##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544853_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	583256
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95177760708848	34.0	33.0	34.0	31.0	34.0
2	33.278239743783175	34.0	34.0	34.0	31.0	34.0
3	33.36017632051792	34.0	34.0	34.0	31.0	34.0
4	36.61253891944532	37.0	37.0	37.0	35.0	37.0
5	36.594512529661074	37.0	37.0	37.0	35.0	37.0
6	36.57570432194439	37.0	37.0	37.0	35.0	37.0
7	36.46249159888625	37.0	37.0	37.0	35.0	37.0
8	36.4996828150932	37.0	37.0	37.0	35.0	37.0
9	38.30318933710069	39.0	39.0	39.0	37.0	39.0
10	38.167057004128544	39.0	39.0	39.0	35.0	39.0
11	37.48636619254667	39.0	35.0	39.0	35.0	39.0
12	37.41249811403569	39.0	35.0	39.0	35.0	39.0
13	37.37259796727338	39.0	35.0	39.0	35.0	39.0
14	38.62361981702717	41.0	35.0	41.0	35.0	41.0
15	38.687852332423496	41.0	35.0	41.0	35.0	41.0
16	38.68366034811472	41.0	35.0	41.0	35.0	41.0
17	38.77095134897883	41.0	36.0	41.0	35.0	41.0
18	38.77063587858505	41.0	36.0	41.0	35.0	41.0
19	38.69009491544022	41.0	35.0	41.0	35.0	41.0
20	38.62322205000891	41.0	35.0	41.0	35.0	41.0
21	38.575342559699344	41.0	35.0	41.0	35.0	41.0
22	38.5552896155376	40.0	35.0	41.0	35.0	41.0
23	38.534772038350226	40.0	35.0	41.0	35.0	41.0
24	38.47698266284445	40.0	35.0	41.0	35.0	41.0
25	38.41848347895264	40.0	35.0	41.0	35.0	41.0
26	38.38032356289519	40.0	35.0	41.0	35.0	41.0
27	38.35781200707751	40.0	35.0	41.0	35.0	41.0
28	38.40023420247713	40.0	36.0	41.0	35.0	41.0
29	38.44341592714005	40.0	36.0	41.0	35.0	41.0
30	38.3037019764906	40.0	35.0	41.0	35.0	41.0
31	38.310560028529494	40.0	35.0	41.0	35.0	41.0
32	38.26239935808633	40.0	35.0	41.0	35.0	41.0
33	38.16747705981593	40.0	35.0	41.0	35.0	41.0
34	38.12208704239648	40.0	35.0	41.0	35.0	41.0
35	38.09782496879586	40.0	35.0	41.0	35.0	41.0
36	38.09286488265873	40.0	35.0	41.0	35.0	41.0
37	38.03731637565666	40.0	35.0	41.0	35.0	41.0
38	38.04209643792777	40.0	35.0	41.0	35.0	41.0
39	38.01112033138107	40.0	35.0	41.0	35.0	41.0
40	37.973762807412186	40.0	35.0	41.0	35.0	41.0
41	37.95016939388536	40.0	35.0	41.0	35.0	41.0
42	37.87779979974488	40.0	35.0	41.0	35.0	41.0
43	37.806321752369456	40.0	35.0	41.0	35.0	41.0
44	37.68062566008751	40.0	35.0	41.0	35.0	41.0
45	37.6639983129192	40.0	35.0	41.0	35.0	41.0
46	37.62562408273554	40.0	35.0	41.0	35.0	41.0
47	37.55708128163276	39.0	35.0	41.0	35.0	41.0
48	37.532548314976616	39.0	35.0	41.0	35.0	41.0
49	37.482798290973435	39.0	35.0	41.0	35.0	41.0
50	37.39856769583167	39.0	35.0	41.0	35.0	41.0
51	37.318738255585885	39.0	35.0	41.0	35.0	41.0
52	36.87711913808002	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	8.0
15	7.0
16	15.0
17	18.0
18	32.0
19	72.0
20	114.0
21	179.0
22	325.0
23	552.0
24	870.0
25	1462.0
26	2103.0
27	2453.0
28	2578.0
29	2806.0
30	3265.0
31	4128.0
32	5391.0
33	8055.0
34	25817.0
35	123708.0
36	17316.0
37	26819.0
38	53322.0
39	300011.0
40	1827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.73898939745155	22.32038761710124	25.038233640116864	21.902389345330352
2	24.252986681662943	25.14504780062271	27.53267861796535	23.069286899748995
3	24.609605387685683	25.6172246835009	27.500788675984474	22.272381252828946
4	26.185071392321724	26.214903918690936	24.81071776372639	22.78930692526095
5	24.288648552265215	33.35087851646618	21.28979384695571	21.07067908431289
6	85.91133224518907	2.0723318748542665	8.475352160972198	3.5409837189844597
7	87.66699356714719	1.6896525710837094	5.742589874771969	4.900763986997133
8	77.65560920076263	3.312953488691072	11.893062394557449	7.138374915988862
9	46.71945080719272	17.23737089717037	15.222989562044798	20.82018873359211
10	39.66971621380663	17.40745058773506	27.75916578654999	15.163667411908321
11	37.516973678796276	18.55463124254187	23.86807851097974	20.060316567682115
12	19.4425775302783	34.032397437831754	28.389249317623822	18.13577571426612
13	14.184509032054535	37.02919472752959	30.608686408712472	18.177609831703403
