FastQCFastQC Report
Thu 26 May 2016
SRR1544855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544855_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1045155
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT71890.6878405595342317No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT57500.5501576321215513Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA45210.43256741822983197No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT41850.4004190765962944No Hit
TGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTTG28940.27689672823648165Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21850.20905990020618953No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC16770.16045466940310288No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT15280.14619841076204007No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC14890.14246690682243304No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC13340.1276365706521999No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC11880.11366735077572226No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.1109883223062608No Hit
CTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTTGAAA10490.10036788801660998Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGTC253.416941E-546.024
CACGTCG253.416941E-546.027
CCACGTC206.31119E-446.017
TGCGTTA206.31119E-446.021
ATAGCGG1200.044.0833321
ACGTAGG1050.043.8095251
ACTACGG900.043.4444471
GTCGGCG6800.043.294123
TCGGCGT6900.042.3333324
CGTTGAT2200.041.8181824
ATCACGG554.7293724E-1141.818181
ACGGGTA554.7293724E-1141.818184
TATACGG554.7293724E-1141.818181
CACGACG508.712959E-1041.425
GTCCCCC7150.041.1748289
GCGTCCC7100.041.1408467
TAGGGTA1850.041.0270274
TTAATCG2250.040.8888919
TAATCGT2250.040.8888920
CGTTTTT11850.040.5654031
Produced by FastQC (version 0.11.5)