##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544854_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	928881
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88943363035739	34.0	31.0	34.0	31.0	34.0
2	33.20372362014079	34.0	33.0	34.0	31.0	34.0
3	33.25241554084969	34.0	34.0	34.0	31.0	34.0
4	36.55363927133831	37.0	37.0	37.0	35.0	37.0
5	36.534464586960006	37.0	37.0	37.0	35.0	37.0
6	36.67665287587969	37.0	37.0	37.0	35.0	37.0
7	36.663862217011655	37.0	37.0	37.0	35.0	37.0
8	36.57515117652315	37.0	37.0	37.0	35.0	37.0
9	38.32459916824652	39.0	39.0	39.0	37.0	39.0
10	38.12132232223503	39.0	39.0	39.0	35.0	39.0
11	37.35359104126363	39.0	35.0	39.0	35.0	39.0
12	37.229611758664454	39.0	35.0	39.0	35.0	39.0
13	37.183161244551236	39.0	35.0	39.0	35.0	39.0
14	38.44862581966904	40.0	35.0	41.0	35.0	41.0
15	38.518501293491845	41.0	35.0	41.0	35.0	41.0
16	38.50024922460466	40.0	35.0	41.0	35.0	41.0
17	38.58063734751814	40.0	36.0	41.0	35.0	41.0
18	38.570858915189355	40.0	36.0	41.0	35.0	41.0
19	38.486305565513774	40.0	35.0	41.0	35.0	41.0
20	38.40646541376129	40.0	35.0	41.0	35.0	41.0
21	38.34992749340336	40.0	35.0	41.0	35.0	41.0
22	38.330725894920874	40.0	35.0	41.0	35.0	41.0
23	38.296825965866454	40.0	35.0	41.0	35.0	41.0
24	38.248978071464485	40.0	35.0	41.0	35.0	41.0
25	38.2141210768656	40.0	35.0	41.0	35.0	41.0
26	38.160662130025266	40.0	35.0	41.0	35.0	41.0
27	38.13319036561196	40.0	35.0	41.0	35.0	41.0
28	38.1745358124453	40.0	35.0	41.0	35.0	41.0
29	38.16622689020445	40.0	35.0	41.0	35.0	41.0
30	38.05673277847216	40.0	35.0	41.0	35.0	41.0
31	38.00733140197722	40.0	35.0	41.0	35.0	41.0
32	37.93578187087474	40.0	35.0	41.0	35.0	41.0
33	37.81105222305118	40.0	35.0	41.0	35.0	41.0
34	37.7637329216552	40.0	35.0	41.0	35.0	41.0
35	37.73073945962938	40.0	35.0	41.0	35.0	41.0
36	37.674005604593056	40.0	35.0	41.0	34.0	41.0
37	37.60771831913883	40.0	35.0	41.0	34.0	41.0
38	37.61203318832014	40.0	35.0	41.0	35.0	41.0
39	37.50414315719667	40.0	35.0	41.0	34.0	41.0
40	37.43679222634546	39.0	35.0	41.0	34.0	41.0
41	37.41331236186336	39.0	35.0	41.0	34.0	41.0
42	37.31563677155631	39.0	35.0	41.0	34.0	41.0
43	37.31605663158144	39.0	35.0	41.0	34.0	41.0
44	37.27838334512171	39.0	35.0	41.0	34.0	41.0
45	37.24978657115389	39.0	35.0	41.0	34.0	41.0
46	37.21400803762807	39.0	35.0	41.0	34.0	41.0
47	37.11568220256416	39.0	35.0	41.0	34.0	41.0
48	37.13193186210074	39.0	35.0	41.0	34.0	41.0
49	37.07078301741558	39.0	35.0	41.0	34.0	41.0
50	36.97181985636481	38.0	35.0	41.0	34.0	41.0
51	36.88556553530538	38.0	35.0	41.0	34.0	41.0
52	36.607790448938026	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	7.0
16	15.0
17	21.0
18	49.0
19	115.0
20	245.0
21	405.0
22	623.0
23	1088.0
24	1938.0
25	3094.0
26	4361.0
27	4939.0
28	5412.0
29	6088.0
30	7304.0
31	8781.0
32	11515.0
33	16240.0
34	48711.0
35	202407.0
36	31030.0
37	44929.0
38	84710.0
39	441486.0
40	3364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.825800075574804	22.95105616327603	26.16664567366541	20.056498087483757
2	24.508629200080527	24.824385470259376	27.53086778607809	23.136117543582007
3	24.306988731602864	25.853580813904042	27.511812600322322	22.32761785417077
4	26.292496024786814	26.67112364231802	24.469334607985306	22.567045724909864
5	24.450925360729737	33.99531263961691	20.94121851991805	20.612543479735297
6	88.15844010158459	2.0415962862842494	7.181974870839214	2.6179887412919416
7	89.82571502700561	1.598267162316809	4.7309612318477825	3.845056578829796
8	80.99304431891706	3.1305409411969887	9.93119678408752	5.945217955798428
9	48.12327951589063	17.612482115577777	14.258554109729879	20.005684258801722
10	41.45784013237433	17.44550701327727	25.877480538411273	15.219172315937133
11	39.2877020845512	18.567179218866574	23.250125688866497	18.894993007715737
12	20.134118363923903	35.269211018418936	27.087646318527348	17.509024299129813