14	13.714389564788018	24.65864045976381	43.59852277559082	18.028447199857354
15	15.627443181038858	19.089216398974035	45.73874936563019	19.54459105435692
16	15.010561400139904	21.833465922339418	28.156589902204175	34.9993827753165
17	18.47987847531787	22.648716858463523	33.54856186648744	25.322842799731166
18	22.611683377453467	27.88483959016281	28.10824063532994	21.39523639705378
19	26.126092144787194	23.335722221460216	25.40016733646975	25.138018297282837
20	19.41291645521006	31.23808413458241	27.4719848574211	21.877014552786427
21	18.94039666972993	24.10588146542856	28.141502187718597	28.812219677122908
22	17.414651542375903	29.100943667960554	24.5490830784424	28.935321711221146
23	17.93757801034194	22.351248851276285	37.70402704815724	22.007146090224534
24	20.03819934985667	21.989143703622425	33.427860150602825	24.544796795918085
25	16.61963185976655	30.82642956094751	29.21735910132086	23.336579477965078
26	17.66822801651419	27.82877501474481	28.553842566557396	25.949154402183606
27	19.052354369264954	25.92377960963968	36.24823405159998	18.775631969495386
28	16.80274184920515	23.102034098234736	34.3939882315827	25.70123582097741
29	22.717640281454457	25.0984130467582	29.139863113281304	23.044083558506042
30	26.38121168063423	22.955443235903275	27.606917031286432	23.05642805217606
31	27.962678480804314	23.44082186895634	26.70645479857901	21.890044851660335
32	27.952219951444988	23.588098536491696	25.203341242953353	23.256340269109963
33	17.758925754728626	21.941308790651103	33.75378907375149	26.54597638086878
34	18.594065041765536	20.888597802680124	32.58500555502215	27.932331600532184
35	26.390984404789663	20.258857174208238	29.574320709945546	23.775837711056553
36	22.575164250346333	23.44133622285926	28.62619501556778	25.35730451122663
37	19.064184509032053	25.682033275268495	29.33308872947728	25.920693486222174
38	18.638642380018382	23.930143881931777	26.631873482655987	30.799340255393858
39	25.601108261209486	19.924012783409	25.966642434882797	28.50823652049872
40	16.528248316348222	18.806150301068485	31.265344891437035	33.40025649114626
41	21.20012481654711	22.988704788291933	25.073038254214275	30.738132140946682
42	21.933250579505398	19.50104242390991	29.659017652625945	28.906689343958742
43	23.234223051284513	23.02728133101074	29.227646179379207	24.51084943832554
44	19.19620201078086	27.06907430013579	27.15359979151522	26.581123897568137
45	20.752636921008957	24.014669373311204	28.755983650403937	26.4767100552759
46	23.049912902739106	19.52367399563828	28.70471971141317	28.721693390209445
47	20.218223215877764	17.439169078414967	35.079450532870645	27.263157172836628
48	20.282860356344383	16.657008243378552	30.951417559356443	32.10871384092063
49	20.235025443373065	21.570973980550566	31.837820785384118	26.356179790692252
50	18.959599215438846	23.47545503175278	30.1999465071941	27.364999245614275
51	19.3765687794039	18.476963803201336	30.683439175936467	31.463028241458296
52	21.3652324193836	17.711776646961198	35.16689069636661	25.7561002372886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	108.5
2	154.0
3	788.0
4	1422.0
5	1013.0
6	604.0
7	632.0
8	660.0
9	698.5
10	737.0
11	764.5
12	792.0
13	744.0
14	694.0
15	692.0
16	689.5
17	687.0
18	630.5
19	574.0
20	597.0
21	620.0
22	997.5
23	1375.0
24	1555.5
25	1736.0
26	1950.0
27	2164.0
28	2367.0
29	2570.0
30	3430.0
31	4290.0
32	4882.5
33	5475.0
34	7017.5
35	8560.0
36	9252.5
37	9945.0
38	11148.5
39	14065.5
40	15779.0
41	21232.5
42	26686.0
43	35641.0
44	44596.0
45	48153.0
46	51710.0
47	53922.5
48	56135.0
49	64814.5
50	73494.0
51	74601.5
52	75709.0
53	65563.5
54	55418.0
55	46374.5
56	37331.0
57	31202.0
58	25073.0
59	21655.5
60	18238.0
61	16989.5
62	15741.0
63	14035.5
64	10274.5
65	8219.0
66	6308.0
67	4397.0
68	3732.0
69	3067.0
70	2394.5
71	1722.0
72	1179.5
73	637.0
74	514.5
75	392.0
76	317.5
77	243.0
78	180.5
79	118.0
80	69.5
81	21.0
82	15.5
83	10.0
84	13.0
85	16.0
86	10.5
87	5.0
