##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544856_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1448413
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82994422171024	33.0	31.0	34.0	31.0	34.0
2	33.17284434757214	34.0	33.0	34.0	31.0	34.0
3	33.25205863244807	34.0	33.0	34.0	31.0	34.0
4	36.558511280967515	37.0	37.0	37.0	35.0	37.0
5	36.55436467361174	37.0	37.0	37.0	35.0	37.0
6	36.64604501616597	37.0	37.0	37.0	35.0	37.0
7	36.61458299531971	37.0	37.0	37.0	35.0	37.0
8	36.49790425797062	37.0	37.0	37.0	35.0	37.0
9	38.27890111453018	39.0	39.0	39.0	37.0	39.0
10	38.090056496317004	39.0	39.0	39.0	35.0	39.0
11	37.5637887812385	39.0	35.0	39.0	35.0	39.0
12	37.47800730868889	39.0	35.0	39.0	35.0	39.0
13	37.47080839511935	39.0	35.0	39.0	35.0	39.0
14	38.80660488410419	41.0	36.0	41.0	35.0	41.0
15	38.87866098964867	41.0	36.0	41.0	35.0	41.0
16	38.86534710748937	41.0	36.0	41.0	35.0	41.0
17	38.91182280192183	41.0	36.0	41.0	35.0	41.0
18	38.89061269126969	41.0	36.0	41.0	35.0	41.0
19	38.83007194771105	41.0	36.0	41.0	35.0	41.0
20	38.74983309318544	41.0	35.0	41.0	35.0	41.0
21	38.68558484354946	41.0	35.0	41.0	35.0	41.0
22	38.648587799198154	41.0	35.0	41.0	35.0	41.0
23	38.60237998416198	41.0	35.0	41.0	35.0	41.0
24	38.530649752522244	40.0	35.0	41.0	35.0	41.0
25	38.485641181071976	40.0	35.0	41.0	35.0	41.0
26	38.413663782360416	40.0	35.0	41.0	35.0	41.0
27	38.38862879579236	40.0	36.0	41.0	35.0	41.0
28	38.412756582549314	40.0	36.0	41.0	35.0	41.0
29	38.414546127382174	40.0	36.0	41.0	35.0	41.0
30	38.36086599609366	40.0	35.0	41.0	35.0	41.0
31	38.30791286739348	40.0	35.0	41.0	35.0	41.0
32	38.24048803759701	40.0	35.0	41.0	35.0	41.0
33	38.14978255511377	40.0	35.0	41.0	35.0	41.0
34	38.06589418901929	40.0	35.0	41.0	35.0	41.0
35	37.99459822578229	40.0	35.0	41.0	35.0	41.0
36	37.90610274831833	40.0	35.0	41.0	35.0	41.0
37	37.822694217740384	40.0	35.0	41.0	35.0	41.0
38	37.77161900645741	40.0	35.0	41.0	35.0	41.0
39	37.645647339536445	40.0	35.0	41.0	34.0	41.0
40	37.526631561578085	40.0	35.0	41.0	34.0	41.0
41	37.48736582728821	40.0	35.0	41.0	34.0	41.0
42	37.41444463699235	39.0	35.0	41.0	34.0	41.0
43	37.42348211456263	39.0	35.0	41.0	34.0	41.0
44	37.393950482355514	39.0	35.0	41.0	34.0	41.0
45	37.36962454769461	39.0	35.0	41.0	34.0	41.0
46	37.29865722000562	39.0	35.0	41.0	34.0	41.0
47	37.21000087682174	39.0	35.0	41.0	34.0	41.0
48	37.17427971165683	39.0	35.0	41.0	34.0	41.0
49	37.099762291556345	39.0	35.0	41.0	34.0	41.0
50	37.01047008001171	38.0	35.0	41.0	34.0	41.0
51	36.92976036531017	38.0	35.0	41.0	34.0	41.0
52	36.64058731867223	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	8.0
14	9.0
15	16.0
16	26.0
17	68.0
18	117.0
19	241.0
20	385.0
21	651.0
22	1076.0
23	1598.0
24	2802.0
25	4372.0
26	6409.0
27	7605.0
28	8032.0
29	9028.0
30	10346.0
31	12655.0
32	16802.0
33	23947.0
34	73202.0
35	240964.0
36	63047.0
37	98046.0
38	163626.0
39	697182.0
40	6147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.45826570184057	21.99662665275719	23.760902449784695	19.78420519561755
2	27.478143319619473	24.1273725104649	26.1305994906149	22.263884679300723