13	14.470206624960571	38.3049066565039	29.812645538018323	17.412241180517203
14	14.058097861835908	24.396774183130024	44.861936028404074	16.68319192663
15	16.22931247382603	18.757623420007512	46.07974541410579	18.933318692060663
16	15.517380590194009	20.494767359866334	28.239462320792434	35.74838972914722
17	19.453622153968052	22.343228034592162	33.55079929506578	24.652350516374003
18	23.476527133184984	27.19637929939357	27.921552922279602	21.405540645141844
19	27.166343159134488	23.369516654985944	25.344904245000166	24.119235940879403
20	20.14197728234295	30.39420550102758	27.740044203724697	21.723773012904772
21	19.212041154894976	24.159930066391713	28.17992832235776	28.44810045635555
22	17.63885793766909	28.837601371973378	24.96272396571789	28.560816724639647
23	18.527884626771353	21.95566493447492	37.989903981241945	21.52654645751178
24	19.126346647202386	21.971813396979805	34.604863270967975	24.296976684849835
25	16.55518844717461	31.041435878223368	29.35639764404698	23.046978030555042
26	17.226533861711026	28.787110512541435	28.87495814856801	25.11139747717953
27	17.55025670672562	26.83691452403483	36.845300958895706	18.767527810343843
28	16.52030776816406	23.51743657153069	34.6996009176633	25.262654742641956
29	21.45129462223902	25.939921260096828	29.965625306147935	22.643158811516223
30	26.712248393497124	23.697761069501905	27.340208272103744	22.249782264897224
31	27.981840515631173	24.64589113137205	26.790945234104264	20.581323118892517
32	27.85448297467598	24.789935416915622	25.23003484838209	22.12554676002631
33	17.93308292450809	23.177134638344416	32.94910758213378	25.940674855013725
34	17.84523528848152	22.27992606157301	32.632705373454726	27.242133276490744
35	26.8422973448698	21.100980642299714	29.261982966601753	22.79473904622874
36	22.464341503378797	24.340146907946227	28.989396919519294	24.20611466915568
37	19.11934898011694	26.650130641061665	28.53293371271455	25.697586666106854
38	18.92502914797482	24.73524595723241	27.115636986869145	29.224087907923618
39	25.10870606676205	20.873610290230936	25.974909595524075	28.042774047482936
40	16.61364588144229	19.734282432302955	30.8548673080836	32.79720437817115
41	21.257728385013795	23.221058456357703	25.363528805089135	30.157684353539366
42	22.19293967688003	20.50940863253743	28.950532953090867	28.347118737491673
43	22.276588712655336	24.01577812443144	29.527894315848858	24.17973884706437
44	19.136143381122018	28.312561027731213	26.423513883909777	26.127781707236984
45	20.98471171226454	25.088036034755795	28.4971917823704	25.430060470609263
46	22.72766909862512	20.78866937745524	28.481258632698914	28.00240289122073
47	20.829794128634347	18.426364625823975	34.02760956462669	26.716231680914994
48	20.444276500434395	17.4268824531883	31.18257344051606	30.946267605861244
49	20.22874835420253	23.009082971876914	31.030131954469947	25.73203671945061
50	18.884012053212412	24.95561864221574	29.189207228913066	26.97116207565878
51	19.533395558742185	19.519723193821385	30.476670316219195	30.47021093121724
52	21.612779247287868	18.53929620694147	33.97679573594465	25.871128809826015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	92.0
1	209.5
2	327.0
3	1615.0
4	2903.0
5	1987.5
6	1072.0
7	1154.0
8	1236.0
9	1296.5
10	1357.0
11	1390.5
12	1424.0
13	1343.5
14	1263.0
15	1263.0
16	1231.5
17	1200.0
18	1118.5
19	1037.0
20	1019.5
21	1002.0
22	1770.0
23	2538.0
24	2683.5
25	2829.0
26	3369.5
27	3910.0
28	4225.0
29	4540.0
30	6183.0
31	7826.0
32	8745.5
33	9665.0
34	12238.5
35	14812.0
36	16141.5
37	17471.0
38	19320.5
39	24129.5
40	27089.0
41	36869.5
42	46650.0
43	60958.5
44	75267.0
45	80717.5
46	86168.0
47	87916.0
48	89664.0
49	97171.0
50	104678.0
51	107227.5
52	109777.0
53	97529.0
54	85281.0
55	71833.5
56	58386.0
57	49352.0
58	40318.0
59	35232.5
60	30147.0
61	28286.5
62	26426.0
63	22735.5
64	16152.0
65	13259.0
66	10339.5
67	7420.0
68	6264.5
69	5109.0
70	4004.0
