FastQCFastQC Report
Thu 26 May 2016
SRR1544856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544856_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1448413
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT52830.3647440336423382No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT44190.30509253921360824Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT41750.28824651532401324No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC32250.22265748788501621No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27000.18641092008978102No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA18160.1253786040307564No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT16400.11322737368416329No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC15350.10597806012511625No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC15250.1052876493099689No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT15230.10514956714693943No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA15070.1040449098427037No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG206.312002E-446.025
TACCCGC206.312002E-446.021
CAAACGT206.312002E-446.012
TATGCGC206.312002E-446.033
TGCGCAC253.4175995E-546.020
GAACGAT301.8617411E-646.08
CGTAAGT206.312002E-446.024
CGGTATG206.312002E-446.05
TCTACGG800.043.1251
ACTACGG1300.042.461541
CGGGCAC1700.041.9411775
CACTAGG2000.041.41
CCAACGG508.731149E-1041.41
TTAATCG2400.041.20833219
ATAAGCG950.041.15789432
CGAATAT3100.040.8064514
GCGTAGG2600.040.692311
CGTAGGG5600.040.6607172
ATGGGCG2100.040.5238084
CGTTTTT19150.040.355091
Produced by FastQC (version 0.11.5)