##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544857_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	781971
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.884817979183374	34.0	33.0	34.0	31.0	34.0
2	33.24716773384179	34.0	33.0	34.0	31.0	34.0
3	33.34052285826456	34.0	34.0	34.0	31.0	34.0
4	36.57418497616919	37.0	37.0	37.0	35.0	37.0
5	36.58002150974908	37.0	37.0	37.0	35.0	37.0
6	36.4971475924299	37.0	37.0	37.0	35.0	37.0
7	36.37170432151576	37.0	37.0	37.0	35.0	37.0
8	36.40753813120947	37.0	37.0	37.0	35.0	37.0
9	38.257426426299695	39.0	39.0	39.0	37.0	39.0
10	38.13504464999341	39.0	39.0	39.0	35.0	39.0
11	37.54397797360772	39.0	35.0	39.0	35.0	39.0
12	37.48579423022081	39.0	35.0	39.0	35.0	39.0
13	37.469738647596905	39.0	35.0	39.0	35.0	39.0
14	38.742342107316	41.0	35.0	41.0	35.0	41.0
15	38.80799287953134	41.0	35.0	41.0	35.0	41.0
16	38.813542957475406	41.0	35.0	41.0	35.0	41.0
17	38.874625785355214	41.0	36.0	41.0	35.0	41.0
18	38.877304912842035	41.0	36.0	41.0	35.0	41.0
19	38.80887654401506	41.0	36.0	41.0	35.0	41.0
20	38.732097481875925	41.0	35.0	41.0	35.0	41.0
21	38.66894936001463	41.0	35.0	41.0	35.0	41.0
22	38.64385507902467	41.0	35.0	41.0	35.0	41.0
23	38.61460207603607	41.0	35.0	41.0	35.0	41.0
24	38.5481072827509	40.0	35.0	41.0	35.0	41.0
25	38.48488626816084	40.0	35.0	41.0	35.0	41.0
26	38.42456561688349	40.0	35.0	41.0	35.0	41.0
27	38.41341047174384	40.0	35.0	41.0	35.0	41.0
28	38.438100645676116	40.0	36.0	41.0	35.0	41.0
29	38.484982179646046	40.0	36.0	41.0	35.0	41.0
30	38.44046901995087	40.0	35.0	41.0	35.0	41.0
31	38.4023525169092	40.0	35.0	41.0	35.0	41.0
32	38.36538183641081	40.0	35.0	41.0	35.0	41.0
33	38.28662955531599	40.0	35.0	41.0	35.0	41.0
34	38.22409526696003	40.0	35.0	41.0	35.0	41.0
35	38.159150403275824	40.0	35.0	41.0	35.0	41.0
36	38.07104739178307	40.0	35.0	41.0	35.0	41.0
37	38.04848645282242	40.0	35.0	41.0	35.0	41.0
38	37.96971115297114	40.0	35.0	41.0	35.0	41.0
39	37.918870392891804	40.0	35.0	41.0	35.0	41.0
40	37.8732408235088	40.0	35.0	41.0	35.0	41.0
41	37.84282281567987	40.0	35.0	41.0	35.0	41.0
42	37.78810211631889	40.0	35.0	41.0	35.0	41.0
43	37.70870275240386	40.0	35.0	41.0	35.0	41.0
44	37.599950637555615	39.0	35.0	41.0	35.0	41.0
45	37.59107179166491	39.0	35.0	41.0	35.0	41.0
46	37.5190409874535	39.0	35.0	41.0	35.0	41.0
47	37.44919184982563	39.0	35.0	41.0	35.0	41.0
48	37.3927869447844	39.0	35.0	41.0	35.0	41.0
49	37.354690902859566	39.0	35.0	41.0	35.0	41.0
50	37.27666115495332	39.0	35.0	41.0	35.0	41.0
51	37.205996130291275	39.0	35.0	41.0	34.0	41.0
52	36.7943146229208	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	0.0
13	5.0
14	13.0
15	13.0
16	28.0
17	26.0
18	57.0
19	106.0
20	163.0
21	283.0
22	506.0
23	730.0
24	1158.0
25	1907.0
26	2853.0
27	3271.0
28	3434.0
29	3581.0
30	4400.0
31	5699.0
32	7311.0
33	10916.0
34	35420.0
35	150576.0
36	29277.0
37	46633.0
38	82314.0
39	388888.0
40	2400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15723984649047	21.347978377714774	22.89944256244797	22.595339213346787
2	26.954068629143535	24.633266451057647	25.619108637021064	22.79355628277775
3	27.640794863236618	24.077491364769283	25.51437329517335	22.767340476820753
