FastQCFastQC Report
Thu 26 May 2016
SRR1544857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544857_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences781971
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT56670.7247071822356583Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT44410.5679238744147801Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT32470.4152327899627991Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA31370.4011657721322146No Hit
TGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTTG22050.2819797665130804Illumina Single End Adapter 1 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC12940.16547928247978508Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC12140.15524872405754178Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11780.1506449727675323No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA11460.146552749398635No Hit
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT11340.1450181656352985Illumina Single End Adapter 1 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC10890.13926347652278664Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.11995329750080246No Hit
CTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTTGAAA9060.11586107413190515Illumina Single End Adapter 1 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC8980.11483801828968082Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT8750.11189673274328588Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCG351.01901605E-746.00000438
GTCATCG206.3102104E-446.043
CTTCGAA253.4161472E-546.019
GGTCGTC206.3102104E-446.046
TCCGATG206.3102104E-446.039
AACCCGC253.4161472E-546.021
GTCGTAG405.6079443E-946.01
GTCGTAA206.3102104E-446.032
GCGATAT453.092282E-1046.08
TTACGAG206.3102104E-446.023
ACGTAGG600.046.01
CTTTACG253.4161472E-546.021
CACAACG253.4161472E-546.011
ATACCGG301.8606333E-646.01
CATGCGG1850.044.7567561
TAGTAGG1450.044.413791
GACCGAT950.043.578958
GGCGATA1850.043.5135157
AATAAGG800.043.1251
ATCGAGG750.042.933331
Produced by FastQC (version 0.11.5)