71	2899.0
72	1977.0
73	1055.0
74	861.5
75	668.0
76	518.5
77	369.0
78	279.5
79	190.0
80	107.5
81	25.0
82	21.0
83	17.0
84	23.0
85	29.0
86	17.5
87	6.0
88	3.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	928881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.504588144854246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.57455347009564	15.883120483870291
2	13.272687419613979	6.239381027468297
3	5.502878984805013	3.880287124464464
4	2.8092762719017625	2.6412352702465
5	1.6271443482020316	1.9122678878358033
6	0.9991284708374933	1.4090461926499975
7	0.6512047999876374	1.0714410435163122
8	0.4754760126069211	0.8940694279266566
9	0.34365635314118137	0.7269750939551451
>10	4.984375729529325	32.54104649282748
>50	1.5651243721106232	23.18783474524188
>100	0.18623695629803644	6.878915319228277
>500	0.004587117150197942	0.7285282489620141
>1k	0.0036696937201583534	2.0058516418069128
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	4952	0.5331145754946005	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT	4193	0.4514033552198828	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	2995	0.322430968014202	TruSeq Adapter, Index 13 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1507	0.16223821996574372	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	1297	0.13963037245890486	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1296	0.13952271604220562	No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1194	0.1285417615388839	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1170	0.1259580075381023	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46421446880709155	0.0
2	0.0	0.0	0.0	1.5882551155637805	0.0
3	0.0	0.0	0.0	2.5049495037577474	0.0
4	0.0	0.0	0.0	3.0496909722558647	0.0
5	0.0	0.0	0.0	5.85801625827205	0.0
6	0.0	0.0	0.0	6.319216347411563	0.0
7	0.0	0.0	0.0	8.112449280370683	0.0
8	0.0	0.0	0.0	10.219285355174668	0.0
9	0.0	0.0	0.0	11.959981956784562	0.0
10	0.0	0.0	0.0	13.163257726231885	0.0
11	0.0	0.0	0.0	14.28492993182119	0.0
12	0.0	0.0	0.0	15.279244596455305	0.0
13	0.0	0.0	0.0	15.644092192649005	0.0
14	0.0	0.0	0.0	15.929274040485272	0.0
15	0.0	0.0	0.0	16.956423912212653	0.0
16	0.0	0.0	0.0	18.133539172402063	0.0
17	0.0	0.0	0.0	19.721148349465647	0.0
18	0.0	0.0	0.0	20.418438960426577	0.0
19	0.0	0.0	0.0	21.19302687857756	0.0
20	0.0	0.0	0.0	22.167425106122312	0.0
21	0.0	0.0	0.0	22.964082589696634	0.0
22	0.0	0.0	0.0	23.92577736007088	0.0
23	0.0	0.0	0.0	24.60487403660964	0.0
24	0.0	0.0	0.0	25.166732875362936	0.0
25	0.0	0.0	0.0	25.70781402569328	0.0
26	0.0	0.0	0.0	26.166107391581914	0.0
27	0.0	0.0	0.0	26.676614119569678	0.0
28	0.0	0.0	0.0	27.18238396522267	0.0
29	0.0	0.0	0.0	27.898729761939364	0.0
30	0.0	0.0	0.0	28.527120266212787	0.0
31	0.0	0.0	0.0	29.023631660029647	0.0
32	0.0	0.0	0.0	29.5215425872636	0.0
33	0.0	0.0	0.0	30.02946556125058	0.0
34	0.0	0.0	0.0	30.761852164055462	0.0
35	0.0	0.0	0.0	31.284308754296838	0.0
36	1.0765641669923274E-4	0.0	0.0	31.785449374031764	0.0
37	1.0765641669923274E-4	0.0	0.0	32.33503538128135	0.0
38	1.0765641669923274E-4	0.0	0.0	32.82487207726286	0.0
39	1.0765641669923274E-4	0.0	0.0	33.42376472336069	0.0
40	1.0765641669923274E-4	0.0	0.0	33.99854233211789	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAG	35	1.0192889E-7	46.000004	23
GCGATAG	35	1.0192889E-7	46.000004	8
ACCGGTA	25	3.4166453E-5	46.0	41
ACGCCTA	20	6.310826E-4	46.0	36
GCGACGG	50	1.6370905E-11	46.0	1
TCCGCGA	30	1.8610153E-6	46.0	12
CTCGTCT	20	6.310826E-4	46.0	9
ACACCGT	20	6.310826E-4	46.0	46
TATGGGT	110	0.0	46.0	3
TGGCGTA	20	6.310826E-4	46.0	10
CCTACGT	20	6.310826E-4	46.0	14
CCGCTTC	40	5.6097633E-9	46.0	40
TACGGGT	50	1.6370905E-11	46.0	3
TACGGGA	160	0.0	46.0	3
CGACACT	20	6.310826E-4	46.0	13
CGTTCCC	20	6.310826E-4	46.0	8
CGCCTAC	25	3.4166453E-5	46.0	39
GCCGATT	25	3.4166453E-5	46.0	8
CTAACGG	75	0.0	46.0	1
CCGTAGG	50	1.6370905E-11	46.0	1
>>END_MODULE