4	26.99690909253668	25.874488951636316	23.31531476231216	23.81328719351485
5	25.11001047353418	34.417005234209455	20.470452229046856	20.002532063209504
6	84.26092527728011	3.3231411395051738	8.558245766147337	3.8576878170673847
7	85.57683085434114	3.056251446664902	6.418524472135156	4.948393226858797
8	74.42219724260875	5.759037099841299	11.937143449053737	7.881622208496224
9	49.111795705978864	20.492320047674404	14.916665707551816	15.479218538794917
10	40.26760583192983	20.630048940433852	24.810255111762455	14.292090115873862
11	38.31587104892636	18.512195465049217	24.05012462098978	19.121808865034637
12	21.069195660708644	32.15413359318951	27.754865589644627	19.02180515645721
13	15.272689140645882	34.431200134020315	28.838921136461583	21.45718958887222
14	13.864452773824093	25.27676857581675	40.51863815921563	20.340140491143533
15	15.837160201593154	18.402344843990377	44.67441887231112	21.086076082105347
16	16.503681082802306	22.682810487856965	26.907903234263163	33.90560519507756
17	19.06490138381091	23.944877751220954	31.277502618383547	25.712718246584593
18	24.582625186867542	27.320066856699288	25.981270405168477	22.11603755126469
19	28.628171632963372	22.14557828870892	24.675851150490235	24.550398927837477
20	21.37304324584927	29.496618161031545	26.980923845001925	22.14941474811726
21	20.961519033314534	24.323280530863677	25.86195651756907	28.853243918252723
22	19.657122834478518	29.12512100832384	23.510462664216448	27.7072934929812
23	19.583590695818643	22.35875754983241	36.14916665707552	21.908485097273427
24	21.144901793033245	21.737890535582522	31.631863585734	25.485344085650237
25	17.64310952707965	31.11752226105572	26.182556642126116	25.056811569738517
26	17.783268177464382	29.12345854258022	26.584617588120274	26.50865569183512
27	20.61534251270188	26.819153139950203	32.258613171076675	20.306891176271243
28	17.375836188298543	26.82055984173326	29.395079868690782	26.408524101277415
29	18.86297573695188	28.698378840136012	25.679213167751747	26.75943225516036
30	24.55410750526554	29.772203828530724	20.725321015740995	24.948367650462743
31	25.823719805465934	29.70979742215504	21.11459376370735	23.351889008671677
32	23.63041596171725	30.494992781062212	22.562345662435053	23.312245594785487
33	17.616382193201538	27.8470684974251	25.155280694552612	29.381268614820755
34	18.7102846524999	29.339579089250112	27.467003252038758	24.483133006211226
35	27.31776498105428	25.871036138168808	22.823480666162812	23.987718214614098
36	19.97490955546945	30.35892635404638	26.47156991755449	23.194594172929687
37	21.46179334016223	30.275803066865652	25.29416052513456	22.968243067837555
38	20.321086076082107	29.092766867313497	24.53927319555329	26.04687386105111
39	26.183323934007785	23.264417734161498	26.81454938866019	23.737708943170528
40	16.99935163835999	22.044551524289265	31.182358425056684	29.77373841229406
41	21.067916840905866	27.05432810168152	23.329253898162463	28.54850115925015
42	21.520619051090133	24.780330728377393	26.76186201278564	26.937188207746836
43	22.228573693909365	27.745402323104052	26.365299991943438	23.660723991043145
44	18.36653788951253	29.57360311315893	25.02292284496484	27.036936152363705
