##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544855_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1045155
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82073568035363	34.0	33.0	34.0	31.0	34.0
2	33.19390042625257	34.0	33.0	34.0	31.0	34.0
3	33.302662284541526	34.0	34.0	34.0	31.0	34.0
4	36.55393314867173	37.0	37.0	37.0	35.0	37.0
5	36.55799666078237	37.0	37.0	37.0	35.0	37.0
6	36.483960752232925	37.0	37.0	37.0	35.0	37.0
7	36.358433916500424	37.0	37.0	37.0	35.0	37.0
8	36.38514383034095	37.0	37.0	37.0	35.0	37.0
9	38.21613923293674	39.0	39.0	39.0	37.0	39.0
10	38.10194086044654	39.0	39.0	39.0	35.0	39.0
11	37.65236926580268	39.0	37.0	39.0	35.0	39.0
12	37.61184417622267	39.0	37.0	39.0	35.0	39.0
13	37.60848199549349	39.0	37.0	39.0	35.0	39.0
14	38.92366299735446	41.0	38.0	41.0	35.0	41.0
15	38.99105491529964	41.0	38.0	41.0	35.0	41.0
16	38.99261544938311	41.0	38.0	41.0	35.0	41.0
17	39.04509953069162	41.0	38.0	41.0	35.0	41.0
18	39.03664719587047	41.0	38.0	41.0	35.0	41.0
19	38.988327090240205	41.0	37.0	41.0	35.0	41.0
20	38.920399366601124	41.0	37.0	41.0	35.0	41.0
21	38.86661787007669	41.0	36.0	41.0	35.0	41.0
22	38.830739938095306	41.0	36.0	41.0	35.0	41.0
23	38.800165525687575	41.0	36.0	41.0	35.0	41.0
24	38.71526711349034	41.0	36.0	41.0	35.0	41.0
25	38.65528175246734	41.0	36.0	41.0	35.0	41.0
26	38.579055738144106	40.0	35.0	41.0	35.0	41.0
27	38.571084671651576	40.0	36.0	41.0	35.0	41.0
28	38.614245733886364	40.0	36.0	41.0	35.0	41.0
29	38.65386378097028	40.0	36.0	41.0	35.0	41.0
30	38.63191775382599	41.0	36.0	41.0	35.0	41.0
31	38.604446230463424	41.0	35.0	41.0	35.0	41.0
32	38.56720964832967	40.0	36.0	41.0	35.0	41.0
33	38.50348799938765	40.0	36.0	41.0	35.0	41.0
34	38.445452588372056	40.0	35.0	41.0	35.0	41.0
35	38.37023120972487	40.0	35.0	41.0	35.0	41.0
36	38.319154575158706	40.0	35.0	41.0	35.0	41.0
37	38.25610746731346	40.0	35.0	41.0	35.0	41.0
38	38.174419105300174	40.0	35.0	41.0	35.0	41.0
39	38.122954968401814	40.0	35.0	41.0	35.0	41.0
40	38.04551860728792	40.0	35.0	41.0	35.0	41.0
41	38.00792418349432	40.0	35.0	41.0	35.0	41.0
42	37.95150862790687	40.0	35.0	41.0	35.0	41.0
43	37.87688811707354	40.0	35.0	41.0	35.0	41.0
44	37.74369925991839	40.0	35.0	41.0	35.0	41.0
45	37.725957393879376	40.0	35.0	41.0	35.0	41.0
46	37.65558218637427	40.0	35.0	41.0	35.0	41.0
47	37.575613186560844	39.0	35.0	41.0	35.0	41.0
48	37.50692863737915	39.0	35.0	41.0	35.0	41.0
49	37.45545110533844	39.0	35.0	41.0	35.0	41.0
50	37.36308490128258	39.0	35.0	41.0	34.0	41.0
51	37.28076888117074	39.0	35.0	41.0	34.0	41.0
52	36.833859092670465	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	5.0
14	8.0
15	15.0
16	28.0
17	44.0
18	77.0
19	143.0
20	290.0
21	441.0
22	624.0
23	1013.0
24	1570.0
25	2500.0
26	3679.0
27	4289.0
28	4458.0
29	5037.0
30	5908.0
31	7612.0
32	10156.0
33	15086.0
34	46796.0
35	163458.0
36	42856.0
37	68573.0
38	116764.0
39	537758.0
40	5964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.45522912869383	21.734288215623522	22.771263592481496	22.039219063201152
2	27.24591089359951	24.21621673340318	25.902856514105565	22.635015858891745
3	27.8552941908138	24.57281455860614	25.068626184632905	22.503265065947158