3	27.634314246005804	24.96891425304799	25.096018884116617	22.30075261682959
4	26.658625682039585	26.44238901473544	22.543984347006	24.355000956218976
5	25.588005630990608	35.30029073199426	19.70957178650012	19.402131850515012
6	87.5148869832016	3.1854864600082986	6.65031313582521	2.649313420964877
7	88.16249232780982	2.987545679305557	5.056430727976068	3.7935312649085584
8	77.99274102068954	5.8716678185020434	9.858168906244282	6.277422254564133
9	49.044574993458355	23.376550748992173	13.911846966300356	13.667027291249113
10	38.80516123508971	23.247927214130222	23.281895426235472	14.66501612454459
11	38.40168515471761	18.61527064449159	24.21194783532045	18.771096365470346
12	23.00814753802955	30.40299969690965	26.391160532251508	20.19769223280929
13	16.69931159137622	32.4921828235455	28.48414091837066	22.32436466670763
14	14.967001815090034	26.67402184321737	38.00787482575757	20.351101515935028
15	17.397385966571687	18.933550030274514	42.3335747469817	21.3354892561721
16	18.15773539729345	21.66716261176888	27.877753099426748	32.297348891510914
17	20.232557978974228	24.4755466845437	29.430694145937657	25.86120119054441
18	24.403191631116265	27.943756373354837	25.365210060942562	22.287841934586336
19	29.060841072263226	22.55799968655349	24.318202059771625	24.062957181411658
20	23.215546946899813	28.561397888585642	25.88674639070486	22.33630877380968
21	21.952578442750788	24.573585020294626	24.975335073628862	28.49850146332572
22	20.758167732545896	28.851163307702983	23.68419780822183	26.706471151529293
23	21.04641424786991	21.91757461442282	34.495478844777004	22.540532292930262
24	21.73178506406667	22.286461112956044	30.36972189561955	25.612031927357737
25	19.94465666905779	30.407694490452652	25.91325816600652	23.734390674483038
26	19.220553806131264	28.854132074208117	26.83012372852218	25.095190391138438
27	21.800411899092317	25.7402412157306	31.741499144235796	20.71784774094129
28	18.170715120618222	25.919886109831936	29.515890840526836	26.39350792902301
29	20.511622030456785	26.515227355733483	26.949012470890555	26.024138142919178
30	25.057355878468364	24.96187206273349	22.852667022458373	27.12810503633977
31	25.76834093590709	26.461513394315016	24.406091356539882	23.364054313238007
32	24.63620528122849	27.546355908156027	22.476876415773678	25.340562394841804
33	18.83419991397481	25.472430860534946	25.46262702695985	30.230742198530393
34	22.429721357099115	23.784238335336674	27.332397596541867	26.45364271102234
35	24.057088689482903	24.62067103788767	23.56827783235859	27.753962440270836
36	21.768929165921598	29.66046286521869	24.839600307370898	23.731007661488814
37	22.40224300665625	26.273169323942824	26.191010436940292	25.13357723246063
38	20.981998918816664	27.14805790889753	24.4638097006862	27.40613347159961
39	26.01550800772984	23.84485640490661	26.22601426526826	23.913621322095285
40	17.48361827738359	22.390436981717233	31.423288799534387	28.702655941364792
41	21.02162849960612	26.111751275361378	23.632071791678204	29.234548433354302
42	22.294400837330237	24.33463383717213	26.307551782537164	27.06341354296047
43	22.1021214253117	27.395155939638766	26.517505711423468	23.985216923626066