45	21.222398273081737	27.874051595263765	24.25000415616436	26.65354597549014
46	23.00622401598013	24.719203141804492	26.434100497333024	25.840472344882354
47	20.821104618969247	21.667299682469043	32.48087205279992	25.0307236457618
48	19.38908220381574	21.361533867624246	29.117320207526877	30.13206372103313
49	19.691139441232476	25.168708302481807	30.137051118263976	25.003101138021744
50	18.496465981475016	26.194193902331413	28.271508789967914	27.037831326225653
51	20.11506820585418	22.303768298312853	28.65323650109787	28.9279269947351
52	21.878688595868645	20.806653955197827	32.190068429647646	25.12458901928588
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134.0
1	214.0
2	294.0
3	1094.5
4	1895.0
5	1375.5
6	856.0
7	884.5
8	913.0
9	948.0
10	983.0
11	1004.5
12	1026.0
13	944.5
14	927.0
15	991.0
16	939.0
17	887.0
18	952.5
19	1018.0
20	1193.5
21	1369.0
22	1334.0
23	1299.0
24	1511.5
25	1724.0
26	2367.5
27	3011.0
28	4030.0
29	5049.0
30	5422.0
31	5795.0
32	6786.5
33	7778.0
34	9565.0
35	11352.0
36	12164.5
37	12977.0
38	14964.5
39	19913.5
40	22875.0
41	32674.5
42	42474.0
43	57871.5
44	73269.0
45	87574.0
46	101879.0
47	100727.5
48	99576.0
49	86954.5
50	74333.0
51	65403.0
52	56473.0
53	49830.5
54	43188.0
55	38580.0
56	33972.0
57	30854.0
58	27736.0
59	28193.0
60	28650.0
61	28308.5
62	27967.0
63	27841.5
64	22015.5
65	16315.0
66	13121.0
67	9927.0
68	8296.5
69	6666.0
70	5485.5
71	4305.0
72	3613.5
73	2922.0
74	2447.5
75	1973.0
76	1846.5
77	1720.0
78	1093.5
79	467.0
80	340.0
81	213.0
82	164.0
83	115.0
84	69.5
85	24.0
86	35.0
87	46.0
88	24.5
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	781971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.962102742505287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.19914226431682	18.225173439019386
2	12.123622051578728	6.295094426287793
3	4.464348785212713	3.477116455202134
4	2.0784800433814015	2.158468497380592
5	1.1174802909069728	1.450606906262576
6	0.787915290708089	1.2273562637852597
7	0.5607829367745184	1.0191372954548727
8	0.44555740371392133	0.9254085674323786
9	0.3450357248504458	0.8062067644561852
>10	7.353411496728035	47.73207236770958
>50	0.39267323320057845	6.787641506558091
>100	0.11770305982487725	5.12889676760022
>500	0.008407361416062662	1.4603732998218821
>1k	0.004945506715330976	2.5788287081873134
>5k	4.945506715330978E-4	0.7276187348417976
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	5667	0.7247071822356583	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTT	4441	0.5679238744147801	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	3247	0.4152327899627991	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3137	0.4011657721322146	No Hit
TGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTTG	2205	0.2819797665130804	Illumina Single End Adapter 1 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1294	0.16547928247978508	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1214	0.15524872405754178	Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1178	0.1506449727675323	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1146	0.146552749398635	No Hit
