197335920957	22.68308192190073
3	27.421121500823336	24.251003440366972	25.619155786873677	22.708719271936015
4	26.951746647847564	26.57242413549753	22.65349329569513	23.822335920959777
5	25.357636438485066	35.344257233592096	20.267841390261115	19.030264937661727
6	86.66324835332863	3.286910432839332	7.261894260174076	2.7879469536579626
7	87.72439573041638	3.0519473359209597	5.402313426252647	3.821343507410021
8	78.01482371794873	5.5764452481769	10.068678693248648	6.340052340625735
9	50.86606460244648	21.333491384380146	13.834520553987298	13.965923459186074
10	42.4260468125147	21.096782374735355	22.67720095271701	13.799969860032935
11	40.930350946836036	18.747335185838626	23.04457774641261	17.277736120912728
12	21.76987767584098	33.94624059044931	26.239506145612797	18.044375588096916
13	15.515375058809692	36.123026199717714	27.65351167372383	20.708087067748764
14	14.13895259938838	25.3693983768525	41.84787402964009	18.64377499411903
15	16.526074747118326	18.310397553516818	45.27133321571395	19.892194483650904
16	17.253477123029874	21.508174547165375	26.879980445777463	34.35836788402729
17	20.211053281580803	23.70756513173371	30.991237355916258	25.09014423076923
18	25.721153846153843	27.299734621265586	25.35938235121148	21.61972918136909
19	30.216695336391435	22.354666549047284	24.179421018583863	23.249217095977418
20	22.38407139496589	28.98931868972007	26.647682163020463	21.97892775229358
21	21.692689955304633	24.43809177252411	25.597653493295695	28.271564778875558
22	20.409535991531406	29.106386732533522	23.362425752764054	27.12165152317102
23	20.65690425782169	22.12310338743825	36.120361385556336	21.09963096918372
24	20.859246353799108	21.580308309809457	32.51431648435662	25.046128852034816
25	17.796088420371678	31.74133659727123	26.35170401082098	24.11087097153611
26	18.048510644554224	29.861999382498233	27.260773200423426	24.82871677252411
27	19.798282756998354	26.84625676311456	33.70603975535168	19.649420724535403
28	17.577389878852035	26.22801987767584	30.627903728534463	25.566686514937665
29	18.890132468830863	28.680659697718188	27.12266231474947	25.306545518701483
30	25.36259850623383	28.972410903316863	21.59758365678664	24.067406933662667
31	26.499371471418492	29.6917269466008	22.003278640319927	21.805622941660786
32	24.43809177252411	30.243343478005176	22.89167254763585	22.426892201834864
33	18.2474528052223	28.253921871324394	25.333377440602213	28.165247882851098
34	18.769940161138557	29.177601593742647	27.68769480710421	24.364763438014585
35	27.592956069160195	26.026229122559396	23.032907698188662	23.347907110091743
36	19.94190705128205	30.40644848270995	27.106305869207247	22.545338596800754
37	21.472704951776052	30.112859474241354	25.05302061279699	23.361414961185602
38	20.536620059985886	29.32775008821454	25.156764584803575	24.978865266996
39	25.625955657492355	24.043974947071277	26.878142642907548	23.451926752528816
40	16.861198100446952	22.898288637967536	31.18705525170548	29.05345800988003
41	21.42694366031522	27.00504293107504	23.442462067748764	28.125551340860973
42	22.19128587391202	25.80514143730887	26.09073600329334	25.912836685485768
43	21.56624911785462	28.942730386967774	26.493122941660786	22.997897553516818
44	18.459627146553753	31.237962391202068	24.158653846153847	26.14375661609033
45	21.714192248882615	29.193958039284873	23.958057662902846	25.133792048929664
46	22.67674150199953	26.322850505763352	26.13098388614444	24.869424106092684
47	21.952922841684313	22.28234900611621	31.374970595154082	24.389757557045403
48	19.710068219242533	22.567392231239708	29.22317910491649	28.49936044460127
49	19.71741943072218	26.670103358033405	29.59073967889908	24.02173753234533
50	18.124871353799108	28.076941454951776	27.461645054104917	26.336542137144203
51	20.21417754645966	23.862216243236887	28.423918636791345	27.499687573512116
52	22.404287226534933	21.674495706892497	30.55099167842861	25.370225388143968
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	198.0
1	390.0
2	582.0
3	2207.5
4	3833.0
5	2672.0
6	1511.0
7	1547.5
8	1584.0
9	1624.0
10	1664.0
11	1581.0
12	1498.0
13	1495.0
14	1529.0
15	1566.0
16	1509.0
17	1452.0
18	1512.5
19	1573.0
20	1865.5
21	2158.0
22	2059.5
23	1961.0
24	2304.0
25	2647.0
26	3769.0
27	4891.0
28	6796.5
29	8702.0
30	9169.5
31	9637.0
32	11030.5
33	12424.0
34	15297.5
35	18171.0
36	19144.5
37	20118.0
38	22902.0
39	30412.0
40	35138.0
41	49092.0
42	63046.0
43	83332.0
44	103618.0
45	118134.5
46	132651.0
47	129262.5
48	125874.0
49	110930.5
50	95987.0