44	20.075972806098815	28.3957683340318	24.61321460108408	26.915044258785304
45	21.531842092	25.877287762537343	24.48031051916822	28.110559626294435
46	23.207883386851677	24.60513679454686	26.092903060107858	26.094076758493607
47	21.931037625318194	21.913570231694965	30.017267174486832	26.138124968500005
48	19.673739465193975	22.68358541382879	27.927324595954328	29.715350525022906
49	19.509214567944362	25.492728938500274	29.484339066274607	25.513717427280753
50	18.995825085800806	26.023931019674635	27.347379511230567	27.63286438329399
51	20.90246359291169	23.116058748437084	27.664554239709254	28.316923418941975
52	22.56442050713436	21.142657515501448	29.65038286731754	26.642539110046652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	227.0
1	474.5
2	722.0
3	2907.0
4	5092.0
5	3444.5
6	1797.0
7	1895.5
8	1994.0
9	2104.0
10	2214.0
11	2100.5
12	1987.0
13	1931.5
14	1843.0
15	1810.0
16	1800.0
17	1790.0
18	1984.5
19	2179.0
20	1996.0
21	1813.0
22	2286.0
23	2759.0
24	3447.5
25	4136.0
26	5261.5
27	6387.0
28	9258.0
29	12129.0
30	13842.5
31	15556.0
32	16313.0
33	17070.0
34	20754.5
35	24439.0
36	26796.0
37	29153.0
38	32544.0
39	40239.0
40	44543.0
41	55963.0
42	67383.0
43	82243.0
44	97103.0
45	103947.5
46	110792.0
47	111555.5
48	112319.0
49	122848.5
50	133378.0
51	138715.0
52	144052.0
53	128509.0
54	112966.0
55	99817.0
56	86668.0
57	79455.0
58	72242.0
59	69442.5
60	66643.0
61	69298.0
62	71953.0
63	65387.5
64	47344.5
65	35867.0
66	28261.0
67	20655.0
68	17682.0
69	14709.0
70	11987.5
71	9266.0
72	8230.5
73	7195.0
74	5989.5
75	4784.0
76	3892.5
77	3001.0
78	2259.5
79	1518.0
80	1187.5
81	857.0
82	610.0
83	363.0
84	283.5
85	204.0
86	116.5
87	29.0
88	26.0
89	13.5
90	4.0
91	6.0
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1448413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.80471491003082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.25182868436067	15.3182109637081
2	12.021061687577816	5.242316460270565
3	4.5541520698408195	2.979059626194175
4	2.259086179671504	1.970349204197112
5	1.2722148178582662	1.387014070385814
6	0.8368072862513065	1.0947806586807776
7	0.55182435725382	0.8422660953231378
8	0.4244298155574513	0.7403656902037752
9	0.3255568344902071	0.6388806566763948
>10	5.350064519100998	31.734905876828385
>50	1.929103154256321	27.062668473186818
>100	0.21021607529511546	7.180555491305801
>500	0.007621126596659291	1.119821840407262
>1k	0.005715844947494469	2.323008888002451
>5k	3.175469415274705E-4	0.3657960046294172
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	5283	0.3647440336423382	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	4419	0.30509253921360824	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	4175	0.28824651532401324	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3225	0.22265748788501621	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2700	0.18641092008978102	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1816	0.1253786040307564	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1640	0.11322737368416329	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1535	0.10597806012511625	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1525	0.1052876493099689	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	1523	0.10514956714693943	No Hit
GACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1507	0.1040449098427037	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31365363332143525	0.0
2	0.0	0.0	0.0	1.1671394830065733	0.0
3	6.904108151473372E-5	0.0	0.0	1.839530575878565	0.0
4	6.904108151473372E-5	0.0	0.0	2.2577814476948217	0.0
5	6.904108151473372E-5	0.0	0.0	4.479937697328041	0.0
6	6.904108151473372E-5	0.0	0.0	4.837294335248303	0.0
7	6.904108151473372E-5	0.0	0.0	6.072853530035977	0.0
8	6.904108151473372E-5	0.0	0.0	7.610950744021215	0.0
9	6.904108151473372E-5	0.0	0.0	8.674321481511143	0.0
10	6.904108151473372E-5	0.0	0.0	9.50854486945367	0.0
11	6.904108151473372E-5	0.0	0.0	10.310802236654876	0.0
12	1.3808216302946743E-4	0.0	0.0	11.007012502649452	0.0
13	1.3808216302946743E-4	0.0	0.0	11.32128750570452	0.0
14	1.3808216302946743E-4	0.0	0.0	11.543461706018933	0.0
15	1.3808216302946743E-4	0.0	0.0	12.29207415288319	0.0
16	1.3808216302946743E-4	0.0	0.0	13.09702412226347	0.0
17	1.3808216302946743E-4	0.0	0.0	14.082309396560236	0.0
18	1.3808216302946743E-4	0.0	0.0	14.560971214701883	0.0
19	1.3808216302946743E-4	0.0	0.0	15.122689453905757	0.0
20	1.3808216302946743E-4	0.0	0.0	15.827115608600586	0.0
21	1.3808216302946743E-4	0.0	0.0	16.47637793916514	0.0
22	2.0712324454420113E-4	0.0	0.0	17.20020463776561	0.0
23	2.0712324454420113E-4	0.0	0.0	17.729404527576044	0.0
24	2.0712324454420113E-4	0.0	0.0	18.20744497598406	0.0
25	2.7616432605893486E-4	0.0	0.0	18.63315228460391	0.0
26	2.7616432605893486E-4	0.0	0.0	19.027791106542125	0.0
27	3.4520540757366857E-4	0.0	0.0	19.449701155678664	0.0
28	3.4520540757366857E-4	0.0	0.0	19.862152576647684	0.0
29	3.4520540757366857E-4	0.0	0.0	20.378648907459407	0.0
30	3.4520540757366857E-4	0.0	0.0	20.881060857642122	0.0
31	3.4520540757366857E-4	0.0	0.0	21.37104541315219	0.0
32	4.1424648908840227E-4	0.0	0.0	21.790608065517226	0.0
33	4.1424648908840227E-4	0.0	0.0	22.1986408572693	0.0
34	4.1424648908840227E-4	0.0	0.0	22.75649279590835	0.0
35	4.8328757060313597E-4	0.0	0.0	23.21844667232343	0.0
36	4.8328757060313597E-4	0.0	0.0	23.721549033321295	0.0
37	4.8328757060313597E-4	0.0	0.0	24.14325195921329	0.0
38	4.8328757060313597E-4	0.0	0.0	24.549765847172043	0.0
39	4.8328757060313597E-4	0.0	0.0	25.014757531173775	0.0
40	4.8328757060313597E-4	0.0	0.0	25.494869212027233	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	6.312002E-4	46.0	25
TACCCGC	20	6.312002E-4	46.0	21
CAAACGT	20	6.312002E-4	46.0	12
TATGCGC	20	6.312002E-4	46.0	33
TGCGCAC	25	3.4175995E-5	46.0	20
GAACGAT	30	1.8617411E-6	46.0	8
CGTAAGT	20	6.312002E-4	46.0	24
CGGTATG	20	6.312002E-4	46.0	5
TCTACGG	80	0.0	43.125	1
ACTACGG	130	0.0	42.46154	1
CGGGCAC	170	0.0	41.941177	5
CACTAGG	200	0.0	41.4	1
CCAACGG	50	8.731149E-10	41.4	1
TTAATCG	240	0.0	41.208332	19
ATAAGCG	95	0.0	41.157894	32
CGAATAT	310	0.0	40.80645	14
GCGTAGG	260	0.0	40.69231	1
CGTAGGG	560	0.0	40.660717	2
ATGGGCG	210	0.0	40.523808	4
CGTTTTT	1915	0.0	40.35509	1
>>END_MODULE