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	1134	0.1450181656352985	Illumina Single End Adapter 1 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1089	0.13926347652278664	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.11995329750080246	No Hit
CTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCTTGAAA	906	0.11586107413190515	Illumina Single End Adapter 1 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	898	0.11483801828968082	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	875	0.11189673274328588	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5858273516537058	0.0
2	0.0	0.0	0.0	2.027185151367506	0.0
3	0.0	0.0	0.0	3.0709578743968766	0.0
4	0.0	0.0	0.0	3.7217492720318273	0.0
5	0.0	0.0	0.0	6.927238989681203	0.0
6	0.0	0.0	0.0	7.433140103661133	0.0
7	0.0	0.0	0.0	9.220290778046756	0.0
8	0.0	0.0	0.0	11.313846677178566	0.0
9	0.0	0.0	0.0	12.83513071456614	0.0
10	1.27881980278041E-4	0.0	0.0	14.013435280848011	0.0
11	1.27881980278041E-4	0.0	0.0	15.078308530623259	0.0
12	1.27881980278041E-4	0.0	0.0	16.084100305510052	0.0
13	2.55763960556082E-4	0.0	0.0	16.501123443196743	0.0
14	2.55763960556082E-4	0.0	0.0	16.824536971319908	0.0
15	3.83645940834123E-4	0.0	0.0	17.83902472086561	0.0
16	3.83645940834123E-4	0.0	0.0	18.90402585262113	0.0
17	3.83645940834123E-4	0.0	0.0	20.188728226494334	0.0
18	3.83645940834123E-4	0.0	0.0	20.81637298569896	0.0
19	3.83645940834123E-4	0.0	0.0	21.510900020589	0.0
20	3.83645940834123E-4	0.0	0.0	22.40786423025918	0.0
21	3.83645940834123E-4	0.0	0.0	23.198686396298584	0.0
22	3.83645940834123E-4	0.0	0.0	24.051275558812282	0.0
23	5.11527921112164E-4	0.0	0.0	24.683012541385807	0.0
24	5.11527921112164E-4	0.0	0.0	25.209630536170778	0.0
25	5.11527921112164E-4	0.0	0.0	25.72831984817851	0.0
26	5.11527921112164E-4	0.0	0.0	26.206726336398663	0.0
27	5.11527921112164E-4	0.0	0.0	26.679250253526025	0.0
28	5.11527921112164E-4	0.0	0.0	27.153692400357556	0.0
29	5.11527921112164E-4	0.0	0.0	27.744762913202663	0.0
30	5.11527921112164E-4	0.0	0.0	28.3193366505919	0.0
31	5.11527921112164E-4	0.0	0.0	28.829457869921008	0.0
32	5.11527921112164E-4	0.0	0.0	29.321675612011187	0.0
33	6.394099013902051E-4	0.0	0.0	29.769134661004053	0.0
34	6.394099013902051E-4	0.0	0.0	30.41097432001954	0.0
35	6.394099013902051E-4	0.0	0.0	30.87838295793578	0.0
36	6.394099013902051E-4	0.0	0.0	31.37980820260598	0.0
37	6.394099013902051E-4	0.0	0.0	31.81908280486105	0.0
38	6.394099013902051E-4	0.0	0.0	32.26296115840613	0.0
39	6.394099013902051E-4	0.0	0.0	32.76259605535244	0.0
40	6.394099013902051E-4	0.0	0.0	33.267474113490145	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCG	35	1.01901605E-7	46.000004	38
GTCATCG	20	6.3102104E-4	46.0	43
CTTCGAA	25	3.4161472E-5	46.0	19
GGTCGTC	20	6.3102104E-4	46.0	46
TCCGATG	20	6.3102104E-4	46.0	39
AACCCGC	25	3.4161472E-5	46.0	21
GTCGTAG	40	5.6079443E-9	46.0	1
GTCGTAA	20	6.3102104E-4	46.0	32
GCGATAT	45	3.092282E-10	46.0	8
TTACGAG	20	6.3102104E-4	46.0	23
ACGTAGG	60	0.0	46.0	1
CTTTACG	25	3.4161472E-5	46.0	21
CACAACG	25	3.4161472E-5	46.0	11
ATACCGG	30	1.8606333E-6	46.0	1
CATGCGG	185	0.0	44.756756	1
TAGTAGG	145	0.0	44.41379	1
GACCGAT	95	0.0	43.57895	8
GGCGATA	185	0.0	43.513515	7
AATAAGG	80	0.0	43.125	1
ATCGAGG	75	0.0	42.93333	1
>>END_MODULE