FastQCFastQC Report
Thu 26 May 2016
SRR1544852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544852_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1451807
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT40020.27565647499977614TruSeq Adapter, Index 14 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT32400.22317015966998366Illumina PCR Primer Index 3 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31090.21414692173270963No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27800.19148550737115883No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT25030.17240583631295345TruSeq Adapter, Index 14 (96% over 26bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA19050.13121578832448114No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAAC206.312005E-446.042
ATGTACG206.312005E-446.06
ACAATCG206.312005E-446.01
ACGCTAA206.312005E-446.010
CGCAAAT206.312005E-446.044
TGTACGA206.312005E-446.038
GATTACG206.312005E-446.010
GTACGAC206.312005E-446.044
TAGTAGG3950.044.2531661
CGGTCTA1000.043.730
ACGTAGG1900.043.578951
ACGGGTA850.043.2941174
CATGCGG4150.042.6746981
ACTACGG650.042.461541
CTAGCGG4250.042.2117651
ATTGCGG3900.041.8717961
ATCAACG9300.041.79569614
CGTTTTT17500.041.5314251
CGCAAGC9250.041.52432319
ATAGCGG2450.041.306121
Produced by FastQC (version 0.11.5)