FastQCFastQC Report
Thu 26 May 2016
SRR1544854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544854_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences928881
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT49520.5331145754946005TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT41930.4514033552198828Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT29950.322430968014202TruSeq Adapter, Index 13 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC15070.16223821996574372No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT12970.13963037245890486TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.13952271604220562No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC11940.1285417615388839TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC11700.1259580075381023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG351.0192889E-746.00000423
GCGATAG351.0192889E-746.0000048
ACCGGTA253.4166453E-546.041
ACGCCTA206.310826E-446.036
GCGACGG501.6370905E-1146.01
TCCGCGA301.8610153E-646.012
CTCGTCT206.310826E-446.09
ACACCGT206.310826E-446.046
TATGGGT1100.046.03
TGGCGTA206.310826E-446.010
CCTACGT206.310826E-446.014
CCGCTTC405.6097633E-946.040
TACGGGT501.6370905E-1146.03
TACGGGA1600.046.03
CGACACT206.310826E-446.013
CGTTCCC206.310826E-446.08
CGCCTAC253.4166453E-546.039
GCCGATT253.4166453E-546.08
CTAACGG750.046.01
CCGTAGG501.6370905E-1146.01
Produced by FastQC (version 0.11.5)