4	26.41943061077065	25.869942735766465	23.21971382235171	24.490912831111174
5	25.34188708851797	34.35739196578498	19.97349675406997	20.32722419162708
6	85.15454645483206	3.183833976778564	7.975754792351373	3.6858647760380037
7	86.07488841368027	2.9950581492697257	6.078811276796265	4.851242160253742
8	74.44130296463204	5.959785869081619	11.699125966961839	7.899785199324502
9	47.29040190211021	22.30750462850008	15.094603192827858	15.30749027656185
10	36.836737134683375	22.601719362199866	25.464452640995834	15.097090862120929
11	35.869990575560564	18.44750300194708	25.210901732278945	20.471604690213415
12	22.230673919179452	28.640249532366013	28.045026814204594	21.084049734249945
13	16.362931813941472	31.110887858738657	29.57427367232611	22.951906654993756
14	14.63007879214088	26.34087766886251	37.099473283866985	21.929570255129622
15	16.65207552946692	18.829742956786312	42.0189349905038	22.499246523242963
16	17.42239189402529	22.66946051064196	28.04024283479484	31.86790476053791
17	19.105108811611675	24.69949433337639	29.77587056465309	26.419526290358846
18	23.167472767197207	27.96102013576934	26.297152097057374	22.57435499997608
19	27.223808908726454	22.386535968349193	24.93754514880568	25.45210997411867
20	21.93598078753869	29.05607302266171	26.566968535767423	22.440977654032178
21	21.218479555663993	24.327109376121246	25.29653496371352	29.157876104501245
22	19.87217207017141	28.830077835344998	23.88880118259971	27.408948911883886
23	19.926613755854394	22.005731207332886	34.836555343465804	23.23109969334692
24	21.883835411972388	22.394955772110357	29.616468370720135	26.104740445197123
25	19.557386225009687	29.644215451296695	25.788136687859698	25.01026163583392
26	18.8918390095249	27.60958900832891	26.512239811319855	26.986332170826337
27	22.348359812659364	24.76407805540805	30.812080504805508	22.07548162712708
28	17.973027924087816	25.505212145566926	28.850553267218736	27.671206663126526
29	20.54183350794858	25.628256095985762	26.09708607814152	27.732824317924138
30	24.196506738234998	24.64438289057604	22.060364252192258	29.098746118996704
31	25.34791490257426	25.155311891537618	24.159861455956293	25.33691174993183
32	23.551339275035758	27.182666685802587	21.901440456200277	27.364553582961378
33	17.990058890786536	24.413986442202354	25.35308160033679	32.24287306667432
34	22.864933909324453	22.708593462213738	27.281120982055295	27.145351646406514
35	23.016873095378198	23.955011457630686	23.516320545756372	29.511794901234744
36	21.829202367113012	29.411139974453548	23.80642105716377	24.95323660126967
37	22.528237438466064	25.499088651922442	26.433878228588103	25.53879568102339
38	20.70802895264339	26.51998985796365	23.639268816587013	29.132712372805948
39	26.351976501093137	23.029885519372726	25.782587271744383	24.835550707789753
40	17.427654271376017	21.54206792293966	31.074912333577316	29.955365472107008
41	20.631868000440125	25.673608220790218	23.521295884342514	30.17322789442714
42	21.527237586769427	23.17866727901603	26.94337203572676	28.350723098487784
43	22.710220015213054	26.086752682616453	26.40785338059905	24.795173921571443
