FastQCFastQC Report
Thu 26 May 2016
SRR1544853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544853_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences583256
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT44650.7655300588420866TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT38470.6595731548410989Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT26060.4468020903342615TruSeq Adapter, Index 13 (95% over 22bp)
TGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTTG18730.3211282867214396TruSeq Adapter, Index 13 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT11810.20248398644848917TruSeq Adapter, Index 13 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC10990.18842497976874648No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA10290.17642338870067345No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC9730.16682211584621504TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC9560.16390744372968302No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC7660.13133169654491336No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT7070.12121606978753754Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG700.046.0000044
GCGATAC351.0184158E-746.0000048
ACCACGG351.0184158E-746.0000041
CAAACTA206.3088845E-446.014
GTACCGG253.415072E-546.01
GGAGCGC206.3088845E-446.07
GGTAATC253.415072E-546.07
ATTACGG206.3088845E-446.01
GGCCGAT551.8189894E-1246.07
TAAACGG206.3088845E-446.01
CAACCGG253.415072E-546.01
CGACCAA301.8598148E-646.028
CGATTGA253.415072E-546.09
CGGGCAA1100.046.05
TCCACGA206.3088845E-446.026
CGAATAT405.6024874E-946.014
CTCGCGG600.046.01
GTATCGG206.3088845E-446.01
GCGATAG501.6370905E-1146.08
TCGTAAA206.3088845E-446.033
Produced by FastQC (version 0.11.5)