88	2.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	583256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.47950035384446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4478989247159	19.08393563075485
2	12.543852315841256	6.893975883034649
3	4.631306045146685	3.8179792831911095
4	2.2781867694951305	2.5041373655386083
5	1.216095415026188	1.6708847193760379
6	0.7899661900737383	1.302472571979387
7	0.564816277626595	1.086460837062799
8	0.44935382840790694	0.9878414949389155
9	0.38284114369219696	0.946825500920035
>10	7.282141243758488	47.68591252087065
>50	0.30718463917096095	5.655829788703079
>100	0.09697274759979418	4.640641178383555
>500	0.005005045037408732	0.9551884706071587
>1k	0.004379414407732641	2.7679147546391745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	4465	0.7655300588420866	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT	3847	0.6595731548410989	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	2606	0.4468020903342615	TruSeq Adapter, Index 13 (95% over 22bp)
TGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTTG	1873	0.3211282867214396	TruSeq Adapter, Index 13 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	1181	0.20248398644848917	TruSeq Adapter, Index 13 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1099	0.18842497976874648	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1029	0.17642338870067345	No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	973	0.16682211584621504	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	956	0.16390744372968302	No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	766	0.13133169654491336	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	707	0.12121606978753754	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6732892589188967	0.0
2	0.0	0.0	0.0	2.248412360952995	0.0
3	0.0	0.0	0.0	3.4736033577022782	0.0
4	0.0	0.0	0.0	4.170381444854404	0.0
5	0.0	0.0	0.0	7.787832444072586	0.0
6	0.0	0.0	0.0	8.360994143223559	0.0
7	0.0	0.0	0.0	10.622951156953379	0.0
8	0.0	0.0	0.0	13.132826751889393	0.0
9	0.0	0.0	0.0	15.170868366549165	0.0
10	0.0	0.0	0.0	16.558252294018406	0.0
11	0.0	0.0	0.0	17.787901024592976	0.0
12	0.0	0.0	0.0	18.89376191586542	0.0
13	0.0	0.0	0.0	19.30884551551977	0.0
14	0.0	0.0	0.0	19.640775234202476	0.0
15	0.0	0.0	0.0	20.777325908348992	0.0
16	0.0	0.0	0.0	22.01314688575857	0.0
17	0.0	0.0	0.0	23.63078991043384	0.0
18	0.0	0.0	0.0	24.34797070240169	0.0
19	0.0	0.0	0.0	25.126702511418657	0.0
20	0.0	0.0	0.0	26.16586884661281	0.0
21	0.0	0.0	0.0	26.992607019902067	0.0
22	0.0	0.0	0.0	27.958563649580974	0.0
23	0.0	0.0	0.0	28.652255613315592	0.0
24	0.0	0.0	0.0	29.21101540318488	0.0
25	0.0	0.0	0.0	29.776633245093063	0.0
26	0.0	0.0	0.0	30.263554939854885	0.0
27	0.0	0.0	0.0	30.75853484576241	0.0
28	0.0	0.0	0.0	31.267059404446762	0.0
29	0.0	0.0	0.0	31.96075136818138	0.0
30	0.0	0.0	0.0	32.591006350556185	0.0
31	0.0	0.0	0.0	33.10073106834734	0.0
32	0.0	0.0	0.0	33.609769980934615	0.0
33	0.0	0.0	0.0	34.12686710466759	0.0
34	0.0	0.0	0.0	34.840104516713076	0.0
35	0.0	0.0	0.0	35.347086013688674	0.0
36	0.0	0.0	0.0	35.82903562072229	0.0
37	0.0	0.0	0.0	36.3521335399893	0.0
38	0.0	0.0	0.0	36.81985268904221	0.0
39	0.0	0.0	0.0	37.40604468706709	0.0
40	0.0	0.0	0.0	37.97869203231514	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	70	0.0	46.000004	4
GCGATAC	35	1.0184158E-7	46.000004	8
ACCACGG	35	1.0184158E-7	46.000004	1
CAAACTA	20	6.3088845E-4	46.0	14
GTACCGG	25	3.415072E-5	46.0	1
GGAGCGC	20	6.3088845E-4	46.0	7
GGTAATC	25	3.415072E-5	46.0	7
ATTACGG	20	6.3088845E-4	46.0	1
GGCCGAT	55	1.8189894E-12	46.0	7
TAAACGG	20	6.3088845E-4	46.0	1
CAACCGG	25	3.415072E-5	46.0	1
CGACCAA	30	1.8598148E-6	46.0	28
CGATTGA	25	3.415072E-5	46.0	9
CGGGCAA	110	0.0	46.0	5
TCCACGA	20	6.3088845E-4	46.0	26
CGAATAT	40	5.6024874E-9	46.0	14
CTCGCGG	60	0.0	46.0	1
GTATCGG	20	6.3088845E-4	46.0	1
GCGATAG	50	1.6370905E-11	46.0	8
TCGTAAA	20	6.3088845E-4	46.0	33
>>END_MODULE