44	20.199109223033904	26.5131966072018	25.26142055484593	28.026273614918363
45	21.095626964421545	24.094703656395463	24.919270347460422	29.89039903172257
46	23.506943946113257	22.811544699111614	26.328535001985355	27.352976352789778
47	20.798159124723128	20.985978156350015	31.092613057393404	27.123249661533457
48	19.384875927494008	21.197334366672884	27.80659328042252	31.611196425410586
49	19.722337835057957	23.601188340485383	30.00397070291009	26.672503121546566
50	19.18739325745942	23.922289038467976	28.26470714870043	28.625610555372173
51	20.718362348168455	21.4659069707364	27.871368361630573	29.94436231946458
52	21.886610120030042	20.16648248345939	31.40070133138147	26.5462060651291
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	114.0
1	243.0
2	372.0
3	1403.0
4	2434.0
5	1762.0
6	1090.0
7	1114.0
8	1138.0
9	1191.0
10	1244.0
11	1251.0
12	1258.0
13	1188.5
14	1111.0
15	1103.0
16	1106.0
17	1109.0
18	1232.5
19	1356.0
20	1281.0
21	1206.0
22	1524.5
23	1843.0
24	2186.0
25	2529.0
26	3279.5
27	4030.0
28	5614.0
29	7198.0
30	8253.5
31	9309.0
32	9842.0
33	10375.0
34	12948.5
35	15522.0
36	17204.5
37	18887.0
38	20984.0
39	25708.5
40	28336.0
41	36166.5
42	43997.0
43	53303.5
44	62610.0
45	68196.0
46	73782.0
47	77381.5
48	80981.0
49	96026.0
50	111071.0
51	116348.5
52	121626.0
53	106816.5
54	92007.0
55	79782.5
56	67558.0
57	60007.5
58	52457.0
59	49245.0
60	46033.0
61	47166.0
62	48299.0
63	45591.5
64	33544.0
65	24204.0
66	19231.0
67	14258.0
68	12021.0
69	9784.0
70	8171.0
71	6558.0
72	5726.0
73	4894.0
74	4180.0
75	3466.0
76	2744.5
77	2023.0
78	1525.0
79	1027.0
80	815.5
81	604.0
82	412.0
83	220.0
84	174.5
85	129.0
86	71.5
87	14.0
88	13.0
89	7.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1045155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.57553877551934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.61981394221797	18.061397897991572
2	11.663032367927824	5.965766731321506
3	4.326768327086572	3.3197829336627476
4	2.045548668927752	2.092640371974948
5	1.2655459824044906	1.6183510172594384
6	0.7811376903341143	1.1986810372895857
7	0.5720312269938452	1.0241004778752298
8	0.43907243386286565	0.8983611246017098
9	0.3779256461465435	0.8699086815555713
>10	7.365608641701122	47.658397104373556
>50	0.41684524590612454	7.02753944166963
>100	0.11238658196072997	4.99598105643016
>500	0.00864512168928681	1.5984832212106992
>1k	0.004886373128727328	2.42675670172257
>5k	7.517497121118965E-4	1.243852201061176
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	7189	0.6878405595342317	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	5750	0.5501576321215513	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4521	0.43256741822983197	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	4185	0.4004190765962944	No Hit
TGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTTG	2894	0.27689672823648165	Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2185	0.20905990020618953	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1677	0.16045466940310288	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	1528	0.14619841076204007	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1489	0.14246690682243304	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1334	0.1276365706521999	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1188	0.11366735077572226	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1160	0.1109883223062608	No Hit
CTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTTGAAA	1049	0.10036788801660998	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5621175806459329	0.0
2	0.0	0.0	0.0	1.9228726839559682	0.0
3	0.0	0.0	0.0	2.8979433672517474	0.0
4	0.0	0.0	0.0	3.536221900100942	0.0
5	0.0	0.0	0.0	6.6681018604895925	0.0
6	0.0	0.0	0.0	7.160851739694112	0.0
7	0.0	0.0	0.0	8.861269381096584	0.0
8	0.0	0.0	0.0	10.933784940989614	0.0
9	0.0	0.0	0.0	12.429543943242868	0.0
10	1.9135917639010483E-4	0.0	0.0	13.622859767211562	0.0
11	1.9135917639010483E-4	0.0	0.0	14.71590338275184	0.0
12	1.9135917639010483E-4	0.0	0.0	15.670881352526658	0.0
13	1.9135917639010483E-4	0.0	0.0	16.091967220173085	0.0
14	1.9135917639010483E-4	0.0	0.0	16.404074036865346	0.0
15	1.9135917639010483E-4	0.0	0.0	17.40784859661964	0.0
16	1.9135917639010483E-4	0.0	0.0	18.414302184843397	0.0
17	1.9135917639010483E-4	0.0	0.0	19.592022235936298	0.0
18	1.9135917639010483E-4	0.0	0.0	20.188106070391473	0.0
19	1.9135917639010483E-4	0.0	0.0	20.874033038161805	0.0
20	1.9135917639010483E-4	0.0	0.0	21.726251130215136	0.0
21	1.9135917639010483E-4	0.0	0.0	22.513120063531247	0.0
22	1.9135917639010483E-4	0.0	0.0	23.369452377876964	0.0
23	1.9135917639010483E-4	0.0	0.0	23.98821227473437	0.0
24	1.9135917639010483E-4	0.0	0.0	24.531098258153097	0.0
25	1.9135917639010483E-4	0.0	0.0	25.022987021063862	0.0
26	1.9135917639010483E-4	0.0	0.0	25.488372538044597	0.0
27	1.9135917639010483E-4	0.0	0.0	25.96715319737264	0.0
28	1.9135917639010483E-4	0.0	0.0	26.429764006295716	0.0
29	1.9135917639010483E-4	0.0	0.0	26.991020470647893	0.0
30	1.9135917639010483E-4	0.0	0.0	27.531131746008967	0.0
31	1.9135917639010483E-4	0.0	0.0	28.05861331572829	0.0
32	1.9135917639010483E-4	0.0	0.0	28.51825805741732	0.0
33	1.9135917639010483E-4	0.0	0.0	28.966612607699336	0.0
34	1.9135917639010483E-4	0.0	0.0	29.55810382192115	0.0
35	1.9135917639010483E-4	0.0	0.0	30.045591323774943	0.0
36	1.9135917639010483E-4	0.0	0.0	30.57374265061163	0.0
37	1.9135917639010483E-4	0.0	0.0	31.028316374126327	0.0
38	1.9135917639010483E-4	0.0	0.0	31.468442479823565	0.0
39	1.9135917639010483E-4	0.0	0.0	31.9455009065641	0.0
40	1.9135917639010483E-4	0.0	0.0	32.46542378881601	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGTC	25	3.416941E-5	46.0	24
CACGTCG	25	3.416941E-5	46.0	27
CCACGTC	20	6.31119E-4	46.0	17
TGCGTTA	20	6.31119E-4	46.0	21
ATAGCGG	120	0.0	44.083332	1
ACGTAGG	105	0.0	43.809525	1
ACTACGG	90	0.0	43.444447	1
GTCGGCG	680	0.0	43.29412	3
TCGGCGT	690	0.0	42.333332	4
CGTTGAT	220	0.0	41.81818	24
ATCACGG	55	4.7293724E-11	41.81818	1
ACGGGTA	55	4.7293724E-11	41.81818	4
TATACGG	55	4.7293724E-11	41.81818	1
CACGACG	50	8.712959E-10	41.4	25
GTCCCCC	715	0.0	41.174828	9
GCGTCCC	710	0.0	41.140846	7
TAGGGTA	185	0.0	41.027027	4
TTAATCG	225	0.0	40.88889	19
TAATCGT	225	0.0	40.88889	20
CGTTTTT	1185	0.0	40.565403	1
>>END_MODULE