51	85762.5
52	75538.0
53	67366.5
54	59195.0
55	53611.0
56	48027.0
57	43977.5
58	39928.0
59	40934.0
60	41940.0
61	41940.0
62	41940.0
63	40020.0
64	30910.5
65	23721.0
66	18908.0
67	14095.0
68	11722.5
69	9350.0
70	7621.0
71	5892.0
72	5021.5
73	4151.0
74	3481.0
75	2811.0
76	2694.0
77	2577.0
78	1659.0
79	741.0
80	521.0
81	301.0
82	221.0
83	141.0
84	99.0
85	57.0
86	66.5
87	76.0
88	41.5
89	5.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1088256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.226188071323666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73593430443592	15.499639911798646
2	12.328817586760769	5.480452367607752
3	4.769186696026879	3.180025213594445
4	2.3383171853783336	2.078875101305082
5	1.3065926257059086	1.4520286715772066
6	0.8549930962948782	1.1401942414759985
7	0.5762976713858814	0.8966230300501938
8	0.43407366237847855	0.7718242285465854
9	0.33337596437931494	0.6668709194478198
>10	5.315715870800779	32.7828974853051
>50	1.7761741376371696	25.02845422899152
>100	0.21932682405311643	7.873860512357774
>500	0.006219097090353018	0.971458101951848
>1k	0.004975277672282414	2.1767959859899975
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	4231	0.388787197130087	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT	3510	0.3225344036697248	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	3364	0.3091184427193602	Illumina Single End Adapter 1 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2069	0.19012070689249588	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2056	0.18892613502705247	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1727	0.15869427781698425	No Hit
ACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1213	0.11146274406022112	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	1176	0.10806280875088214	Illumina Single End Adapter 1 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1101	0.10117104798870855	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1095	0.10061970712773466	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33732871677252413	0.0
2	0.0	0.0	0.0	1.2573328334509528	0.0
3	0.0	0.0	0.0	1.9855622206539638	0.0
4	0.0	0.0	0.0	2.454477622912256	0.0
5	0.0	0.0	0.0	4.801535668078099	0.0
6	0.0	0.0	0.0	5.175069101387908	0.0
7	0.0	0.0	0.0	6.50444380733945	0.0
8	0.0	0.0	0.0	8.096440543401553	0.0
9	0.0	0.0	0.0	9.234959421312633	0.0
10	0.0	0.0	0.0	10.11379675370501	0.0
11	0.0	0.0	0.0	10.937775670430486	0.0
12	0.0	0.0	0.0	11.681075041166784	0.0
13	0.0	0.0	0.0	12.000485179957657	0.0
14	0.0	0.0	0.0	12.249875029404846	0.0
15	0.0	0.0	0.0	13.087269907080687	0.0
16	0.0	0.0	0.0	13.944421165608093	0.0
17	0.0	0.0	0.0	15.056291901905434	0.0
18	0.0	0.0	0.0	15.567844330745707	0.0
19	0.0	0.0	0.0	16.174962508821455	0.0
20	0.0	0.0	0.0	16.93241296165608	0.0
21	0.0	0.0	0.0	17.601465096447896	0.0
22	0.0	0.0	0.0	18.34899141378499	0.0
23	0.0	0.0	0.0	18.890591919548342	0.0
24	0.0	0.0	0.0	19.36511262055987	0.0
25	9.189014349564808E-5	0.0	0.0	19.806093419195484	0.0
26	9.189014349564808E-5	0.0	0.0	20.215923459186072	0.0
27	9.189014349564808E-5	0.0	0.0	20.646336891319688	0.0
28	9.189014349564808E-5	0.0	0.0	21.067653199247236	0.0
29	9.189014349564808E-5	0.0	0.0	21.596848535638674	0.0
30	9.189014349564808E-5	0.0	0.0	22.126778993178075	0.0
31	9.189014349564808E-5	0.0	0.0	22.60010512232416	0.0
32	1.8378028699129616E-4	0.0	0.0	23.03989134909433	0.0
33	1.8378028699129616E-4	0.0	0.0	23.469293989649493	0.0
34	1.8378028699129616E-4	0.0	0.0	24.06419077864032	0.0
35	1.8378028699129616E-4	0.0	0.0	24.50783639143731	0.0
36	1.8378028699129616E-4	0.0	0.0	24.99108665608092	0.0
37	1.8378028699129616E-4	0.0	0.0	25.428391848976712	0.0
38	1.8378028699129616E-4	0.0	0.0	25.8382218889673	0.0
39	1.8378028699129616E-4	0.0	0.0	26.31531551399671	0.0
40	2.7567043048694426E-4	0.0	0.0	26.824386908962598	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	85	0.0	46.000004	24
GTATTCG	20	6.3113053E-4	46.0	8
CGAAAAG	20	6.3113053E-4	46.0	30
ACGTTAC	30	1.8613118E-6	46.0	12
TGGACCG	20	6.3113053E-4	46.0	46
ATGTCGT	20	6.3113053E-4	46.0	21
GTTACGA	25	3.417036E-5	46.0	16
ACGATAG	20	6.3113053E-4	46.0	38
TACGATC	20	6.3113053E-4	46.0	28
AAGCACG	30	1.8613118E-6	46.0	8
TCGCTAA	25	3.417036E-5	46.0	16
GGTATTC	20	6.3113053E-4	46.0	7
CTACCGA	20	6.3113053E-4	46.0	27
CGTAATG	25	3.417036E-5	46.0	34
ACGTAGG	135	0.0	46.0	1
CCGTTGG	20	6.3113053E-4	46.0	1
CCGTTAT	25	3.417036E-5	46.0	24
TGCCGTA	20	6.3113053E-4	46.0	44
CTTACGT	30	1.8613118E-6	46.0	22
GTCGTAG	35	1.0195072E-7	45.999996	1
>>END_MODULE